Restriction map for pF1KB0974
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# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BbrPI       BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HindIII     
HphI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcsI          813  AAATTC               [GA]^AATT[CT]
AcyI         1039  GGCGCC               G[GA]^CG[CT]C
AfaI         1062  GTAC                 GT^AC
AflIII        170  ACACGT               A^C[GA][CT]GT
AlwNI          58  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          486  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1278  AGCGCT               AGC^GCT
ApoI          813  AAATTC               [GA]^AATT[CT]
Asp700I       998  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         385  GGATCC               G^GATCC
BbeI         1039  GGCGCC               GGCGC^C
BbsI          822  GAAGAC               GAAGAC(2/6)
BbvCI        1228  GCTGAGG              CCTCAGC(-5/-2)
BglI         1031  GCCCCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         446  AGATCT               A^GATCT
BpuAI         822  GAAGAC               GAAGAC(2/6)
BsaHI        1039  GGCGCC               G[GA]^CG[CT]C
BsaI          962  GAGACC               GGTCTC(1/5)
BsaWI         266  ACCGGA               [AT]^CCGG[AT]
BsmBI         736  GAGACG               CGTCTC(1/5)
BsmI         1103  GCATTC               GAATGC(1/-1)
BsrBI         189  GAGCGG               CCGCTC(-3/-3)
BsrDI         120  GCAATG               GCAATG(2/0)
BsrFI         965  ACCGGC               [GA]^CCGG[CT]
BstAPI         57  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1276  CCAGCGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        486  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        965  ACCGGC               [GA]^CCGG[CT]
DsaI          587  CCGTGG               C^C[GA][CT]GG
EarI          442  GAAGAG               CTCTTC(1/4)
Eco47III     1278  AGCGCT               AGC^GCT
Eco81I        486  CCTCAGG              CC^T[ACGT]AGG
EcoT22I       534  ATGCAT               ATGCA^T
Hin1I        1039  GGCGCC               G[GA]^CG[CT]C
HincII        340  GTTAAC               GT[CT]^[GA]AC
HindII        340  GTTAAC               GT[CT]^[GA]AC
HpaI          340  GTTAAC               GTT^AAC
KasI         1039  GGCGCC               G^GCGCC
Ksp632I       442  GAAGAG               CTCTTC(1/4)
MaeII         172  ACGT                 A^CGT
MaeIII        256  GTCAC                ^GT[ACGT]AC
MseI          341  TTAA                 T^TAA
NarI         1039  GGCGCC               GG^CGCC
NsiI          534  ATGCAT               ATGCA^T
PflMI         680  CCACTGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          792  CTGCAG               CTGCA^G
PvuII         141  CAGCTG               CAG^CTG
RsaI         1062  GTAC                 GT^AC
SfcI          792  CTGCAG               C^T[GA][CT]AG
SfoI         1039  GGCGCC               GGC^GCC
SphI          536  GCATGC               GCATG^C
TaqI          697  TCGA                 T^CGA
Tru9I         341  TTAA                 T^TAA
Tsp45I        256  GTCAC                ^GT[GC]AC
TthHB8I       697  TCGA                 T^CGA
Van91I        680  CCACTGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1276  CCAGCGCTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          998  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AspHI         211  GTGCTC               G[AT]GC[AT]^C
              262  GAGCAC              
BcgI         1021  CGAATGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              329  GCAACAACCTCG        
BcnI          116  CCCGG                CC^[GC]GG
              277  CCCGG               
BpmI          427  CTGGAG               CTGGAG(16/14)
             1237  CTGGAG              
BsiHKAI       211  GTGCTC               G[AT]GC[AT]^C
              262  GAGCAC              
EcoNI         952  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1028  CCTGCCCCAGG         
FauI          251  CCCGC                CCCGC(4/6)
              351  CCCGC               
HgaI          738  GACGC                GACGC(5/10)
              254  GCGTC               
MspA1I        141  CAGCTG               C[AC]G^C[GT]G
              150  CAGCGG              
NciI          116  CCCGG                CC^[GC]GG
              277  CCCGG               
NspI           90  GCATGT               [GA]CATG^[CT]
              536  GCATGC              
Tsp509I       579  AATT                 ^AATT
              814  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        ApaI        
AvaII       BanI        BanII       BbvI        BmyI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TseI        TspRI       
XhoII       
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