Restriction map for pF1KB0974
 (Linear) (Six-base) MAPSORT of: KIBB0974.seq  Check: 729  from: 1  to: 1311



KIBB0974.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     714    1311 
   Size:        714     597
 
AfeI AGC'GCT

Cuts at:      0    1280    1311 
   Size:       1280      31
 
AflIII A'CryG_T

Cuts at:      0     170    1311 
   Size:        170    1141
 
AleI CACnn'nnGTG

Cuts at:      0     304    1311 
   Size:        304    1007
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     937    1217    1311 
   Size:        937     280      94

  Fragments arranged by size: 

                 937     280      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0      63    1311 
   Size:         63    1248
 
ApaI G_GGCC'C

Cuts at:      0     277     363     587    1311 
   Size:        277      86     224     724

  Fragments arranged by size: 

                 724     277     224      86
 
ApoI r'AATT_y

Cuts at:      0     813    1311 
   Size:        813     498
 
BamHI G'GATC_C

Cuts at:      0     385    1311 
   Size:        385     926
 
BanI G'GyrC_C

Cuts at:      0     161     395    1039    1311 
   Size:        161     234     644     272

  Fragments arranged by size: 

                 644     272     234     161
 
BanII G_rGCy'C

Cuts at:      0     251     277     363     587     810    1311 
   Size:        251      26      86     224     223     501

  Fragments arranged by size: 

                 501     251     224     223      86      26
 
BbeI G_GCGC'C

Cuts at:      0    1043    1311 
   Size:       1043     268
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     829    1311 
   Size:        829     482
 
BbvCI CC'TCA_GC

Cuts at:      0    1229    1311 
   Size:       1229      82
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     318     352    1010    1044    1311 
   Size:        318      34     658      34     267

  Fragments arranged by size: 

                 658     318     267      34      34
 
BfrBI ATG'CAT

Cuts at:      0     536    1311 
   Size:        536     775
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1037    1311 
   Size:       1037     274
 
BglII A'GATC_T

Cuts at:      0     446    1311 
   Size:        446     865
 
Bme1580I G_kGCm'C

Cuts at:      0     277     363     400     587    1311 
   Size:        277      86      37     187     724

  Fragments arranged by size: 

                 724     277     187      86      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394    1311 
   Size:        394     917
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1258    1311 
   Size:        448     810      53

  Fragments arranged by size: 

                 810     448      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     493    1229    1311 
   Size:        493     736      82

  Fragments arranged by size: 

                 736     493      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     656     926    1311 
   Size:        411     245     270     385

  Fragments arranged by size: 

                 411     385     270     245
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     956    1311 
   Size:        956     355
 
BsaHI Gr'CG_yC

Cuts at:      0    1040    1311 
   Size:       1040     271
 
BsaWI w'CCGG_w

Cuts at:      0     266    1311 
   Size:        266    1045
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     865     895    1311 
   Size:        865      30     416

  Fragments arranged by size: 

                 865     416      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475     515     873     929    1311 
   Size:        475      40     358      56     382

  Fragments arranged by size: 

                 475     382     358      56      40
 
BseYI C'CCAG_C

Cuts at:      0     372     542    1275    1311 
   Size:        372     170     733      36

  Fragments arranged by size: 

                 733     372     170      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     210    1006    1213    1311 
   Size:         76     134     796     207      98

  Fragments arranged by size: 

                 796     207     134      98      76
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     266    1311 
   Size:        215      51    1045

  Fragments arranged by size: 

                1045     215      51
 
BsmI GAATG_Cn'

Cuts at:      0    1103    1311 
   Size:       1103     208
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     730    1311 
   Size:        730     581
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     251     266     277     363     400     587     810 
   Size:        215      36      15      11      86      37     187     223

Cuts at:    810    1311 
   Size:        501

  Fragments arranged by size: 

                 501     223     215     187      86      37      36      15
                  11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     119     566     714    1036    1311 
   Size:        119     447     148     322     275

  Fragments arranged by size: 

                 447     322     275     148     119
 
BsrBI CCG'CTC

Cuts at:      0     191    1311 
   Size:        191    1120
 
BsrDI GCAATG_nn'

Cuts at:      0     127    1311 
   Size:        127    1184
 
BsrFI r'CCGG_y

Cuts at:      0     965    1311 
   Size:        965     346
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      63    1311 
   Size:         63    1248
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1283    1311 
   Size:       1283      28
 
