Restriction map for pF1KB7994
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AhdI        Aor51HI     AscI        AseI        
AsnI        Asp700I     AspEI       AviII       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MfeI        MluI        
MroI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        476  GGTACC               G^GTACC
AocI          424  CCTGAGG              CC^T[ACGT]AGG
ApaI         1282  GGGCCC               GGGCC^C
Asp718I       476  GGTACC               G^GTACC
AspI          451  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           38  CCCGGG               C^[CT]CG[GA]G
AvaII         497  GGTCC                G^G[AT]CC
BamHI         420  GGATCC               G^GATCC
BanII        1282  GGGCCC               G[GA]GC[CT]^C
BclI          359  TGATCA               T^GATCA
BglI          537  GCCAGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          313  GAGACC               GGTCTC(1/5)
BsoBI          38  CCCGGG               C^[CT]CG[GA]G
BsrBI          75  GAGCGG               CCGCTC(-3/-3)
BstAPI        787  GCAGTCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         420  GGATCC               [GA]^GATC[CT]
Bsu36I        424  CCTGAGG              CC^T[ACGT]AGG
CfoI           64  GCGC                 GCG^C
Eco81I        424  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       670  ATGCAT               ATGCA^T
FauI          528  CCCGC                CCCGC(4/6)
FbaI          359  TGATCA               T^GATCA
HhaI           64  GCGC                 GCG^C
HinP1I         64  GCGC                 G^CGC
KpnI          476  GGTACC               GGTAC^C
MaeIII        790  GTCAC                ^GT[ACGT]AC
MflI          420  GGATCC               [GA]^GATC[CT]
MseI          701  TTAA                 T^TAA
NsiI          670  ATGCAT               ATGCA^T
NspI         1250  ACATGC               [GA]CATG^[CT]
PflFI         451  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          981  GAGTC                GAGTC(4/5)
PstI          303  CTGCAG               CTGCA^G
SfcI          303  CTGCAG               C^T[GA][CT]AG
SmaI           38  CCCGGG               CCC^GGG
Tru9I         701  TTAA                 T^TAA
Tsp45I        790  GTCAC                ^GT[GC]AC
Tth111I       451  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         420  GGATCC               [GA]^GATC[CT]
XmaCI          38  CCCGGG               C^CCGGG
XmaI           38  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          63  CGCG                 CG^CG
             1256  CGCG                
AcsI          334  AAATTC               [GA]^AATT[CT]
              748  GAATTT              
AcyI           12  GGCGTC               G[GA]^CG[CT]C
             1329  GACGCC              
AgeI          494  ACCGGT               A^CCGGT
              574  ACCGGT              
Alw44I        240  GTGCAC               G^TGCAC
              691  GTGCAC              
ApaLI         240  GTGCAC               G^TGCAC
              691  GTGCAC              
ApoI          334  AAATTC               [GA]^AATT[CT]
              748  GAATTT              
AspHI         240  GTGCAC               G[AT]GC[AT]^C
              691  GTGCAC              
BalI          290  TGGCCA               TGG^CCA
              648  TGGCCA              
BsaHI          12  GGCGTC               G[GA]^CG[CT]C
             1329  GACGCC              
BsaWI         494  ACCGGT               [AT]^CCGG[AT]
              574  ACCGGT              
BsiHKAI       240  GTGCAC               G[AT]GC[AT]^C
              691  GTGCAC              
BsmAI         313  GAGAC                GTCTC(1/5)
             1032  GTCTC               
BsmI         1181  GAATGC               GAATGC(1/-1)
              918  GCATTC              
BsrFI         494  ACCGGT               [GA]^CCGG[CT]
              574  ACCGGT              
BstUI          63  CGCG                 CG^CG
             1256  CGCG                
Cfr10I        494  ACCGGT               [GA]^CCGG[CT]
              574  ACCGGT              
DraII        1281  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1282  GGGCCCT             
EcoO109I     1281  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1282  GGGCCCT             
EcoT14I       317  CCAAGG               C^C[AT][AT]GG
              685  CCAAGG              
HgaI         1329  GACGC                GACGC(5/10)
               13  GCGTC               
Hin1I          12  GGCGTC               G[GA]^CG[CT]C
             1329  GACGCC              
MluNI         290  TGGCCA               TGG^CCA
              648  TGGCCA              
MscI          290  TGGCCA               TGG^CCA
              648  TGGCCA              
MvnI           63  CGCG                 CG^CG
             1256  CGCG                
PflMI         156  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              339  CCATGAGCTGG         
PinAI         494  ACCGGT               A^CCGGT
              574  ACCGGT              
StyI          317  CCAAGG               C^C[AT][AT]GG
              685  CCAAGG              
TaqI           73  TCGA                 T^CGA
              999  TCGA                
TthHB8I        73  TCGA                 T^CGA
              999  TCGA                
Van91I        156  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              339  CCATGAGCTGG         
XcmI          278  CCAGTGAGGAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              626  CCAACAATGAACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanI        BbvI        BcnI        BmyI        BpmI        
BsaBI       BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MamI        MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        TseI        
Tsp509I     TspRI       
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