Restriction map for pF1KB8474
 (Linear) (Six-base) MAPSORT of: KIBB8474.seq  Check: 6367  from: 1  to: 2523



KIBB8474.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0    1197    1365    2523 
   Size:       1197     168    1158

  Fragments arranged by size: 

                1197    1158     168
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370    1693    1768    1786    1992    2523 
   Size:        370    1323      75      18     206     531

  Fragments arranged by size: 

                1323     531     370     206      75      18
 
AhdI GACnn_n'nnGTC

Cuts at:      0     130    2523 
   Size:        130    2393
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1679    2523 
   Size:       1679     844
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     136     168    2523 
   Size:        136      32    2355

  Fragments arranged by size: 

                2355     136      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51     348     840    2142    2523 
   Size:         51     297     492    1302     381

  Fragments arranged by size: 

                1302     492     381     297      51
 
ApaI G_GGCC'C

Cuts at:      0     392     535     936    2523 
   Size:        392     143     401    1587

  Fragments arranged by size: 

                1587     401     392     143
 
AvaI C'yCGr_G

Cuts at:      0    1714    2523 
   Size:       1714     809
 
BamHI G'GATC_C

Cuts at:      0     519    1802    2523 
   Size:        519    1283     721

  Fragments arranged by size: 

                1283     721     519
 
BanII G_rGCy'C

Cuts at:      0     392     535     646     936    1418    1460    2044    2132 
   Size:        392     143     111     290     482      42     584      88

Cuts at:   2132    2523 
   Size:        391

  Fragments arranged by size: 

                 584     482     392     391     290     143     111      88
                  42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     112    1439    1756    2523 
   Size:        112    1327     317     767

  Fragments arranged by size: 

                1327     767     317     112
 
BbvCI CC'TCA_GC

Cuts at:      0     412    2523 
   Size:        412    2111
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     960    2523 
   Size:        960    1563
 
BclI T'GATC_A

Cuts at:      0     807    2523 
   Size:        807    1716
 
BglI GCCn_nnn'nGGC

Cuts at:      0     823    1928    2094    2523 
   Size:        823    1105     166     429

  Fragments arranged by size: 

                1105     823     429     166
 
BglII A'GATC_T

Cuts at:      0    2021    2523 
   Size:       2021     502
 
BlpI GC'TnA_GC

Cuts at:      0      44     686    2126    2467    2523 
   Size:         44     642    1440     341      56

  Fragments arranged by size: 

                1440     642     341      56      44
 
Bme1580I G_kGCm'C

Cuts at:      0     392     535     936    1401    2523 
   Size:        392     143     401     465    1122

  Fragments arranged by size: 

                1122     465     401     392     143
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     813    1100    2523 
   Size:        813     287    1423

  Fragments arranged by size: 

                1423     813     287
 
BmtI G_CTAG'C

Cuts at:      0    1309    2523 
   Size:       1309    1214
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1977    2523 
   Size:       1977     546
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349    1054    1156    1495    1522    1828    2194    2416 
   Size:        349     705     102     339      27     306     366     222

Cuts at:   2416    2523 
   Size:        107

  Fragments arranged by size: 

                 705     366     349     339     306     222     107     102
                  27
 
Bpu10I CC'TnA_GC

Cuts at:      0     194     412    1538    1926    2523 
   Size:        194     218    1126     388     597

  Fragments arranged by size: 

                1126     597     388     218     194
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1032    1624    1816    1825    1954    2063    2523 
   Size:        874     158     592     192       9     129     109     460

  Fragments arranged by size: 

                 874     592     460     192     158     129     109       9
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2245    2327    2523 
   Size:       2245      82     196

  Fragments arranged by size: 

                2245     196      82
 
BsaWI w'CCGG_w

Cuts at:      0      59    2523 
   Size:         59    2464
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     359     389     739     769    1147    1177    2523 
   Size:        359      30     350      30     378      30    1346

  Fragments arranged by size: 

                1346     378     359     350      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34      37     271     616    1465    1468    1492    1699 
   Size:         34       3     234     345     849       3      24     207

Cuts at:   1699    1702    1744    1951    1992    2191    2413    2523 
   Size:          3      42     207      41     199     222     110

  Fragments arranged by size: 

                 849     345     234     222     207     207     199     110
                  42      41      34      24       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     385    1166    1334    1350    1386    2084    2316    2523 
   Size:        385     781     168      16      36     698     232     207

