Restriction map for pF1KB6440
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsmAI       
BsmBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tsp45I      
Tth111I     XbaI        XcmI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcsI          853  AAATTT               [GA]^AATT[CT]
Alw44I        369  GTGCAC               G^TGCAC
ApaLI         369  GTGCAC               G^TGCAC
ApoI          853  AAATTT               [GA]^AATT[CT]
Asp700I       482  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         390  GAGCCC               G[GA]GC[CT]^C
BbvI          698  GCTGC                GCAGC(8/12)
BpmI            8  CTCCAG               CTGGAG(16/14)
BsaAI         141  TACGTA               [CT]AC^GT[GA]
BseRI         173  GAGGAG               GAGGAG(10/8)
BsgI          561  CTGCAC               GTGCAG(16/14)
BsiWI         282  CGTACG               C^GTACG
Bsp1407I      235  TGTACA               T^GTACA
BspMI         559  ACCTGC               ACCTGC(4/8)
BsrGI         235  TGTACA               T^GTACA
BstNI         127  CCAGG                CC^[AT]GG
Cac8I         152  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          353  GCGC                 GCG^C
DpnII         315  GATC                 ^GATC
DraIII        455  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           59  GACGGACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          133  GAAGAG               CTCTTC(1/4)
EcoNI         199  CCTCTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        127  CCAGG                ^CC[AT]GG
EcoT14I       825  CCAAGG               C^C[AT][AT]GG
EcoT22I      1013  ATGCAT               ATGCA^T
Fnu4HI        698  GCTGC                GC^[ACGT]GC
HaeII         352  GGCGCT               [GA]GCGC^[CT]
HhaI          353  GCGC                 GCG^C
HinP1I        353  GCGC                 G^CGC
HphI          877  GGTGA                GGTGA(8/7)
ItaI          698  GCTGC                GC^[ACGT]GC
Ksp632I       133  GAAGAG               CTCTTC(1/4)
MaeIII        329  GTAAC                ^GT[ACGT]AC
MboI          315  GATC                 ^GATC
MslI          813  CACTGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        476  CAGCTG               C[AC]G^C[GT]G
MvaI          127  CCAGG                CC^[AT]GG
NdeII         315  GATC                 ^GATC
NsiI         1013  ATGCAT               ATGCA^T
NspI          531  ACATGC               [GA]CATG^[CT]
PaeR7I         27  CTCGAG               C^TCGAG
PvuII         476  CAGCTG               CAG^CTG
Sau3AI        315  GATC                 ^GATC
SmlI           27  CTCGAG               C^T[CT][GA]AG
SnaBI         141  TACGTA               TAC^GTA
SspBI         235  TGTACA               T^GTACA
StyI          825  CCAAGG               C^C[AT][AT]GG
TseI          698  GCTGC                G^C[AT]GC
XhoI           27  CTCGAG               C^TCGAG
XmnI          482  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaI           27  CTCGAG               C^[CT]CG[GA]G
              170  CCCGAG              
AvaII          62  GGACC                G^G[AT]CC
              102  GGTCC               
BsaJI         171  CCGAGG               C^C[ACGT][ACGT]GG
              825  CCAAGG              
BsmI          595  GAATGC               GAATGC(1/-1)
              866  GAATGC              
BsoBI          27  CTCGAG               C^[CT]CG[GA]G
              170  CCCGAG              
HaeIII        215  GGCC                 GG^CC
              724  GGCC                
MaeII         142  ACGT                 A^CGT
              445  ACGT                
MseI          109  TTAA                 T^TAA
              857  TTAA                
MwoI          152  GCAAGCCGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              355  GCTGATTCTGC         
PflMI         472  CCATCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              631  CCAGACAATGG         
TaqI           28  TCGA                 T^CGA
              959  TCGA                
Tru9I         109  TTAA                 T^TAA
              857  TTAA                
TthHB8I        28  TCGA                 T^CGA
              959  TCGA                
Van91I        472  CCATCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              631  CCAGACAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        AspHI       BcnI        BmyI        
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      Cfr13I      DdeI        
FokI        HapII       HinfI       HpaII       MboII       
MnlI        MspI        NciI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       SfaNI       TfiI        
Tsp509I     TspRI       
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