Restriction map for pF1KB8564
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AclI        AflII       AflIII      AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       BamHI       BbrPI       BbvCI       BcgI        
BclI        BfrI        BsaAI       BsaBI       BsiWI       
BsmI        Bsp1407I    BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstZ17I     CpoI        DraI        
DraIII      Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
MamI        MfeI        MluI        MseI        MunI        
NaeI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Van91I      XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1048  GTCTAC               GT^[AC][GT]AC
AccIII        701  TCCGGA               T^CCGGA
AcsI         1297  GAATTT               [GA]^AATT[CT]
Aor51HI       959  AGCGCT               AGC^GCT
ApoI         1297  GAATTT               [GA]^AATT[CT]
AspI         1945  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         110  TGCGCA               TGC^GCA
AvrII        1282  CCTAGG               C^CTAGG
BalI         2168  TGGCCA               TGG^CCA
BciVI         642  GTATCC               GTATCC(6/5)
BglII        2603  AGATCT               A^GATCT
BlnI         1282  CCTAGG               C^CTAGG
BseAI         701  TCCGGA               T^CCGGA
BsmBI         357  CGTCTC               CGTCTC(1/5)
BspDI         427  ATCGAT               AT^CGAT
BspEI         701  TCCGGA               T^CCGGA
BsrBI         835  GAGCGG               CCGCTC(-3/-3)
BsrDI         603  GCAATG               GCAATG(2/0)
BssHII       2159  GCGCGC               G^CGCGC
BssSI         659  CACGAG               CACGAG(-5/-1)
BstEII        784  GGTGACC              G^GT[ACGT]ACC
BstPI         784  GGTGACC              G^GT[ACGT]ACC
BstYI        2603  AGATCT               [GA]^GATC[CT]
ClaI          427  ATCGAT               AT^CGAT
DrdI          237  GACTTTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2481  CGGCCG               C^GGCCG
EclXI        2481  CGGCCG               C^GGCCG
Eco47III      959  AGCGCT               AGC^GCT
Eco52I       2481  CGGCCG               C^GGCCG
EcoNI          39  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       784  GGTGACC              G^GT[ACGT]ACC
FspI          110  TGCGCA               TGC^GCA
HindIII      1583  AAGCTT               A^AGCTT
KspI          223  CCGCGG               CCGC^GG
MflI         2603  AGATCT               [GA]^GATC[CT]
MluNI        2168  TGGCCA               TGG^CCA
MroI          701  TCCGGA               T^CCGGA
MscI         2168  TGGCCA               TGG^CCA
MslI         1966  CACAATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          915  GCTAGC               G^CTAGC
NspI         1978  ACATGC               [GA]CATG^[CT]
PaeR7I       1733  CTCGAG               C^TCGAG
PflFI        1945  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII         223  CCGCGG               CCGC^GG
SfiI          522  GGCCCAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI         1198  GAATC                G^A[AT]TC
Tth111I      1945  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1733  CTCGAG               C^TCGAG
XhoII        2603  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AatII         355  GACGTC               GACGT^C
             2663  GACGTC              
AlwNI        1115  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1956  CAGCCCCTG           
BglI          523  GCCCAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1789  GCCACACGGGC         
BlpI          369  GCTGAGC              GC^T[ACGT]AGC
             1455  GCTCAGC             
Bpu1102I      369  GCTGAGC              GC^T[ACGT]AGC
             1455  GCTCAGC             
BsaI         1153  GAGACC               GGTCTC(1/5)
             2137  GAGACC              
BsiEI        2481  CGGCCG               CG[GA][CT]^CG
             2583  CGGTCG              
CelII         369  GCTGAGC              GC^T[ACGT]AGC
             1455  GCTCAGC             
NcoI         1313  CCATGG               C^CATGG
             1605  CCATGG              
PvuII           7  CAGCTG               CAG^CTG
             1171  CAGCTG              
SacI         1453  GAGCTC               GAGCT^C
             2633  GAGCTC              
SmlI         1733  CTCGAG               C^T[CT][GA]AG
             2471  CTCAAG              
Tsp509I      1298  AATT                 ^AATT
             1840  AATT                
XcmI          957  CCAGCGCTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1220  CCATCCCCATCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AgeI        AluI        AlwI        AocI        ApaI        
Asp718I     AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PinAI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmaCI       XmaI        
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