BstYI r'GATC_y

Cuts at:      0     385     446    1163    1311 
   Size:        385      61     717     148

  Fragments arranged by size: 

                 717     385     148      61
 
Bsu36I CC'TnA_GG

Cuts at:      0     487    1311 
   Size:        487     824
 
BtgI C'CryG_G

Cuts at:      0     587    1311 
   Size:        587     724
 
BtsI GCAGTG_nn'

Cuts at:      0      43     976    1311 
   Size:         43     933     335

  Fragments arranged by size: 

                 933     335      43
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     758     793    1311 
   Size:        758      35     518

  Fragments arranged by size: 

                 758     518      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     437    1311 
   Size:        437     874
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1258    1311 
   Size:        448     810      53

  Fragments arranged by size: 

                 810     448      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     956    1032    1311 
   Size:        956      76     279

  Fragments arranged by size: 

                 956     279      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0     274     360    1233    1311 
   Size:        274      86     873      78

  Fragments arranged by size: 

                 873     274      86      78
 
HaeII r_GCGC'y

Cuts at:      0      64    1043    1282    1311 
   Size:         64     979     239      29

  Fragments arranged by size: 

                 979     239      64      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     491     523     618     650    1311 
   Size:        370      32      89      32      95      32     661

  Fragments arranged by size: 

                 661     370      95      89      32      32      32
 
HincII GTy'rAC

Cuts at:      0     342    1311 
   Size:        342     969
 
HpaI GTT'AAC

Cuts at:      0     342    1311 
   Size:        342     969
 
KasI G'GCGC_C

Cuts at:      0    1039    1311 
   Size:       1039     272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     131    1311 
   Size:        131    1180
 
MslI CAynn'nnrTG

Cuts at:      0     175     304     473     649    1052    1096    1311 
   Size:        175     129     169     176     403      44     215

  Fragments arranged by size: 

                 403     215     176     175     169     129      44
 
MspA1I CmG'CkG

Cuts at:      0     143     152    1311 
   Size:        143       9    1159

  Fragments arranged by size: 

                1159     143       9
 
NarI GG'CG_CC

Cuts at:      0    1040    1311 
   Size:       1040     271
 
NsiI A_TGCA'T

Cuts at:      0     538    1311 
   Size:        538     773
 
NspI r_CATG'y

Cuts at:      0      94     540    1311 
   Size:         94     446     771

  Fragments arranged by size: 

                 771     446      94
 
PasI CC'CwG_GG

Cuts at:      0    1034    1311 
   Size:       1034     277
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     686    1311 
   Size:        686     625
 
PspOMI G'GGCC_C

Cuts at:      0     273     359     583    1311 
   Size:        273      86     224     728

  Fragments arranged by size: 

                 728     273     224      86
 
PstI C_TGCA'G

Cuts at:      0     796    1311 
   Size:        796     515
 
PvuII CAG'CTG

Cuts at:      0     143    1311 
   Size:        143    1168
 
SfcI C'TryA_G

Cuts at:      0     792    1311 
   Size:        792     519
 
SfoI GGC'GCC

Cuts at:      0    1041    1311 
   Size:       1041     270
 
SmlI C'TyrA_G

Cuts at:      0     390     671     941    1311 
   Size:        390     281     270     370

  Fragments arranged by size: 

                 390     370     281     270
 
SphI G_CATG'C

Cuts at:      0     540    1311 
   Size:        540     771
 
StyI C'CwwG_G

Cuts at:      0     218     236     455     993    1311 
   Size:        218      18     219     538     318

  Fragments arranged by size: 

                 538     318     219     218      18
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     185     217     865     897    1311 
   Size:        185      32     648      32     414

  Fragments arranged by size: 

                 648     414     185      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1283    1311 
   Size:       1283      28
 
XmnI GAAnn'nnTTC

Cuts at:      0    1002    1311 
   Size:       1002     309
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI     ApoI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     EarI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     HpaI     KasI     MmeI     MslI
   MspA1I     NarI     NsiI     NspI     PasI    PflMI   PspOMI     PstI
    PvuII     SfcI     SfoI     SmlI     SphI     StyI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BciVI     BclI     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsiEI    BsiWI    BspEI    BspHI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com