Restriction map for pF1KB7886
 (Linear) (Six-base) MAPSORT of: KIBB7886.seq  Check: 8932  from: 1  to: 1953



KIBB7886.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     818    1441    1525    1720    1778    1953 
   Size:        202     616     623      84     195      58     175

  Fragments arranged by size: 

                 623     616     202     195     175      84      58
 
AflIII A'CryG_T

Cuts at:      0      22     553    1953 
   Size:         22     531    1400

  Fragments arranged by size: 

                1400     531      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     514    1464    1608    1953 
   Size:        514     950     144     345

  Fragments arranged by size: 

                 950     514     345     144
 
ApoI r'AATT_y

Cuts at:      0     280     543     631     725    1243    1467    1714    1953 
   Size:        280     263      88      94     518     224     247     239

  Fragments arranged by size: 

                 518     280     263     247     239     224      94      88
            
 
AvaI C'yCGr_G

Cuts at:      0     473    1953 
   Size:        473    1480
 
BanI G'GyrC_C

Cuts at:      0       6    1953 
   Size:          6    1947
 
BanII G_rGCy'C

Cuts at:      0    1918    1953 
   Size:       1918      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1560    1751    1953 
   Size:       1560     191     202

  Fragments arranged by size: 

                1560     202     191
 
BclI T'GATC_A

Cuts at:      0     479    1953 
   Size:        479    1474
 
BfrBI ATG'CAT

Cuts at:      0     772    1953 
   Size:        772    1181
 
BglII A'GATC_T

Cuts at:      0    1278    1649    1953 
   Size:       1278     371     304

  Fragments arranged by size: 

                1278     371     304
 
Bme1580I G_kGCm'C

Cuts at:      0    1841    1953 
   Size:       1841     112
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95    1937    1953 
   Size:         95    1842      16

  Fragments arranged by size: 

                1842      95      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1652    1953 
   Size:        751     901     301

  Fragments arranged by size: 

                 901     751     301
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735     739    1953 
   Size:        735       4    1214

  Fragments arranged by size: 

                1214     735       4
 
BsaBI GATnn'nnATC

Cuts at:      0     530    1028    1045    1861    1953 
   Size:        530     498      17     816      92

  Fragments arranged by size: 

                 816     530     498      92      17
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1628    1658    1953 
   Size:       1628      30     295

  Fragments arranged by size: 

                1628     295      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1649    1953 
   Size:       1649     304
 
BseYI C'CCAG_C

Cuts at:      0      69    1953 
   Size:         69    1884
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1282    1638    1666    1953 
   Size:       1282     356      28     287

  Fragments arranged by size: 

                1282     356     287      28
 
BsiEI CG_ry'CG

Cuts at:      0      44    1953 
   Size:         44    1909
 
BsmI GAATG_Cn'

Cuts at:      0     774    1336    1953 
   Size:        774     562     617

  Fragments arranged by size: 

                 774     617     562
 
Bsp1286I G_dGCh'C

Cuts at:      0    1841    1918    1953 
   Size:       1841      77      35

  Fragments arranged by size: 

                1841      77      35
 
BsrBI CCG'CTC

Cuts at:      0    1427    1476    1953 
   Size:       1427      49     477

  Fragments arranged by size: 

                1427     477      49
 
BsrGI T'GTAC_A

Cuts at:      0    1089    1931    1953 
   Size:       1089     842      22

  Fragments arranged by size: 

                1089     842      22
 
BstBI TT'CG_AA

Cuts at:      0     645    1953 
   Size:        645    1308
 
BstYI r'GATC_y

Cuts at:      0     237    1023    1278    1649    1953 
   Size:        237     786     255     371     304

  Fragments arranged by size: 

                 786     371     304     255     237
 
BtgI C'CryG_G

Cuts at:      0     601     836    1356    1953 
   Size:        601     235     520     597

  Fragments arranged by size: 

                 601     597     520     235
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     509    1358    1953 
   Size:        509     849     595

  Fragments arranged by size: 

                 849     595     509
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     605     640    1892    1910    1927    1945    1953 
   Size:        605      35    1252      18      17      18       8

  Fragments arranged by size: 

                1252     605      35      18      18      17       8
 
EaeI y'GGCC_r

Cuts at:      0      41    1953 
   Size:         41    1912
 
EagI C'GGCC_G

Cuts at:      0      41    1953 
   Size:         41    1912
 
EarI CTCTTCn'nnn_

Cuts at:      0     374    1696    1754    1953 
   Size:        374    1322      58     199

  Fragments arranged by size: 

