Restriction map for pF1KB0419
 (Linear) (Six-base) MAPSORT of: KIBB0419.seq  Check: 1789  from: 1  to: 1416



KIBB0419.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AclI AA'CG_TT

Cuts at:      0     269    1416 
   Size:        269    1147
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587    1416 
   Size:        587     829
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1245    1416 
   Size:       1245     171
 
ApaI G_GGCC'C

Cuts at:      0    1231    1416 
   Size:       1231     185
 
ApoI r'AATT_y

Cuts at:      0     104    1083    1143    1416 
   Size:        104     979      60     273

  Fragments arranged by size: 

                 979     273     104      60
 
BanII G_rGCy'C

Cuts at:      0     120    1231    1416 
   Size:        120    1111     185

  Fragments arranged by size: 

                1111     185     120
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1288    1416 
   Size:       1288     128
 
BclI T'GATC_A

Cuts at:      0     816    1416 
   Size:        816     600
 
Bme1580I G_kGCm'C

Cuts at:      0      13     533    1231    1416 
   Size:         13     520     698     185

  Fragments arranged by size: 

                 698     520     185      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104    1416 
   Size:        104    1312
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     779    1416 
   Size:        779     637
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     462     686    1416 
   Size:        462     224     730

  Fragments arranged by size: 

                 730     462     224
 
BseYI C'CCAG_C

Cuts at:      0    1230    1416 
   Size:       1230     186
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     490    1416 
   Size:        100     390     926

  Fragments arranged by size: 

                 926     390     100
 
BsiEI CG_ry'CG

Cuts at:      0      71    1416 
   Size:         71    1345
 
BsiHKAI G_wGCw'C

Cuts at:      0     856    1416 
   Size:        856     560
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     120     533     856    1231    1416 
   Size:         13     107     413     323     375     185

  Fragments arranged by size: 

                 413     375     323     185     107      13
 
BspHI T'CATG_A

Cuts at:      0    1011    1416 
   Size:       1011     405
 
BsrBI CCG'CTC

Cuts at:      0     933    1416 
   Size:        933     483
 
BsrDI GCAATG_nn'

Cuts at:      0     424    1416 
   Size:        424     992
 
BsrFI r'CCGG_y

Cuts at:      0     429    1416 
   Size:        429     987
 
BsrGI T'GTAC_A

Cuts at:      0     870    1416 
   Size:        870     546
 
BstEII G'GTnAC_C

Cuts at:      0    1300    1416 
   Size:       1300     116
 
BtsI GCAGTG_nn'

Cuts at:      0    1319    1416 
   Size:       1319      97
 
EaeI y'GGCC_r

Cuts at:      0      68     320     605     616    1275    1416 
   Size:         68     252     285      11     659     141

  Fragments arranged by size: 

                 659     285     252     141      68      11
 
EagI C'GGCC_G

Cuts at:      0      68    1416 
   Size:         68    1348
 
EarI CTCTTCn'nnn_

Cuts at:      0     777    1081    1141    1416 
   Size:        777     304      60     275

  Fragments arranged by size: 

                 777     304     275      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587    1416 
   Size:        587     829
 
EcoO109I rG'GnC_Cy

Cuts at:      0     622    1227    1416 
   Size:        622     605     189

  Fragments arranged by size: 

                 622     605     189
 
EcoRI G'AATT_C

Cuts at:      0    1083    1143    1416 
   Size:       1083      60     273

  Fragments arranged by size: 

                1083     273      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     823     855    1416 
   Size:        823      32     561

  Fragments arranged by size: 

                 823     561      32
 
HindIII A'AGCT_T

Cuts at:      0     598    1416 
   Size:        598     818
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     380    1416 
   Size:        380    1036
 
MscI TGG'CCA

Cuts at:      0     607    1277    1416 
   Size:        607     670     139

  Fragments arranged by size: 

                 670     607     139
 
MslI CAynn'nnrTG

Cuts at:      0     242    1016    1064    1124    1416 
   Size:        242     774      48      60     292

  Fragments arranged by size: 

                 774     292     242      60      48
 
MspA1I CmG'CkG

Cuts at:      0     615    1245    1416 
   Size:        615     630     171

  Fragments arranged by size: 

                 630     615     171
 
NspI r_CATG'y

Cuts at:      0     879    1389    1416 
   Size:        879     510      27

  Fragments arranged by size: 

                 879     510      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1188    1416 
   Size:       1188     228
 
PpuMI rG'GwC_Cy

Cuts at:      0     622    1416 
   Size:        622     794
 
PsiI TTA'TAA

Cuts at:      0     917    1416 
   Size:        917     499
 
PspOMI G'GGCC_C

Cuts at:      0    1227    1416 
   Size:       1227     189
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1309    1341    1416 
   Size:       1309      32      75

  Fragments arranged by size: 

                1309      75      32
 
PvuII CAG'CTG

Cuts at:      0    1245    1416 
   Size:       1245     171
 
SfcI C'TryA_G

Cuts at:      0    1088    1148    1329    1416 
   Size:       1088      60     181      87

  Fragments arranged by size: 

                1088     181      87      60
 
SmlI C'TyrA_G

Cuts at:      0     119    1416 
   Size:        119    1297
 
StuI AGG'CCT

Cuts at:      0     463    1416 
   Size:        463     953
 
StyI C'CwwG_G

Cuts at:      0    1305    1416 
   Size:       1305     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     273    1416 
   Size:        273    1143
 
TatI w'GTAC_w

Cuts at:      0     870     948    1416 
   Size:        870      78     468

  Fragments arranged by size: 

                 870     468      78
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      20      52     681     713    1416 
   Size:         20      32     629      32     703

  Fragments arranged by size: 

                 703     629      32      32      20
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1246    1416 
   Size:       1246     170
 
XmnI GAAnn'nnTTC

Cuts at:      0     380    1416 
   Size:        380    1036
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApaI     ApoI    BanII    BciVI     BclI
 Bme1580I    BpuEI     BsaI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BstEII     BtsI
     EaeI     EagI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NspI    PflMI    PpuMI     PsiI
   PspOMI     PsrI    PvuII     SfcI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiWI     BsmI    BsmBI    BspEI    BspMI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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