Restriction map for pF1KB0441
 (Linear) (Six-base) MAPSORT of: KIBB0441.seq  Check: 8560  from: 1  to: 1332



KIBB0441.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AatII G_ACGT'C

Cuts at:      0    1111    1332 
   Size:       1111     221
 
Acc65I G'GTAC_C

Cuts at:      0     227    1332 
   Size:        227    1105
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     850    1332 
   Size:        850     482
 
AfeI AGC'GCT

Cuts at:      0    1065    1332 
   Size:       1065     267
 
AhdI GACnn_n'nnGTC

Cuts at:      0      42     284    1332 
   Size:         42     242    1048

  Fragments arranged by size: 

                1048     242      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0     532    1332 
   Size:        532     800
 
ApaI G_GGCC'C

Cuts at:      0      24    1332 
   Size:         24    1308
 
ApoI r'AATT_y

Cuts at:      0     162     850    1332 
   Size:        162     688     482

  Fragments arranged by size: 

                 688     482     162
 
AvaI C'yCGr_G

Cuts at:      0     231    1332 
   Size:        231    1101
 
AvrII C'CTAG_G

Cuts at:      0     192    1332 
   Size:        192    1140
 
BanI G'GyrC_C

Cuts at:      0     227     720    1332 
   Size:        227     493     612

  Fragments arranged by size: 

                 612     493     227
 
BanII G_rGCy'C

Cuts at:      0      24     300     894    1332 
   Size:         24     276     594     438

  Fragments arranged by size: 

                 594     438     276      24
 
BclI T'GATC_A

Cuts at:      0     590    1332 
   Size:        590     742
 
BglI GCCn_nnn'nGGC

Cuts at:      0     304     791    1267    1332 
   Size:        304     487     476      65

  Fragments arranged by size: 

                 487     476     304      65
 
Bme1580I G_kGCm'C

Cuts at:      0      24     725    1332 
   Size:         24     701     607

  Fragments arranged by size: 

                 701     607      24
 
BmgBI CAC'GTC

Cuts at:      0     488    1332 
   Size:        488     844
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1070    1332 
   Size:       1070     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     577    1332 
   Size:        295     282     755

  Fragments arranged by size: 

                 755     295     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     271    1332 
   Size:        271    1061
 
BsaHI Gr'CG_yC

Cuts at:      0    1108    1332 
   Size:       1108     224
 
BsaWI w'CCGG_w

Cuts at:      0     429    1332 
   Size:        429     903
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     343     373    1332 
   Size:        343      30     959

  Fragments arranged by size: 

                 959     343      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     574    1294    1332 
   Size:        574     720      38

  Fragments arranged by size: 

                 720     574      38
 
BseYI C'CCAG_C

Cuts at:      0     329     915    1089    1098    1332 
   Size:        329     586     174       9     234

  Fragments arranged by size: 

                 586     329     234     174       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    1332 
   Size:       1285      47
 
BsiEI CG_ry'CG

Cuts at:      0    1262    1332 
   Size:       1262      70
 
Bsp1286I G_dGCh'C

Cuts at:      0      24     300     725     894    1332 
   Size:         24     276     425     169     438

  Fragments arranged by size: 

                 438     425     276     169      24
 
BspEI T'CCGG_A

Cuts at:      0     429    1332 
   Size:        429     903
 
BspHI T'CATG_A

Cuts at:      0     458    1332 
   Size:        458     874
 
BsrFI r'CCGG_y

Cuts at:      0     125    1332 
   Size:        125    1207
 
BsrGI T'GTAC_A

Cuts at:      0     380    1332 
   Size:        380     952
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     373    1332 
   Size:        373     959
 
BstEII G'GTnAC_C

Cuts at:      0     750    1332 
   Size:        750     582
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     329    1332 
   Size:        329    1003
 
BstYI r'GATC_y

Cuts at:      0     966    1184    1332 
   Size:        966     218     148

  Fragments arranged by size: 

                 966     218     148
 
BtgI C'CryG_G

Cuts at:      0     402     450    1113    1332 
   Size:        402      48     663     219

  Fragments arranged by size: 

                 663     402     219      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     579    1332 
   Size:        579     753
 
BtsI GCAGTG_nn'

Cuts at:      0     383     738    1332 
   Size:        383     355     594

  Fragments arranged by size: 

                 594     383     355
 
DraI TTT'AAA

Cuts at:      0     855    1332 
   Size:        855     477
 
DraIII CAC_nnn'GTG

Cuts at:      0     873    1332 
   Size:        873     459
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1201    1332 
   Size:       1201     131
 
