Restriction map for pF1KB0475
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AseI        
AsnI        Asp700I     AspEI       AspHI       AvaI        
AviII       AvrII       BamHI       BanII       BbsI        
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsiEI       BsiHKAI     BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRV       
EcoT22I     FbaI        FspI        HindIII     HpaI        
KspI        MaeI        MamI        MluI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          361  GTCTAC               GT^[AC][GT]AC
AccIII        593  TCCGGA               T^CCGGA
AflIII       1181  ACGTGT               A^C[GA][CT]GT
AocI          739  CCTGAGG              CC^T[ACGT]AGG
AscI         1040  GGCGCGCC             GG^CGCGCC
AspI          493  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1180  CACGTG               CAC^GTG
BbvCI         518  CCTCAGC              CCTCAGC(-5/-2)
BciVI         547  GTATCC               GTATCC(6/5)
BcnI         1046  CCGGG                CC^[GC]GG
BseAI         593  TCCGGA               T^CCGGA
BsmAI        1020  GTCTC                GTCTC(1/5)
BsmBI        1019  CGTCTC               CGTCTC(1/5)
BsmI           64  GCATTC               GAATGC(1/-1)
BspEI         593  TCCGGA               T^CCGGA
BssHII       1041  GCGCGC               G^CGCGC
BstEII        744  GGTAACC              G^GT[ACGT]ACC
BstPI         744  GGTAACC              G^GT[ACGT]ACC
BstXI         869  CCAAGGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         590  AGATCC               [GA]^GATC[CT]
Bsu36I        739  CCTGAGG              CC^T[ACGT]AGG
DraIII       1289  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          898  CTCTTC               CTCTTC(1/4)
Eco81I        739  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       744  GGTAACC              G^GT[ACGT]ACC
FseI         1281  GGCCGGCC             GGCCGG^CC
HincII        682  GTCAAC               GT[CT]^[GA]AC
HindII        682  GTCAAC               GT[CT]^[GA]AC
Ksp632I       898  CTCTTC               CTCTTC(1/4)
MfeI          441  CAATTG               C^AATTG
MflI          590  AGATCC               [GA]^GATC[CT]
MroI          593  TCCGGA               T^CCGGA
MseI          565  TTAA                 T^TAA
MunI          441  CAATTG               C^AATTG
NciI         1046  CCGGG                CC^[GC]GG
NcoI          251  CCATGG               C^CATGG
PflFI         493  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1180  CACGTG               CAC^GTG
PmlI         1180  CACGTG               CAC^GTG
PvuII        1480  CAGCTG               CAG^CTG
Tru9I         565  TTAA                 T^TAA
Tth111I       493  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          221  CCAAGCAGAACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         590  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I        454  GGTACC               G^GTACC
              773  GGTACC              
AccII         280  CGCG                 CG^CG
             1042  CGCG                
AcsI          292  GAATTC               [GA]^AATT[CT]
             1003  GAATTC              
AlwI          591  GATCC                GGATC(4/5)
             1118  GGATC               
AlwNI         827  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1240  CAGAACCTG           
ApoI          292  GAATTC               [GA]^AATT[CT]
             1003  GAATTC              
Asp718I       454  GGTACC               G^GTACC
              773  GGTACC              
BbeI           22  GGCGCC               GGCGC^C
             1139  GGCGCC              
BmyI          640  GTGCCC               G[AGT]GC[ACT]^C
             1054  GTGCCC              
BpmI          340  CTGGAG               CTGGAG(16/14)
              346  CTGGAG              
BsaAI        1180  CACGTG               [CT]AC^GT[GA]
             1546  TACGTG              
BsaWI         593  TCCGGA               [AT]^CCGG[AT]
             1448  ACCGGA              
Bsp1286I      640  GTGCCC               G[AGT]GC[ACT]^C
             1054  GTGCCC              
BstUI         280  CGCG                 CG^CG
             1042  CGCG                
EcoRI         292  GAATTC               G^AATTC
             1003  GAATTC              
HaeII          22  GGCGCC               [GA]GCGC^[CT]
             1139  GGCGCC              
KasI           22  GGCGCC               G^GCGCC
             1139  GGCGCC              
KpnI          454  GGTACC               GGTAC^C
              773  GGTACC              
MvnI          280  CGCG                 CG^CG
             1042  CGCG                
NaeI         1142  GCCGGC               GCC^GGC
             1282  GCCGGC              
NarI           22  GGCGCC               GG^CGCC
             1139  GGCGCC              
NgoMIV       1142  GCCGGC               G^CCGGC
             1282  GCCGGC              
PflMI         437  CCACCAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1288  CCACTGGGTGG         
SfoI           22  GGCGCC               GGC^GCC
             1139  GGCGCC              
TaqI          399  TCGA                 T^CGA
              471  TCGA                
TthHB8I       399  TCGA                 T^CGA
              471  TCGA                
Van91I        437  CCACCAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1288  CCACTGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AvaII       
BalI        BanI        BbvI        BsaHI       BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmFI       
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      Tsp509I     TspRI       
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