Restriction map for pF1KB0961
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp45I      Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI          385  GCGG                 CCGC(-3/-1)
AcsI          322  AAATTT               [GA]^AATT[CT]
AfaI          137  GTAC                 GT^AC
Aor51HI       350  AGCGCT               AGC^GCT
ApoI          322  AAATTT               [GA]^AATT[CT]
Asp700I        61  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         567  GGTCC                G^G[AT]CC
BalI          288  TGGCCA               TGG^CCA
BanI          232  GGCACC               G^G[CT][GA]CC
BbsI           42  GAAGAC               GAAGAC(2/6)
BbvCI         237  CCTCAGC              CCTCAGC(-5/-2)
BpuAI          42  GAAGAC               GAAGAC(2/6)
BsrI            8  ACTGG                ACTGG(1/-1)
BssSI         274  CACGAG               CACGAG(-5/-1)
BstXI         330  CCAGAAGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         559  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EarI           61  GAAGAG               CTCTTC(1/4)
Eco47III      350  AGCGCT               AGC^GCT
EcoO109I      559  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       314  ATGCAT               ATGCA^T
HaeII         350  AGCGCT               [GA]GCGC^[CT]
HincII        462  GTCAAC               GT[CT]^[GA]AC
HindII        462  GTCAAC               GT[CT]^[GA]AC
Ksp632I        61  GAAGAG               CTCTTC(1/4)
MluNI         288  TGGCCA               TGG^CCA
MscI          288  TGGCCA               TGG^CCA
NsiI          314  ATGCAT               ATGCA^T
RsaI          137  GTAC                 GT^AC
SmlI          457  CTTGAG               C^T[CT][GA]AG
TaqI          432  TCGA                 T^CGA
TfiI          126  GAATC                G^A[AT]TC
Tsp509I       323  AATT                 ^AATT
TthHB8I       432  TCGA                 T^CGA
XcmI          330  CCAGAAGATTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           61  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI           85  AGCT                 AG^CT
              555  AGCT                
ApaI          199  GGGCCC               GGGCC^C
              526  GGGCCC              
BanII         199  GGGCCC               G[GA]GC[CT]^C
              526  GGGCCC              
BmyI          199  GGGCCC               G[AGT]GC[ACT]^C
              526  GGGCCC              
BseRI          14  GAGGAG               GAGGAG(10/8)
              160  GAGGAG              
BsiYI           4  CCGAACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              105  CCTGTGCATGG         
BslI            4  CCGAACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              105  CCTGTGCATGG         
Bsp1286I      199  GGGCCC               G[AGT]GC[ACT]^C
              526  GGGCCC              
Cac8I         229  GCAGGC               GC[ACGT]^[ACGT]GC
              424  GCCTGC              
CfoI          227  GCGC                 GCG^C
              351  GCGC                
DdeI          238  CTCAG                C^T[ACGT]AG
              575  CTGAG               
EaeI          146  TGGCCG               [CT]^GGCC[GA]
              288  TGGCCA              
EcoT14I       530  CCAAGG               C^C[AT][AT]GG
              563  CCTTGG              
HhaI          227  GCGC                 GCG^C
              351  GCGC                
HinP1I        227  GCGC                 G^CGC
              351  GCGC                
HphI          157  GGTGA                GGTGA(8/7)
              393  GGTGA               
NlaIII        111  CATG                 CATG^
              154  CATG                
PleI          256  GAGTC                GAGTC(4/5)
              460  GAGTC               
SfaNI         362  GATGC                GCATC(5/9)
              316  GCATC               
StuI          422  AGGCCT               AGG^CCT
              453  AGGCCT              
StyI          530  CCAAGG               C^C[AT][AT]GG
              563  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

BbvI        BsaJI       BssKI       BstAPI      BstF5I      
BstNI       Cfr13I      EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MboII       MnlI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com