Restriction map for
pF1KB0423
(Linear) (Six-base) MAPSORT of: KIBB0423.seq Check: 8993 from: 1 to: 1422
KIBB0423.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 0 135 1422
Size: 135 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1422
Size: 1006 416
AlwNI CAG_nnn'CTG
Cuts at: 0 496 1422
Size: 496 926
ApoI r'AATT_y
Cuts at: 0 33 1422
Size: 33 1389
AvaI C'yCGr_G
Cuts at: 0 299 1422
Size: 299 1123
BamHI G'GATC_C
Cuts at: 0 612 784 1422
Size: 612 172 638
Fragments arranged by size:
638 612 172
BanI G'GyrC_C
Cuts at: 0 197 1422
Size: 197 1225
BanII G_rGCy'C
Cuts at: 0 1244 1422
Size: 1244 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 1267 1422
Size: 443 824 155
Fragments arranged by size:
824 443 155
BfrBI ATG'CAT
Cuts at: 0 751 1422
Size: 751 671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 906 936 1158 1422
Size: 906 30 222 264
Fragments arranged by size:
906 264 222 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 876 1422
Size: 560 316 546
Fragments arranged by size:
560 546 316
Bpu10I CC'TnA_GC
Cuts at: 0 719 1422
Size: 719 703
BsaWI w'CCGG_w
Cuts at: 0 995 1422
Size: 995 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 763 793 888 918 1422
Size: 190 30 543 30 95 30 504
Fragments arranged by size:
543 504 190 95 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 481 912 1422
Size: 119 362 431 510
Fragments arranged by size:
510 431 362 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1422
Size: 881 541
BsiHKAI G_wGCw'C
Cuts at: 0 95 776 1166 1244 1422
Size: 95 681 390 78 178
Fragments arranged by size:
681 390 178 95 78
BsmI GAATG_Cn'
Cuts at: 0 637 1422
Size: 637 785
Bsp1286I G_dGCh'C
Cuts at: 0 95 776 1166 1244 1422
Size: 95 681 390 78 178
Fragments arranged by size:
681 390 178 95 78
BspHI T'CATG_A
Cuts at: 0 306 1422
Size: 306 1116
BstBI TT'CG_AA
Cuts at: 0 77 1422
Size: 77 1345
BstXI CCAn_nnnn'nTGG
Cuts at: 0 284 328 361 667 843 1422
Size: 284 44 33 306 176 579
Fragments arranged by size:
579 306 284 176 44 33
BstYI r'GATC_y
Cuts at: 0 612 652 784 1422
Size: 612 40 132 638
Fragments arranged by size:
638 612 132 40
BtgI C'CryG_G
Cuts at: 0 686 1422
Size: 686 736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 334 1422
Size: 334 1088
BtsI GCAGTG_nn'
Cuts at: 0 1398 1422
Size: 1398 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 238 1422
Size: 238 1184
EarI CTCTTCn'nnn_
Cuts at: 0 300 458 958 1422
Size: 300 158 500 464
Fragments arranged by size:
500 464 300 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1422
Size: 205 1217
EcoICRI GAG'CTC
Cuts at: 0 1242 1422
Size: 1242 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 876 1006 1422
Size: 560 316 130 416
Fragments arranged by size:
560 416 316 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 1422
Size: 1231 191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1317 1422
Size: 1317 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1422
Size: 763 32 627
Fragments arranged by size:
763 627 32
HincII GTy'rAC
Cuts at: 0 136 941 1422
Size: 136 805 481
Fragments arranged by size:
805 481 136
MfeI C'AATT_G
Cuts at: 0 908 1422
Size: 908 514
MslI CAynn'nnrTG
Cuts at: 0 359 665 1100 1422
Size: 359 306 435 322
Fragments arranged by size:
435 359 322 306
MspA1I CmG'CkG
Cuts at: 0 1272 1422
Size: 1272 150
NdeI CA'TA_TG
Cuts at: 0 255 1422
Size: 255 1167
NsiI A_TGCA'T
Cuts at: 0 753 1422
Size: 753 669
PflMI CCAn_nnn'nTGG
Cuts at: 0 496 1422
Size: 496 926
PfoI T'CCnGG_A
Cuts at: 0 376 1422
Size: 376 1046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1302 1334 1422
Size: 1302 32 88
Fragments arranged by size:
1302 88 32
PstI C_TGCA'G
Cuts at: 0 186 1422
Size: 186 1236
PvuII CAG'CTG
Cuts at: 0 1272 1422
Size: 1272 150
SacI G_AGCT'C
Cuts at: 0 1244 1422
Size: 1244 178
SalI G'TCGA_C
Cuts at: 0 134 1422
Size: 134 1288
SapI GCTCTTCn'nnn_
Cuts at: 0 300 1422
Size: 300 1122
SexAI A'CCwGG_T
Cuts at: 0 710 1422
Size: 710 712
SfcI C'TryA_G
Cuts at: 0 182 1118 1422
Size: 182 936 304
Fragments arranged by size:
936 304 182
StyI C'CwwG_G
Cuts at: 0 1336 1422
Size: 1336 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 372 404 888 920 1422
Size: 190 32 150 32 484 32 502
Fragments arranged by size:
502 484 190 150 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1152 1422
Size: 1152 270
XmnI GAAnn'nnTTC
Cuts at: 0 1300 1422
Size: 1300 122
Enzymes that do cut:
AccI AcuI AlwNI ApoI AvaI BamHI BanI BanII
BbsI BfrBI BplI BpmI Bpu10I BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BstBI BstXI BstYI
BtgI BtgZI BtsI DrdI EarI EciI EcoICRI Eco57MI
EcoNI FalI Hin4I HincII MfeI MslI MspA1I NdeI
NsiI PflMI PfoI PpiI PstI PvuII SacI SalI
SapI SexAI SfcI StyI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EaeI EagI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XcmI XhoI XmaI ZraI