################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tru9I TseI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 472 GTCTAC GT^[AC][GT]AC AfaI 376 GTAC GT^AC AluI 436 AGCT AG^CT AlwI 5 GATCC GGATC(4/5) AvaII 284 GGACC G^G[AT]CC BalI 29 TGGCCA TGG^CCA BfaI 274 CTAG C^TAG BpmI 357 CTCCAG CTGGAG(16/14) BsaI 389 GGTCTC GGTCTC(1/5) BsmAI 390 GTCTC GTCTC(1/5) BsmI 163 GCATTC GAATGC(1/-1) BstYI 4 AGATCC [GA]^GATC[CT] Cac8I 106 GCCAGC GC[ACGT]^[ACGT]GC EaeI 29 TGGCCA [CT]^GGCC[GA] EarI 429 GAAGAG CTCTTC(1/4) EcoNI 262 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 217 GATATC GAT^ATC EcoT14I 385 CCAAGG C^C[AT][AT]GG HphI 307 GGTGA GGTGA(8/7) Ksp632I 429 GAAGAG CTCTTC(1/4) MaeI 274 CTAG C^TAG MaeII 443 ACGT A^CGT MflI 4 AGATCC [GA]^GATC[CT] MluNI 29 TGGCCA TGG^CCA MscI 29 TGGCCA TGG^CCA MwoI 480 GCCTGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 476 ACATGC [GA]CATG^[CT] PstI 487 CTGCAG CTGCA^G RsaI 376 GTAC GT^AC SfaNI 91 GATGC GCATC(5/9) SfcI 487 CTGCAG C^T[GA][CT]AG SspI 124 AATATT AAT^ATT StyI 385 CCAAGG C^C[AT][AT]GG XhoII 4 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BsaJI 262 CCTGGG C^C[ACGT][ACGT]GG 385 CCAAGG BssKI 262 CCTGG ^CC[ACGT]GG 481 CCTGG BstNI 262 CCTGG CC^[AT]GG 481 CCTGG Cfr13I 284 GGACC G^G[ACGT]CC 319 GGGCC DpnII 5 GATC ^GATC 297 GATC EcoRII 262 CCTGG ^CC[AT]GG 481 CCTGG HaeIII 30 GGCC GG^CC 320 GGCC HinfI 250 GAGTC G^A[ACGT]TC 355 GACTC MboI 5 GATC ^GATC 297 GATC MvaI 262 CCTGG CC^[AT]GG 481 CCTGG NdeII 5 GATC ^GATC 297 GATC NlaIII 137 CATG CATG^ 477 CATG PleI 355 GACTC GAGTC(4/5) 250 GAGTC Sau3AI 5 GATC ^GATC 297 GATC Sau96I 284 GGACC G^G[ACGT]CC 319 GGGCC ScrFI 262 CCTGG CC^[ACGT]GG 481 CCTGG TaqI 246 TCGA T^CGA 299 TCGA TspRI 156 CAGTG CA[GC]TG[ACGT][ACGT]^ 490 CAGTG TthHB8I 246 TCGA T^CGA 299 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### BsiYI BslI BsrI BstF5I DdeI FokI MboII MnlI