Restriction map for pF1KB8545
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbvCI       BclI        BcnI        BfrI        
BglI        BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BstBI       BstUI       Bsu36I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoRI       EcoT22I     
FauI        FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MluI        MroI        MspA1I      MvnI        
NaeI        NciI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmeI        PshAI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Aor51HI      2764  AGCGCT               AGC^GCT
AseI         1534  ATTAAT               AT^TAAT
AsnI         1534  ATTAAT               AT^TAAT
AspHI        1517  GAGCTC               G[AT]GC[AT]^C
BalI         1212  TGGCCA               TGG^CCA
BanII        1517  GAGCTC               G[GA]GC[CT]^C
BbeI         2693  GGCGCC               GGCGC^C
BbrPI        1283  CACGTG               CAC^GTG
BcgI         2709  CGAGAAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1933  AGATCT               A^GATCT
BlpI          526  GCTCAGC              GC^T[ACGT]AGC
BmyI         1517  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      526  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1283  CACGTG               [CT]AC^GT[GA]
BsaI         1322  GGTCTC               GGTCTC(1/5)
BsiHKAI      1517  GAGCTC               G[AT]GC[AT]^C
BsmI          572  GAATGC               GAATGC(1/-1)
Bsp1286I     1517  GAGCTC               G[AGT]GC[ACT]^C
BspHI        1103  TCATGA               T^CATGA
BsrDI        2937  CATTGC               GCAATG(2/0)
BsrFI        2221  GCCGGT               [GA]^CCGG[CT]
BssSI        1736  CACGAG               CACGAG(-5/-1)
Bst1107I     1844  GTATAC               GTA^TAC
BstAPI        697  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1425  GGTTACC              G^GT[ACGT]ACC
BstPI        1425  GGTTACC              G^GT[ACGT]ACC
BstXI        1371  CCAACGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1844  GTATAC               GTA^TAC
CelII         526  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2221  GCCGGT               [GA]^CCGG[CT]
DraI          810  TTTAAA               TTT^AAA
DsaI          516  CCATGG               C^C[GA][CT]GG
EaeI         1212  TGGCCA               [CT]^GGCC[GA]
Eco47III     2764  AGCGCT               AGC^GCT
EcoNI        1885  CCTCAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1425  GGTTACC              G^GT[ACGT]ACC
HgaI            4  GCGTC                GACGC(5/10)
KasI         2693  GGCGCC               G^GCGCC
MfeI          430  CAATTG               C^AATTG
MluNI        1212  TGGCCA               TGG^CCA
MscI         1212  TGGCCA               TGG^CCA
MunI          430  CAATTG               C^AATTG
NarI         2693  GGCGCC               GG^CGCC
NcoI          516  CCATGG               C^CATGG
PmaCI        1283  CACGTG               CAC^GTG
PmlI         1283  CACGTG               CAC^GTG
PpuMI        2228  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1534  ATTAAT               AT^TAAT
RcaI         1103  TCATGA               T^CATGA
SacI         1517  GAGCTC               GAGCT^C
SapI         1565  GAAGAGC              GCTCTTC(1/4)
SfoI         2693  GGCGCC               GGC^GCC
StuI         1893  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AciI         1130  GCGG                 CCGC(-3/-1)
             1320  GCGG                
AcyI            3  GGCGTC               G[GA]^CG[CT]C
             2693  GGCGCC              
AlwNI         529  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1808  CAGAGACTG           
AvaI         2084  CTCGAG               C^[CT]CG[GA]G
             2879  CTCGAG              
BanI         1594  GGCACC               G^G[CT][GA]CC
             2693  GGCGCC              
BbsI         1454  GAAGAC               GAAGAC(2/6)
               62  GTCTTC              
BciVI        2891  GGATAC               GTATCC(6/5)
             2625  GTATCC              
BpuAI        1454  GAAGAC               GAAGAC(2/6)
               62  GTCTTC              
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
             2693  GGCGCC              
BsgI          121  GTGCAG               GTGCAG(16/14)
              461  GTGCAG              
BsoBI        2084  CTCGAG               C^[CT]CG[GA]G
             2879  CTCGAG              
CfoI         2694  GCGC                 GCG^C
             2765  GCGC                
HaeII        2693  GGCGCC               [GA]GCGC^[CT]
             2764  AGCGCT              
HapII        2222  CCGG                 C^CGG
             2748  CCGG                
HhaI         2694  GCGC                 GCG^C
             2765  GCGC                
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
             2693  GGCGCC              
HinP1I       2694  GCGC                 G^CGC
             2765  GCGC                
HpaII        2222  CCGG                 C^CGG
             2748  CCGG                
MaeII         921  ACGT                 A^CGT
             1284  ACGT                
MslI          567  CATACGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2248  CATACTGATG          
MspI         2222  CCGG                 C^CGG
             2748  CCGG                
NheI         1672  GCTAGC               G^CTAGC
             2704  GCTAGC              
PaeR7I       2084  CTCGAG               C^TCGAG
             2879  CTCGAG              
PstI           43  CTGCAG               CTGCA^G
              298  CTGCAG              
SspI         1531  AATATT               AAT^ATT
             1537  AATATT              
Tsp45I        214  GTCAC                ^GT[GC]AC
             1281  GTCAC               
XhoI         2084  CTCGAG               C^TCGAG
             2879  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BfaI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoRV       
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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