Restriction map for pF1KB0441
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AccI        AccII       AclI        AflII       AflIII      
AgeI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        AspHI       AspI        AviII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfrI        BglII       BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspLU11I    
BspMI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HgaI        HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1107  GACGTC               GACGT^C
Acc65I        227  GGTACC               G^GTACC
AccIII        429  TCCGGA               T^CCGGA
AcyI         1107  GACGTC               G[GA]^CG[CT]C
AlwNI         527  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1063  AGCGCT               AGC^GCT
ApaI           20  GGGCCC               GGGCC^C
Asp700I      1218  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       227  GGTACC               G^GTACC
AvaI          231  CCCGGG               C^[CT]CG[GA]G
AvrII         192  CCTAGG               C^CTAGG
BalI          878  TGGCCA               TGG^CCA
BclI          590  TGATCA               T^GATCA
BlnI          192  CCTAGG               C^CTAGG
BsaHI        1107  GACGTC               G[GA]^CG[CT]C
BsaI          277  GAGACC               GGTCTC(1/5)
BsaWI         429  TCCGGA               [AT]^CCGG[AT]
BseAI         429  TCCGGA               T^CCGGA
BsgI         1264  GTGCAG               GTGCAG(16/14)
BsiEI        1259  CGGCCG               CG[GA][CT]^CG
BsoBI         231  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      380  TGTACA               T^GTACA
BspEI         429  TCCGGA               T^CCGGA
BspHI         458  TCATGA               T^CATGA
BsrFI         125  ACCGGC               [GA]^CCGG[CT]
BsrGI         380  TGTACA               T^GTACA
BstAPI        367  GCAAACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        750  GGTGACC              G^GT[ACGT]ACC
BstPI         750  GGTGACC              G^GT[ACGT]ACC
BstXI         322  CCACAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        125  ACCGGC               [GA]^CCGG[CT]
DraI          853  TTTAAA               TTT^AAA
DraIII        868  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1195  GACACTGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1259  CGGCCG               C^GGCCG
EclXI        1259  CGGCCG               C^GGCCG
Eco47III     1063  AGCGCT               AGC^GCT
Eco52I       1259  CGGCCG               C^GGCCG
EcoO65I       750  GGTGACC              G^GT[ACGT]ACC
FbaI          590  TGATCA               T^GATCA
HaeII        1063  AGCGCT               [GA]GCGC^[CT]
Hin1I        1107  GACGTC               G[GA]^CG[CT]C
KpnI          227  GGTACC               GGTAC^C
MluNI         878  TGGCCA               TGG^CCA
MroI          429  TCCGGA               T^CCGGA
MscI          878  TGGCCA               TGG^CCA
PleI          843  GACTC                GAGTC(4/5)
PpuMI        1093  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          458  TCATGA               T^CATGA
SbfI          300  CCTGCAGG             CCTGCA^GG
SexAI         869  ACCAGGT              A^CC[AT]GGT
SmaI          231  CCCGGG               CCC^GGG
Sse8387I      300  CCTGCAGG             CCTGCA^GG
SspBI         380  TGTACA               T^GTACA
StuI          305  AGGCCT               AGG^CCT
TfiI          502  GAATC                G^A[AT]TC
XmaCI         231  CCCGGG               C^CCGGG
XmaI          231  CCCGGG               C^CCGGG
XmnI         1218  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcsI          162  GAATTT               [GA]^AATT[CT]
              850  GAATTT              
AhdI           37  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              279  GACCCTCTGTC         
ApoI          162  GAATTT               [GA]^AATT[CT]
              850  GAATTT              
AspEI          37  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              279  GACCCTCTGTC         
BanI          227  GGTACC               G^G[CT][GA]CC
              720  GGTGCC              
BbvI          608  GCTGC                GCAGC(8/12)
              782  GCTGC               
BfaI          193  CTAG                 C^TAG
             1012  CTAG                
BpmI          274  CTGGAG               CTGGAG(16/14)
              556  CTGGAG              
BseRI         559  GAGGAG               GAGGAG(10/8)
             1279  GAGGAG              
BsmAI         277  GAGAC                GTCTC(1/5)
             1233  GAGAC               
BstF5I         30  GGATG                GGATG(2/0)
              432  GGATG               
BstYI         966  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              
CfoI          408  GCGC                 GCG^C
             1064  GCGC                
EaeI          878  TGGCCA               [CT]^GGCC[GA]
             1259  CGGCCG              
Eam1105I       37  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              279  GACCCTCTGTC         
EarI          574  CTCTTC               CTCTTC(1/4)
             1303  CTCTTC              
FauI          251  GCGGG                CCCGC(4/6)
              698  GCGGG               
FokI           30  GGATG                GGATG(9/13)
              432  GGATG               
HhaI          408  GCGC                 GCG^C
             1064  GCGC                
HinP1I        408  GCGC                 G^CGC
             1064  GCGC                
HincII         13  GTCAAC               GT[CT]^[GA]AC
              337  GTCAAC              
HindII         13  GTCAAC               GT[CT]^[GA]AC
              337  GTCAAC              
HinfI         502  GAATC                G^A[ACGT]TC
              843  GACTC               
Ksp632I       574  CTCTTC               CTCTTC(1/4)
             1303  CTCTTC              
MaeI          193  CTAG                 C^TAG
             1012  CTAG                
MflI          966  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              
MslI           25  CACAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              739  CACTGCCATG          
NcoI          402  CCATGG               C^CATGG
              450  CCATGG              
PflMI         526  CCAGATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              870  CCAGGTGGTGG         
PvuII         553  CAGCTG               CAG^CTG
              606  CAGCTG              
TseI          608  GCTGC                G^C[AT]GC
              782  GCTGC               
Tsp509I       163  AATT                 ^AATT
              851  AATT                
Van91I        526  CCAGATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              870  CCAGGTGGTGG         
XhoII         966  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BcnI        BglI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        Tru9I       
Tsp45I      TspRI       XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com