EaeI y'GGCC_r

Cuts at:      0     878    1259    1332 
   Size:        878     381      73

  Fragments arranged by size: 

                 878     381      73
 
EagI C'GGCC_G

Cuts at:      0    1259    1332 
   Size:       1259      73
 
EarI CTCTTCn'nnn_

Cuts at:      0     580    1309    1332 
   Size:        580     729      23

  Fragments arranged by size: 

                 729     580      23
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     694    1332 
   Size:        694     638
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     577     850    1332 
   Size:        295     282     273     482

  Fragments arranged by size: 

                 482     295     282     273
 
EcoO109I rG'GnC_Cy

Cuts at:      0      20     189     903    1094    1332 
   Size:         20     169     714     191     238

  Fragments arranged by size: 

                 714     238     191     169      20
 
HaeII r_GCGC'y

Cuts at:      0    1067    1332 
   Size:       1067     265
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     270     302    1332 
   Size:         28      32     210      32    1030

  Fragments arranged by size: 

                1030     210      32      32      28
 
HincII GTy'rAC

Cuts at:      0      15     339    1332 
   Size:         15     324     993

  Fragments arranged by size: 

                 993     324      15
 
KpnI G_GTAC'C

Cuts at:      0     231    1332 
   Size:        231    1101
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     117    1332 
   Size:        117    1215
 
MscI TGG'CCA

Cuts at:      0     880    1332 
   Size:        880     452
 
MslI CAynn'nnrTG

Cuts at:      0      29     743    1332 
   Size:         29     714     589

  Fragments arranged by size: 

                 714     589      29
 
MspA1I CmG'CkG

Cuts at:      0     555     608     698    1332 
   Size:        555      53      90     634

  Fragments arranged by size: 

                 634     555      90      53
 
NcoI C'CATG_G

Cuts at:      0     402     450    1332 
   Size:        402      48     882

  Fragments arranged by size: 

                 882     402      48
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     532     876    1332 
   Size:        532     344     456

  Fragments arranged by size: 

                 532     456     344
 
PfoI T'CCnGG_A

Cuts at:      0    1274    1332 
   Size:       1274      58
 
PpuMI rG'GwC_Cy

Cuts at:      0    1094    1332 
   Size:       1094     238
 
PspOMI G'GGCC_C

Cuts at:      0      20    1332 
   Size:         20    1312
 
PstI C_TGCA'G

Cuts at:      0     305     889    1184    1332 
   Size:        305     584     295     148

  Fragments arranged by size: 

                 584     305     295     148
 
PvuII CAG'CTG

Cuts at:      0     555     608    1332 
   Size:        555      53     724

  Fragments arranged by size: 

                 724     555      53
 
SanDI GG'GwC_CC

Cuts at:      0    1094    1332 
   Size:       1094     238
 
SbfI CC_TGCA'GG

Cuts at:      0     305    1332 
   Size:        305    1027
 
SexAI A'CCwGG_T

Cuts at:      0     869    1332 
   Size:        869     463
 
SfcI C'TryA_G

Cuts at:      0     301     804     885    1180    1332 
   Size:        301     503      81     295     152

  Fragments arranged by size: 

                 503     301     295     152      81
 
SmaI CCC'GGG

Cuts at:      0     233    1332 
   Size:        233    1099
 
StuI AGG'CCT

Cuts at:      0     307    1332 
   Size:        307    1025
 
StyI C'CwwG_G

Cuts at:      0     192     402     450    1332 
   Size:        192     210      48     882

  Fragments arranged by size: 

                 882     210     192      48
 
TatI w'GTAC_w

Cuts at:      0     380    1332 
   Size:        380     952
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     310     342     560     592    1332 
   Size:        310      32     218      32     740

  Fragments arranged by size: 

                 740     310     218      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     244     448    1195    1332 
   Size:        244     204     747     137

  Fragments arranged by size: 

                 747     244     204     137
 
XmaI C'CCGG_G

Cuts at:      0     231    1332 
   Size:        231    1101
 
XmnI GAAnn'nnTTC

Cuts at:      0    1222    1332 
   Size:       1222     110
 
ZraI GAC'GTC

Cuts at:      0    1109    1332 
   Size:       1109     223
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI     AfeI     AhdI    AlwNI     ApaI     ApoI
     AvaI    AvrII     BanI    BanII     BclI     BglI Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI     SbfI
    SexAI     SfcI     SmaI     StuI     StyI     TatI     TstI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI    AflII   AflIII     AgeI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspMI    BsrBI    BsrDI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I     ClaI    CspCI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     XhoI
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