                1322     374     199      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     751     818    1441    1525    1652    1720    1778 
   Size:        202     549      67     623      84     127      68      58

Cuts at:   1778    1953 
   Size:        175

  Fragments arranged by size: 

                 623     549     202     175     127      84      68      67
                  58
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1173    1953 
   Size:       1173     780
 
EcoRI G'AATT_C

Cuts at:      0     280    1953 
   Size:        280    1673
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     365    1953 
   Size:        365    1588
 
HaeII r_GCGC'y

Cuts at:      0     939    1953 
   Size:        939    1014
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     425     457     906     938    1581    1613    1953 
   Size:        425      32     449      32     643      32     340

  Fragments arranged by size: 

                 643     449     425     340      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1290    1530    1953 
   Size:       1290     240     423

  Fragments arranged by size: 

                1290     423     240
 
HindIII A'AGCT_T

Cuts at:      0    1224    1953 
   Size:       1224     729
 
HpaI GTT'AAC

Cuts at:      0    1290    1530    1953 
   Size:       1290     240     423

  Fragments arranged by size: 

                1290     423     240
 
MluI A'CGCG_T

Cuts at:      0      22    1953 
   Size:         22    1931
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     733     740    1953 
   Size:        733       7    1213

  Fragments arranged by size: 

                1213     733       7
 
MslI CAynn'nnrTG

Cuts at:      0     666     949    1953 
   Size:        666     283    1004

  Fragments arranged by size: 

                1004     666     283
 
MspA1I CmG'CkG

Cuts at:      0     231    1630    1665    1953 
   Size:        231    1399      35     288

  Fragments arranged by size: 

                1399     288     231      35
 
NcoI C'CATG_G

Cuts at:      0    1356    1953 
   Size:       1356     597
 
NotI GC'GGCC_GC

Cuts at:      0      41    1953 
   Size:         41    1912
 
NruI TCG'CGA

Cuts at:      0    1034    1953 
   Size:       1034     919
 
NsiI A_TGCA'T

Cuts at:      0     774    1953 
   Size:        774    1179
 
NspI r_CATG'y

Cuts at:      0     557    1938    1953 
   Size:        557    1381      15

  Fragments arranged by size: 

                1381     557      15
 
PciI A'CATG_T

Cuts at:      0     553    1953 
   Size:        553    1400
 
PpuMI rG'GwC_Cy

Cuts at:      0    1173    1953 
   Size:       1173     780
 
PshAI GACnn'nnGTC

Cuts at:      0     172     554    1953 
   Size:        172     382    1399

  Fragments arranged by size: 

                1399     382     172
 
PstI C_TGCA'G

Cuts at:      0      60    1953 
   Size:         60    1893
 
PvuII CAG'CTG

Cuts at:      0     231    1953 
   Size:        231    1722
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1754    1953 
   Size:       1754     199
 
SfcI C'TryA_G

Cuts at:      0      56     926    1113    1765    1953 
   Size:         56     870     187     652     188

  Fragments arranged by size: 

                 870     652     188     187      56
 
SmlI C'TyrA_G

Cuts at:      0     714     754    1953 
   Size:        714      40    1199

  Fragments arranged by size: 

                1199     714      40
 
StyI C'CwwG_G

Cuts at:      0     349    1356    1953 
   Size:        349    1007     597

  Fragments arranged by size: 

                1007     597     349
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1087    1953 
   Size:       1087     866
 
TatI w'GTAC_w

Cuts at:      0     185     299    1089    1931    1953 
   Size:        185     114     790     842      22

  Fragments arranged by size: 

                 842     790     185     114      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1628    1660    1953 
   Size:       1628      32     293

  Fragments arranged by size: 

                1628     293      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     437    1953 
   Size:        437    1516
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     599    1953 
   Size:        599    1354
 
XmnI GAAnn'nnTTC

Cuts at:      0     632    1899    1953 
   Size:        632    1267      54

  Fragments arranged by size: 

                1267     632      54
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI     BanI    BanII     BbsI
     BclI    BfrBI    BglII Bme1580I     BmrI     BpmI    BpuEI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI Bsp1286I    BsrBI
    BsrGI    BstBI    BstYI     BtgI    BtgZI    CspCI     EaeI     EagI
     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     MluI     MmeI     MslI   MspA1I     NcoI     NotI
     NruI     NsiI     NspI     PciI    PpuMI    PshAI     PstI    PvuII
     SapI     SfcI     SmlI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI     KasI     KpnI     MfeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
     XmaI     ZraI
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