Restriction map for pF1KB0977
 (Linear) (Six-base) MAPSORT of: KIBB0977.seq  Check: 5267  from: 1  to: 564



KIBB0977.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AvaI C'yCGr_G

Cuts at:      0     337     564 
   Size:        337     227
 
AvrII C'CTAG_G

Cuts at:      0      31     564 
   Size:         31     533
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      64      98     564 
   Size:         64      34     466

  Fragments arranged by size: 

                 466      64      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     564 
   Size:         21     543
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     564 
   Size:          2     562
 
Bsu36I CC'TnA_GG

Cuts at:      0     423     548     564 
   Size:        423     125      16

  Fragments arranged by size: 

                 423     125      16
 
BtgI C'CryG_G

Cuts at:      0     267     564 
   Size:        267     297
 
DraIII CAC_nnn'GTG

Cuts at:      0     501     564 
   Size:        501      63
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     527     564 
   Size:        148     379      37

  Fragments arranged by size: 

                 379     148      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
HincII GTy'rAC

Cuts at:      0     453     564 
   Size:        453     111
 
HpaI GTT'AAC

Cuts at:      0     453     564 
   Size:        453     111
 
MslI CAynn'nnrTG

Cuts at:      0     142     564 
   Size:        142     422
 
MspA1I CmG'CkG

Cuts at:      0     414     564 
   Size:        414     150
 
NspI r_CATG'y

Cuts at:      0     487     564 
   Size:        487      77
 
PvuII CAG'CTG

Cuts at:      0     414     564 
   Size:        414     150
 
StuI AGG'CCT

Cuts at:      0      70     564 
   Size:         70     494
 
StyI C'CwwG_G

Cuts at:      0      31     564 
   Size:         31     533
 
XmnI GAAnn'nnTTC

Cuts at:      0     363     564 
   Size:        363     201
 

 Enzymes that do cut:

     AvaI    AvrII     BcgI     BpmI     BsaI    BsmBI   Bsu36I     BtgI
   DraIII     EarI  Eco57MI   HincII     HpaI     MslI   MspA1I     NspI
    PvuII     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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