  Fragments arranged by size: 

                 781     698     385     232     207     168      36      16
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     983    2523 
   Size:        229     754    1540

  Fragments arranged by size: 

                1540     754     229
 
BsiHKAI G_wGCw'C

Cuts at:      0     646    1418    1460    2044    2132    2523 
   Size:        646     772      42     584      88     391

  Fragments arranged by size: 

                 772     646     584     391      88      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     392     535     646     936    1401    1418    1460    2044 
   Size:        392     143     111     290     465      17      42     584

Cuts at:   2044    2132    2523 
   Size:         88     391

  Fragments arranged by size: 

                 584     465     392     391     290     143     111      88
                  42      17
 
BspEI T'CCGG_A

Cuts at:      0      59    2523 
   Size:         59    2464
 
BsrBI CCG'CTC

Cuts at:      0    1734    1863    1925    2523 
   Size:       1734     129      62     598

  Fragments arranged by size: 

                1734     598     129      62
 
BsrDI GCAATG_nn'

Cuts at:      0    1316    2523 
   Size:       1316    1207
 
BssSI C'ACGA_G

Cuts at:      0     394    2523 
   Size:        394    2129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     308     376    2391    2477    2523 
   Size:        308      68    2015      86      46

  Fragments arranged by size: 

                2015     308      86      68      46
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     549     564    1095    2523 
   Size:        549      15     531    1428

  Fragments arranged by size: 

                1428     549     531      15
 
BstYI r'GATC_y

Cuts at:      0     519    1040    1469    1802    2021    2523 
   Size:        519     521     429     333     219     502

  Fragments arranged by size: 

                 521     519     502     429     333     219
 
BtgI C'CryG_G

Cuts at:      0    1461    1550    2213    2523 
   Size:       1461      89     663     310

  Fragments arranged by size: 

                1461     663     310      89
 
BtsI GCAGTG_nn'

Cuts at:      0    1651    1827    2211    2523 
   Size:       1651     176     384     312

  Fragments arranged by size: 

                1651     384     312     176
 
ClaI AT'CG_AT

Cuts at:      0    2276    2523 
   Size:       2276     247
 
EaeI y'GGCC_r

Cuts at:      0     290    2095    2523 
   Size:        290    1805     428

  Fragments arranged by size: 

                1805     428     290
 
EarI CTCTTCn'nnn_

Cuts at:      0     601    1264    1340    2230    2523 
   Size:        601     663      76     890     293

  Fragments arranged by size: 

                 890     663     601     293      76
 
EcoICRI GAG'CTC

Cuts at:      0     644    1416    1458    2042    2130    2523 
   Size:        644     772      42     584      88     393

  Fragments arranged by size: 

                 772     644     584     393      88      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     370    1054    1156    1495    1522    1693    1768 
   Size:        349      21     684     102     339      27     171      75

Cuts at:   1768    1786    1828    1992    2194    2416    2523 
   Size:         18      42     164     202     222     107

  Fragments arranged by size: 

                 684     349     339     222     202     171     164     107
                 102      75      42      27      21      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1768    2523 
   Size:       1768     755
 
EcoO109I rG'GnC_Cy

Cuts at:      0     389     531     933    1134    1836    2060    2223    2485 
   Size:        389     142     402     201     702     224     163     262

Cuts at:   2485    2523 
   Size:         38

  Fragments arranged by size: 

                 702     402     389     262     224     201     163     142
                  38
 
HaeII r_GCGC'y

Cuts at:      0     909    1584    2243    2523 
   Size:        909     675     659     280

  Fragments arranged by size: 

                 909     675     659     280
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27      59     116     148     934     966    1147    1179 
   Size:         27      32      57      32     786      32     181      32

Cuts at:   1179    2523 
   Size:       1344

  Fragments arranged by size: 

                1344     786     181      57      32      32      32      32
                  27
 
HincII GTy'rAC

Cuts at:      0    1198    1244    2523 
   Size:       1198      46    1279

  Fragments arranged by size: 

                1279    1198      46
 
MslI CAynn'nnrTG

Cuts at:      0     286    2523 
   Size:        286    2237
 
MspA1I CmG'CkG

Cuts at:      0      33     327     499     627     894    1102    1851    2139 
   Size:         33     294     172     128     267     208     749     288

Cuts at:   2139    2312    2523 
   Size:        173     211

  Fragments arranged by size: 

                 749     294     288     267     211     208     173     172
                 128      33
 
