Restriction map for
pF1KB0975
(Linear) (Six-base) MAPSORT of: KIBB0975.seq Check: 4217 from: 1 to: 633
KIBB0975.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 64 504 633
Size: 64 440 129
Fragments arranged by size:
440 129 64
AatII G_ACGT'C
Cuts at: 0 205 633
Size: 205 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 633
Size: 559 74
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 633
Size: 400 32 201
Fragments arranged by size:
400 201 32
ApaI G_GGCC'C
Cuts at: 0 532 633
Size: 532 101
ApaLI G'TGCA_C
Cuts at: 0 212 292 633
Size: 212 80 341
Fragments arranged by size:
341 212 80
AvaI C'yCGr_G
Cuts at: 0 229 508 633
Size: 229 279 125
Fragments arranged by size:
279 229 125
AvrII C'CTAG_G
Cuts at: 0 196 633
Size: 196 437
BanI G'GyrC_C
Cuts at: 0 84 633
Size: 84 549
BanII G_rGCy'C
Cuts at: 0 532 633
Size: 532 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 565 633
Size: 565 68
BclI T'GATC_A
Cuts at: 0 260 633
Size: 260 373
Bme1580I G_kGCm'C
Cuts at: 0 216 296 532 633
Size: 216 80 236 101
Fragments arranged by size:
236 216 101 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 633
Size: 433 200
BsaHI Gr'CG_yC
Cuts at: 0 202 633
Size: 202 431
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 633
Size: 400 30 203
Fragments arranged by size:
400 203 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 534 633
Size: 138 396 99
Fragments arranged by size:
396 138 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 400 633
Size: 98 302 233
Fragments arranged by size:
302 233 98
BsiHKAI G_wGCw'C
Cuts at: 0 175 216 296 526 633
Size: 175 41 80 230 107
Fragments arranged by size:
230 175 107 80 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 209 436 633
Size: 209 227 197
Fragments arranged by size:
227 209 197
Bsp1286I G_dGCh'C
Cuts at: 0 175 216 296 526 532 633
Size: 175 41 80 230 6 101
Fragments arranged by size:
230 175 101 80 41 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 504 633
Size: 64 440 129
Fragments arranged by size:
440 129 64
BsrFI r'CCGG_y
Cuts at: 0 90 633
Size: 90 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 314 340 633
Size: 314 26 293
Fragments arranged by size:
314 293 26
DraIII CAC_nnn'GTG
Cuts at: 0 436 633
Size: 436 197
EarI CTCTTCn'nnn_
Cuts at: 0 620 633
Size: 620 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 633
Size: 65 568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 559 633
Size: 433 126 74
Fragments arranged by size:
433 126 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 195 341 633
Size: 195 146 292
Fragments arranged by size:
292 195 146
EcoO109I rG'GnC_Cy
Cuts at: 0 117 633
Size: 117 516
MslI CAynn'nnrTG
Cuts at: 0 598 633
Size: 598 35
MspA1I CmG'CkG
Cuts at: 0 289 633
Size: 289 344
NaeI GCC'GGC
Cuts at: 0 92 633
Size: 92 541
NgoMIV G'CCGG_C
Cuts at: 0 90 633
Size: 90 543
PflMI CCAn_nnn'nTGG
Cuts at: 0 46 633
Size: 46 587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 633
Size: 400 32 201
Fragments arranged by size:
400 201 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 633
Size: 117 516
PspOMI G'GGCC_C
Cuts at: 0 528 633
Size: 528 105
SanDI GG'GwC_CC
Cuts at: 0 117 633
Size: 117 516
SapI GCTCTTCn'nnn_
Cuts at: 0 620 633
Size: 620 13
SfcI C'TryA_G
Cuts at: 0 312 633
Size: 312 321
SgrAI Cr'CCGG_yG
Cuts at: 0 90 633
Size: 90 543
SmaI CCC'GGG
Cuts at: 0 510 633
Size: 510 123
StyI C'CwwG_G
Cuts at: 0 45 196 633
Size: 45 151 437
Fragments arranged by size:
437 151 45
XbaI T'CTAG_A
Cuts at: 0 339 633
Size: 339 294
XmaI C'CCGG_G
Cuts at: 0 508 633
Size: 508 125
ZraI GAC'GTC
Cuts at: 0 203 633
Size: 203 430
Enzymes that do cut:
AarI AatII AcuI AloI ApaI ApaLI AvaI AvrII
BanI BanII BbsI BclI Bme1580I BpmI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BtgZI
DraIII EarI EciI Eco57MI EcoNI EcoO109I MslI MspA1I
NaeI NgoMIV PflMI PpiI PpuMI PspOMI SanDI SapI
SfcI SgrAI SmaI StyI XbaI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraI DrdI EaeI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XcmI XhoI XmnI