Restriction map for pF1KB6571
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstUI       
BstXI       Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     FauI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI         1110  CCGC                 CCGC(-3/-1)
AseI         1042  ATTAAT               AT^TAAT
AsnI         1042  ATTAAT               AT^TAAT
BbsI          624  GAAGAC               GAAGAC(2/6)
BbvI          302  GCAGC                GCAGC(8/12)
BclI          273  TGATCA               T^GATCA
BcnI          261  CCGGG                CC^[GC]GG
BpmI          929  CTGGAG               CTGGAG(16/14)
BpuAI         624  GAAGAC               GAAGAC(2/6)
BsaBI         171  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          480  GAGACC               GGTCTC(1/5)
BseRI         251  CTCCTC               GAGGAG(10/8)
BspHI         702  TCATGA               T^CATGA
Bst1107I      555  GTATAC               GTA^TAC
BstAPI          3  GCAAAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       555  GTATAC               GTA^TAC
Cac8I        1006  GCAGGC               GC[ACGT]^[ACGT]GC
DraI          418  TTTAAA               TTT^AAA
DraII        1008  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     1008  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       119  ATGCAT               ATGCA^T
FbaI          273  TGATCA               T^GATCA
Fnu4HI        302  GCAGC                GC^[ACGT]GC
HapII         261  CCGG                 C^CGG
HpaII         261  CCGG                 C^CGG
ItaI          302  GCAGC                GC^[ACGT]GC
MamI          171  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       1110  CCGCTG               C[AC]G^C[GT]G
MspI          261  CCGG                 C^CGG
NciI          261  CCGGG                CC^[GC]GG
NsiI          119  ATGCAT               ATGCA^T
NspI          121  GCATGT               [GA]CATG^[CT]
PflMI         592  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1042  ATTAAT               AT^TAAT
RcaI          702  TCATGA               T^CATGA
SfaNI         839  GATGC                GCATC(5/9)
SfcI          365  CTACAG               C^T[GA][CT]AG
TaqI          488  TCGA                 T^CGA
TseI          302  GCAGC                G^C[AT]GC
TthHB8I       488  TCGA                 T^CGA
Van91I        592  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccI          555  GTATAC               GT^[AC][GT]AC
             1105  GTAGAC              
AlwI          788  GATCC                GGATC(4/5)
               63  GGATC               
AlwNI         368  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              593  CAGGCACTG           
AspHI         820  GAGCAC               G[AT]GC[AT]^C
             1119  GTGCTC              
AvaII          55  GGTCC                G^G[AT]CC
              727  GGACC               
BmyI          820  GAGCAC               G[AGT]GC[ACT]^C
             1119  GTGCTC              
BsaJI         591  CCCAGG               C^C[ACGT][ACGT]GG
              723  CCCAGG              
BsiHKAI       820  GAGCAC               G[AT]GC[AT]^C
             1119  GTGCTC              
Bsp1286I      820  GAGCAC               G[AGT]GC[ACT]^C
             1119  GTGCTC              
BstYI          63  GGATCT               [GA]^GATC[CT]
              787  AGATCC              
HindIII       683  AAGCTT               A^AGCTT
              872  AAGCTT              
MflI           63  GGATCT               [GA]^GATC[CT]
              787  AGATCC              
XhoII          63  GGATCT               [GA]^GATC[CT]
              787  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsiYI       
BslI        BsmAI       BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
FokI        HaeIII      HinfI       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      PleI        RsaI        
Sau3AI      Sau96I      ScrFI       TfiI        Tru9I       
Tsp509I     TspRI       
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