NcoI C'CATG_G

Cuts at:      0    1550    2213    2523 
   Size:       1550     663     310

  Fragments arranged by size: 

                1550     663     310
 
NheI G'CTAG_C

Cuts at:      0    1305    2523 
   Size:       1305    1218
 
NspI r_CATG'y

Cuts at:      0     919    2394    2523 
   Size:        919    1475     129

  Fragments arranged by size: 

                1475     919     129
 
PasI CC'CwG_GG

Cuts at:      0     526    2523 
   Size:        526    1997
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1568    2523 
   Size:       1568     955
 
PfoI T'CCnGG_A

Cuts at:      0      50    1076    1172    1472    1805    2523 
   Size:         50    1026      96     300     333     718

  Fragments arranged by size: 

                1026     718     333     300      96      50
 
PpuMI rG'GwC_Cy

Cuts at:      0    1134    1836    2060    2223    2523 
   Size:       1134     702     224     163     300

  Fragments arranged by size: 

                1134     702     300     224     163
 
PshAI GACnn'nnGTC

Cuts at:      0    2257    2523 
   Size:       2257     266
 
PspOMI G'GGCC_C

Cuts at:      0     388     531     932    2523 
   Size:        388     143     401    1591

  Fragments arranged by size: 

                1591     401     388     143
 
PspXI vC'TCGA_Gb

Cuts at:      0    1714    2523 
   Size:       1714     809
 
PstI C_TGCA'G

Cuts at:      0     236     313     366     464     674     737     845    1610 
   Size:        236      77      53      98     210      63     108     765

Cuts at:   1610    1886    1898    2387    2523 
   Size:        276      12     489     136

  Fragments arranged by size: 

                 765     489     276     236     210     136     108      98
                  77      63      53      12
 
PvuII CAG'CTG

Cuts at:      0     327     499     627    1102    1851    2523 
   Size:        327     172     128     475     749     672

  Fragments arranged by size: 

                 749     672     475     327     172     128
 
SacI G_AGCT'C

Cuts at:      0     646    1418    1460    2044    2132    2523 
   Size:        646     772      42     584      88     391

  Fragments arranged by size: 

                 772     646     584     391      88      42
 
SalI G'TCGA_C

Cuts at:      0    1196    2523 
   Size:       1196    1327
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1340    2230    2523 
   Size:       1340     890     293

  Fragments arranged by size: 

                1340     890     293
 
SbfI CC_TGCA'GG

Cuts at:      0     464    2523 
   Size:        464    2059
 
SfcI C'TryA_G

Cuts at:      0     232     309     362     418     460     670     733     841 
   Size:        232      77      53      56      42     210      63     108

Cuts at:    841    1606    1693    1882    1894    2383    2523 
   Size:        765      87     189      12     489     140

  Fragments arranged by size: 

                 765     489     232     210     189     140     108      87
                  77      63      56      53      42      12
 
SmlI C'TyrA_G

Cuts at:      0     853    1011    1639    1714    1831    1840    1933    2078 
   Size:        853     158     628      75     117       9      93     145

Cuts at:   2078    2523 
   Size:        445

  Fragments arranged by size: 

                 853     628     445     158     145     117      93      75
                   9
 
StuI AGG'CCT

Cuts at:      0    1931    2523 
   Size:       1931     592
 
StyI C'CwwG_G

Cuts at:      0    1550    1562    2213    2523 
   Size:       1550      12     651     310

  Fragments arranged by size: 

                1550     651     310      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     870    2523 
   Size:        870    1653
 
XhoI C'TCGA_G

Cuts at:      0    1714    2523 
   Size:       1714     809
 
XmnI GAAnn'nnTTC

Cuts at:      0    1211    1529    2523 
   Size:       1211     318     994

  Fragments arranged by size: 

                1211     994     318
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AlfI     AloI    AlwNI     ApaI     AvaI
    BamHI    BanII     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BsrBI    BsrDI    BssSI   BstAPI    BstXI    BstYI     BtgI
     BtsI     ClaI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII     MslI   MspA1I     NcoI     NheI     NspI
     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI     SalI     SapI     SbfI     SfcI     SmlI     StuI
     StyI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BanI     BbeI     BcgI    BfrBI    BmgBI    BsaAI    BsaBI    BsaHI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrFI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI     PsrI
     PvuI    RsrII    SacII    SanDI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XmaI     ZraI
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