Restriction map for pF1KB7994
 (Linear) (Six-base) MAPSORT of: KIBB7994.seq  Check: 8713  from: 1  to: 1341



KIBB7994.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

Acc65I G'GTAC_C

Cuts at:      0     476    1341 
   Size:        476     865
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     764    1341 
   Size:        757       7     577

  Fragments arranged by size: 

                 757     577       7
 
AgeI A'CCGG_T

Cuts at:      0     494     574    1341 
   Size:        494      80     767

  Fragments arranged by size: 

                 767     494      80
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     941    1341 
   Size:        941     400
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162     793    1043    1092    1341 
   Size:        162     631     250      49     249

  Fragments arranged by size: 

                 631     250     249     162      49
 
ApaI G_GGCC'C

Cuts at:      0    1286    1341 
   Size:       1286      55
 
ApaLI G'TGCA_C

Cuts at:      0     240     691    1341 
   Size:        240     451     650

  Fragments arranged by size: 

                 650     451     240
 
ApoI r'AATT_y

Cuts at:      0     334     748    1341 
   Size:        334     414     593

  Fragments arranged by size: 

                 593     414     334
 
AvaI C'yCGr_G

Cuts at:      0      38    1341 
   Size:         38    1303
 
BamHI G'GATC_C

Cuts at:      0     420    1341 
   Size:        420     921
 
BanI G'GyrC_C

Cuts at:      0     349     476    1105    1341 
   Size:        349     127     629     236

  Fragments arranged by size: 

                 629     349     236     127
 
BanII G_rGCy'C

Cuts at:      0    1286    1341 
   Size:       1286      55
 
BclI T'GATC_A

Cuts at:      0     359    1341 
   Size:        359     982
 
BfrBI ATG'CAT

Cuts at:      0     672    1341 
   Size:        672     669
 
BglI GCCn_nnn'nGGC

Cuts at:      0     543    1341 
   Size:        543     798
 
Bme1580I G_kGCm'C

Cuts at:      0     156     244     352     695    1286    1341 
   Size:        156      88     108     343     591      55

  Fragments arranged by size: 

                 591     343     156     108      88      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     272    1186    1341 
   Size:        272     914     155

  Fragments arranged by size: 

                 914     272     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     331     852    1341 
   Size:        145     186     521     489

  Fragments arranged by size: 

                 521     489     186     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307    1341 
   Size:        307    1034
 
BsaBI GATnn'nnATC

Cuts at:      0     361     451     836    1341 
   Size:        361      90     385     505

  Fragments arranged by size: 

                 505     385     361      90
 
BsaHI Gr'CG_yC

Cuts at:      0      13    1330    1341 
   Size:         13    1317      11

  Fragments arranged by size: 

                1317      13      11
 
BsaWI w'CCGG_w

Cuts at:      0     494     574    1341 
   Size:        494      80     767

  Fragments arranged by size: 

                 767     494      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     130     160     484     514    1341 
   Size:         37      30      63      30     324      30     827

  Fragments arranged by size: 

                 827     324      63      37      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24     219     222     968    1341 
   Size:         24     195       3     746     373

  Fragments arranged by size: 

                 746     373     195      24       3
 
BseYI C'CCAG_C

Cuts at:      0     155     345    1341 
   Size:        155     190     996

  Fragments arranged by size: 

                 996     190     155
 
BsiHKAI G_wGCw'C

Cuts at:      0     244     695    1341 
   Size:        244     451     646

  Fragments arranged by size: 

                 646     451     244
 
BsmI GAATG_Cn'

Cuts at:      0     918    1187    1341 
   Size:        918     269     154

  Fragments arranged by size: 

                 918     269     154
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     244     352     695    1286    1341 
   Size:        156      88     108     343     591      55

  Fragments arranged by size: 

                 591     343     156     108      88      55
 
BsrBI CCG'CTC

Cuts at:      0      77    1341 
   Size:         77    1264
 
BsrDI GCAATG_nn'

