Restriction map for pF1KB8474
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BcgI        BfrI        BlnI        BsaAI       BsaBI       
BsaHI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspHI       BspLU11I    BspMI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      Cfr10I      
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeIII      MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tsp45I      Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII         59  TCCGGA               T^CCGGA
AhdI          125  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         125  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1714  CTCGAG               C^[CT]CG[GA]G
BbvCI         411  GCTGAGG              CCTCAGC(-5/-2)
BciVI         949  GTATCC               GTATCC(6/5)
BclI          807  TGATCA               T^GATCA
BglII        2021  AGATCT               A^GATCT
BsaWI          59  TCCGGA               [AT]^CCGG[AT]
BseAI          59  TCCGGA               T^CCGGA
BsoBI        1714  CTCGAG               C^[CT]CG[GA]G
BspDI        2275  ATCGAT               AT^CGAT
BspEI          59  TCCGGA               T^CCGGA
BsrDI        1309  GCAATG               GCAATG(2/0)
BssSI         394  CTCGTG               CACGAG(-5/-1)
ClaI         2275  ATCGAT               AT^CGAT
Eam1105I      125  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1764  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          807  TGATCA               T^GATCA
MroI           59  TCCGGA               T^CCGGA
MseI          758  TTAA                 T^TAA
MslI          282  CACACTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI         1305  GCTAGC               G^CTAGC
PaeR7I       1714  CTCGAG               C^TCGAG
PflMI        1562  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2253  GACCTACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         1196  GTCGAC               G^TCGAC
SbfI          459  CCTGCAGG             CCTGCA^GG
Sse8387I      459  CCTGCAGG             CCTGCA^GG
StuI         1929  AGGCCT               AGG^CCT
TfiI         1600  GAATC                G^A[AT]TC
Tru9I         758  TTAA                 T^TAA
Van91I       1562  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          863  CCAATCAGAGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1714  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI         1196  GTCGAC               GT^[AC][GT]AC
             1364  GTCTAC              
Asp700I      1207  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1525  GAAGAACTTC          
BamHI         519  GGATCC               G^GATCC
             1802  GGATCC              
BsaI         2251  GAGACC               GGTCTC(1/5)
             2333  GAGACC              
BsgI          244  CTGCAC               GTGCAG(16/14)
              962  GTGCAG              
EaeI          290  TGGCCG               [CT]^GGCC[GA]
             2095  CGGCCA              
HgaI         1443  GCGTC                GACGC(5/10)
             2324  GCGTC               
HincII       1196  GTCGAC               GT[CT]^[GA]AC
             1242  GTTGAC              
HindII       1196  GTCGAC               GT[CT]^[GA]AC
             1242  GTTGAC              
MaeII         449  ACGT                 A^CGT
             2258  ACGT                
NcoI         1550  CCATGG               C^CATGG
             2213  CCATGG              
NspI          915  ACATGC               [GA]CATG^[CT]
             2390  ACATGC              
SapI         1345  GAAGAGC              GCTCTTC(1/4)
             2235  GAAGAGC             
XmnI         1207  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1525  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrBI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com