Restriction map for pF1KB5777
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BcnI        BglI        
BlnI        BsaAI       BsaHI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspLU11I    BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraII       DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     FbaI        FseI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeII       MluI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflII         456  CTTAAG               C^TTAAG
AhdI          287  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         287  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        2000  GTGCTC               G[AT]GC[AT]^C
AviII         627  TGCGCA               TGC^GCA
BalI         1467  TGGCCA               TGG^CCA
BcgI          341  GCACGCAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          456  CTTAAG               C^TTAAG
BlpI         2411  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2411  GCTCAGC              GC^T[ACGT]AGC
BsaI         1831  GAGACC               GGTCTC(1/5)
BsiHKAI      2000  GTGCTC               G[AT]GC[AT]^C
BspHI         305  TCATGA               T^CATGA
BspMI         735  GCAGGT               ACCTGC(4/8)
BssSI        1565  CACGAG               CACGAG(-5/-1)
CelII        2411  GCTCAGC              GC^T[ACGT]AGC
DraIII        473  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          561  CCATGG               C^C[GA][CT]GG
EaeI         1467  TGGCCA               [CT]^GGCC[GA]
Eam1105I      287  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2152  GAATTC               G^AATTC
EcoRV        2040  GATATC               GAT^ATC
EcoT14I       561  CCATGG               C^C[AT][AT]GG
EcoT22I      1351  ATGCAT               ATGCA^T
FauI           21  GCGGG                CCCGC(4/6)
FspI          627  TGCGCA               TGC^GCA
HaeII        1370  AGCGCC               [GA]GCGC^[CT]
MfeI         1046  CAATTG               C^AATTG
MluNI        1467  TGGCCA               TGG^CCA
MscI         1467  TGGCCA               TGG^CCA
MunI         1046  CAATTG               C^AATTG
NcoI          561  CCATGG               C^CATGG
NsiI         1351  ATGCAT               ATGCA^T
PleI          917  GAGTC                GAGTC(4/5)
RcaI          305  TCATGA               T^CATGA
SapI         1483  GAAGAGC              GCTCTTC(1/4)
SpeI          838  ACTAGT               A^CTAGT
SphI         1353  GCATGC               GCATG^C
SspI          967  AATATT               AAT^ATT
StuI         2207  AGGCCT               AGG^CCT
StyI          561  CCATGG               C^C[AT][AT]GG
TaqI         1605  TCGA                 T^CGA
TthHB8I      1605  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI        1369  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2006  CAGCTGCTG           
AvaII          35  GGACC                G^G[AT]CC
             2327  GGACC               
BanII         387  GGGCTC               G[GA]GC[CT]^C
             2409  GGGCTC              
BbsI          121  GAAGAC               GAAGAC(2/6)
              999  GAAGAC              
BglII         408  AGATCT               A^GATCT
             2424  AGATCT              
BpmI         1675  CTCCAG               CTGGAG(16/14)
             2003  CTCCAG              
BpuAI         121  GAAGAC               GAAGAC(2/6)
              999  GAAGAC              
BsaBI         292  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              328  GATCTTAATC          
BseRI         390  CTCCTC               GAGGAG(10/8)
              589  CTCCTC              
BsgI          402  GTGCAG               GTGCAG(16/14)
             1795  GTGCAG              
BsmI         1748  GAATGC               GAATGC(1/-1)
             2378  GAATGC              
Bsp1407I     1005  TGTACA               T^GTACA
             1376  TGTACA              
BsrGI        1005  TGTACA               T^GTACA
             1376  TGTACA              
BstYI         408  AGATCT               [GA]^GATC[CT]
             2424  AGATCT              
DraI          666  TTTAAA               TTT^AAA
             1186  TTTAAA              
MamI          292  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              328  GATCTTAATC          
MflI          408  AGATCT               [GA]^GATC[CT]
             2424  AGATCT              
NlaIV         388  GGCTCC               GG[ACGT]^[ACGT]CC
             2326  GGGACC              
PflMI        1241  CCAAACGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1843  CCACCCTCTGG         
SspBI        1005  TGTACA               T^GTACA
             1376  TGTACA              
Van91I       1241  CCAAACGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1843  CCACCCTCTGG         
XcmI          926  CCATAAATATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1874  CCAATGATTTATTGG     
XhoII         408  AGATCT               [GA]^GATC[CT]
             2424  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccIII      AciI        AcsI        AfaI        AluI        
AlwI        ApoI        BbvI        BfaI        BmyI        
BsaJI       BsaWI       BseAI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspEI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MroI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NspI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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