Restriction map for pF1KB0977
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HindIII     HpaII       KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tsp45I      Tsp509I     
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI           22  GTAC                 GT^AC
AluI          413  AGCT                 AG^CT
Asp700I       359  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          337  CCCGAG               C^[CT]CG[GA]G
AvrII          31  CCTAGG               C^CTAGG
BbvI          414  GCTGC                GCAGC(8/12)
BcgI           75  CGATGATATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI           31  CCTAGG               C^CTAGG
BpmI          310  CTCCAG               CTGGAG(16/14)
BsaI           27  GAGACC               GGTCTC(1/5)
BsmBI           8  GAGACG               CGTCTC(1/5)
BsoBI         337  CCCGAG               C^[CT]CG[GA]G
BsrI          519  ACTGG                ACTGG(1/-1)
BstF5I        286  CATCC                GGATG(2/0)
CfoI          417  GCGC                 GCG^C
DraIII        496  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          267  CCGTGG               C^C[GA][CT]GG
EcoT14I        31  CCTAGG               C^C[AT][AT]GG
Fnu4HI        414  GCTGC                GC^[ACGT]GC
FokI          286  CATCC                GGATG(9/13)
HhaI          417  GCGC                 GCG^C
HinP1I        417  GCGC                 G^CGC
HincII        451  GTTAAC               GT[CT]^[GA]AC
HindII        451  GTTAAC               GT[CT]^[GA]AC
HpaI          451  GTTAAC               GTT^AAC
HphI          522  GGTGA                GGTGA(8/7)
ItaI          414  GCTGC                GC^[ACGT]GC
MseI          452  TTAA                 T^TAA
MslI          138  CATCTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        412  CAGCTG               C[AC]G^C[GT]G
MwoI          336  GCCCGAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          483  ACATGC               [GA]CATG^[CT]
PvuII         412  CAGCTG               CAG^CTG
RsaI           22  GTAC                 GT^AC
StuI           68  AGGCCT               AGG^CCT
StyI           31  CCTAGG               C^C[AT][AT]GG
TaqI           74  TCGA                 T^CGA
Tru9I         452  TTAA                 T^TAA
TseI          414  GCTGC                G^C[AT]GC
TthHB8I        74  TCGA                 T^CGA
XmnI          359  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AocI          422  CCTCAGG              CC^T[ACGT]AGG
              547  CCTCAGG             
AvaII         466  GGACC                G^G[AT]CC
              491  GGACC               
BfaI           32  CTAG                 C^TAG
              181  CTAG                
BsmAI           8  GAGAC                GTCTC(1/5)
               27  GAGAC               
Bsu36I        422  CCTCAGG              CC^T[ACGT]AGG
              547  CCTCAGG             
Cac8I         305  GCAGGC               GC[ACGT]^[ACGT]GC
              345  GCCAGC              
DpnII         330  GATC                 ^GATC
              537  GATC                
EarI          153  GAAGAG               CTCTTC(1/4)
              532  GAAGAG              
Eco81I        422  CCTCAGG              CC^T[ACGT]AGG
              547  CCTCAGG             
Ksp632I       153  GAAGAG               CTCTTC(1/4)
              532  GAAGAG              
MaeI           32  CTAG                 C^TAG
              181  CTAG                
MboI          330  GATC                 ^GATC
              537  GATC                
NdeII         330  GATC                 ^GATC
              537  GATC                
Sau3AI        330  GATC                 ^GATC
              537  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BssKI       BstNI       
Cfr13I      DdeI        EcoRII      HaeIII      HinfI       
MboII       MnlI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       TfiI        
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