Cuts at:      0      56     724    1202    1341 
   Size:         56     668     478     139

  Fragments arranged by size: 

                 668     478     139      56
 
BsrFI r'CCGG_y

Cuts at:      0     494     574    1341 
   Size:        494      80     767

  Fragments arranged by size: 

                 767     494      80
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     793    1341 
   Size:        793     548
 
BstYI r'GATC_y

Cuts at:      0     420    1341 
   Size:        420     921
 
Bsu36I CC'TnA_GG

Cuts at:      0     425    1341 
   Size:        425     916
 
BtsI GCAGTG_nn'

Cuts at:      0     216     791    1341 
   Size:        216     575     550

  Fragments arranged by size: 

                 575     550     216
 
EaeI y'GGCC_r

Cuts at:      0     290     648    1199    1341 
   Size:        290     358     551     142

  Fragments arranged by size: 

                 551     358     290     142
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     331     757     764     852    1341 
   Size:        145     186     426       7      88     489

  Fragments arranged by size: 

                 489     426     186     145      88       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1282    1283    1341 
   Size:       1282       1      58

  Fragments arranged by size: 

                1282      58       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162    1015    1047    1341 
   Size:        130      32     853      32     294

  Fragments arranged by size: 

                 853     294     130      32      32
 
KpnI G_GTAC'C

Cuts at:      0     480    1341 
   Size:        480     861
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1341 
   Size:        415     926
 
MscI TGG'CCA

Cuts at:      0     292     650    1341 
   Size:        292     358     691

  Fragments arranged by size: 

                 691     358     292
 
MslI CAynn'nnrTG

Cuts at:      0     355     400     923    1341 
   Size:        355      45     523     418

  Fragments arranged by size: 

                 523     418     355      45
 
NsiI A_TGCA'T

Cuts at:      0     674    1341 
   Size:        674     667
 
NspI r_CATG'y

Cuts at:      0    1254    1341 
   Size:       1254      87
 
PasI CC'CwG_GG

Cuts at:      0     162    1341 
   Size:        162    1179
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     162     345    1341 
   Size:        162     183     996

  Fragments arranged by size: 

                 996     183     162
 
PfoI T'CCnGG_A

Cuts at:      0     857    1341 
   Size:        857     484
 
PspOMI G'GGCC_C

Cuts at:      0    1282    1341 
   Size:       1282      59
 
PstI C_TGCA'G

Cuts at:      0     307    1341 
   Size:        307    1034
 
SfcI C'TryA_G

Cuts at:      0     303    1341 
   Size:        303    1038
 
SmaI CCC'GGG

Cuts at:      0      40    1341 
   Size:         40    1301
 
StyI C'CwwG_G

Cuts at:      0     317     685    1341 
   Size:        317     368     656

  Fragments arranged by size: 

                 656     368     317
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     154     951    1097    1146    1341 
   Size:        154     797     146      49     195

  Fragments arranged by size: 

                 797     195     154     146      49
 
TatI w'GTAC_w

Cuts at:      0     434    1341 
   Size:        434     907
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516    1072    1104    1341 
   Size:        484      32     556      32     237

  Fragments arranged by size: 

                 556     484     237      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     454    1341 
   Size:        454     887
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285     633    1341 
   Size:        285     348     708

  Fragments arranged by size: 

                 708     348     285
 
XmaI C'CCGG_G

Cuts at:      0      38    1341 
   Size:         38    1303
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BclI    BfrBI     BglI Bme1580I
     BmrI     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI
    BstYI   Bsu36I     BtsI     EaeI  Eco57MI EcoO109I    Hin4I     KpnI
     MmeI     MscI     MslI     NsiI     NspI     PasI    PflMI     PfoI
   PspOMI     PstI     SfcI     SmaI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI     XmnI
     ZraI
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