Restriction map for pF1KB6459
 (Linear) (Six-base) MAPSORT of: KIBB6459.seq  Check: 8622  from: 1  to: 954



KIBB6459.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     453     954 
   Size:        453     501
 
AlwNI CAG_nnn'CTG

Cuts at:      0     469     954 
   Size:        469     485
 
ApoI r'AATT_y

Cuts at:      0     350     383     502     768     954 
   Size:        350      33     119     266     186

  Fragments arranged by size: 

                 350     266     186     119      33
 
AseI AT'TA_AT

Cuts at:      0     481     676     954 
   Size:        481     195     278

  Fragments arranged by size: 

                 481     278     195
 
BanII G_rGCy'C

Cuts at:      0     189     954 
   Size:        189     765
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     811     845     954 
   Size:        811      34     109

  Fragments arranged by size: 

                 811     109      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     479     954 
   Size:        479     475
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     856     954 
   Size:         79     777      98

  Fragments arranged by size: 

                 777      98      79
 
BsaBI GATnn'nnATC

Cuts at:      0     908     954 
   Size:        908      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     954 
   Size:        250     704
 
BseYI C'CCAG_C

Cuts at:      0     588     954 
   Size:        588     366
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     954 
   Size:        482     472
 
BsiHKAI G_wGCw'C

Cuts at:      0      77     189     601     954 
   Size:         77     112     412     353

  Fragments arranged by size: 

                 412     353     112      77
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     189     601     954 
   Size:         77     112     412     353

  Fragments arranged by size: 

                 412     353     112      77
 
BspHI T'CATG_A

Cuts at:      0     215     498     954 
   Size:        215     283     456

  Fragments arranged by size: 

                 456     283     215
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     168     954 
   Size:        168     786
 
BssSI C'ACGA_G

Cuts at:      0     230     954 
   Size:        230     724
 
BstYI r'GATC_y

Cuts at:      0     903     954 
   Size:        903      51
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     439     474     954 
   Size:        439      35     480

  Fragments arranged by size: 

                 480     439      35
 
DraI TTT'AAA

Cuts at:      0     854     954 
   Size:        854     100
 
EaeI y'GGCC_r

Cuts at:      0     487     954 
   Size:        487     467
 
EcoICRI GAG'CTC

Cuts at:      0     187     954 
   Size:        187     767
 
EcoRV GAT'ATC

Cuts at:      0     931     954 
   Size:        931      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245     439     471     954 
   Size:        213      32     194      32     483

  Fragments arranged by size: 

                 483     213     194      32      32
 
HindIII A'AGCT_T

Cuts at:      0     178     412     954 
   Size:        178     234     542

  Fragments arranged by size: 

                 542     234     178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     954 
   Size:        611     343
 
MslI CAynn'nnrTG

Cuts at:      0     220     745     954 
   Size:        220     525     209

  Fragments arranged by size: 

                 525     220     209
 
PasI CC'CwG_GG

Cuts at:      0     398     954 
   Size:        398     556
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     790     954 
   Size:        790     164
 
PsiI TTA'TAA

Cuts at:      0     614     954 
   Size:        614     340
 
PstI C_TGCA'G

Cuts at:      0     165     954 
   Size:        165     789
 
SacI G_AGCT'C

Cuts at:      0     189     954 
   Size:        189     765
 
SfcI C'TryA_G

Cuts at:      0     161     954 
   Size:        161     793
 
SmlI C'TyrA_G

Cuts at:      0      94     871     954 
   Size:         94     777      83

  Fragments arranged by size: 

                 777      94      83
 
SspI AAT'ATT

Cuts at:      0     919     954 
   Size:        919      35
 
StuI AGG'CCT

Cuts at:      0     807     954 
   Size:        807     147
 
StyI C'CwwG_G

Cuts at:      0     664     954 
   Size:        664     290
 
TatI w'GTAC_w

Cuts at:      0     371     654     954 
   Size:        371     283     300

  Fragments arranged by size: 

                 371     300     283
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     133     954 
   Size:        133     821
 
XmnI GAAnn'nnTTC

Cuts at:      0     714     954 
   Size:        714     240
 

 Enzymes that do cut:

     AhdI    AlwNI     ApoI     AseI    BanII     BcgI     BmrI    BpuEI
    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BssSI    BstYI    CspCI     DraI     EaeI  EcoICRI    EcoRV    Hin4I
  HindIII     MmeI     MslI     PasI    PflMI     PsiI     PstI     SacI
     SfcI     SmlI     SspI     StuI     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com