Restriction map for pF1KB5777
 (Linear) (Six-base) MAPSORT of: KIBB5777.seq  Check: 8967  from: 1  to: 2466



KIBB5777.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    2466 
   Size:         83    2383
 
AflII C'TTAA_G

Cuts at:      0     456    2466 
   Size:        456    2010
 
AhdI GACnn_n'nnGTC

Cuts at:      0     292    2466 
   Size:        292    2174
 
AleI CACnn'nnGTG

Cuts at:      0    1048    1558    2466 
   Size:       1048     510     908

  Fragments arranged by size: 

                1048     908     510
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1280    1312    2466 
   Size:       1280      32    1154

  Fragments arranged by size: 

                1280    1154      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1374    2011    2466 
   Size:       1374     637     455

  Fragments arranged by size: 

                1374     637     455
 
ApoI r'AATT_y

Cuts at:      0     232     540     743     761    1183    2152    2466 
   Size:        232     308     203      18     422     969     314

  Fragments arranged by size: 

                 969     422     314     308     232     203      18
 
BanII G_rGCy'C

Cuts at:      0     391    2413    2466 
   Size:        391    2022      53

  Fragments arranged by size: 

                2022     391      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     128    1006    2466 
   Size:        128     878    1460

  Fragments arranged by size: 

                1460     878     128
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     330     364    2466 
   Size:        330      34    2102

  Fragments arranged by size: 

                2102     330      34
 
BfrBI ATG'CAT

Cuts at:      0    1353    2466 
   Size:       1353    1113
 
BglII A'GATC_T

Cuts at:      0     408    2424    2466 
   Size:        408    2016      42

  Fragments arranged by size: 

                2016     408      42
 
BlpI GC'TnA_GC

Cuts at:      0    2412    2466 
   Size:       2412      54
 
Bme1580I G_kGCm'C

Cuts at:      0     650    2466 
   Size:        650    1816
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2177    2466 
   Size:       2177     289
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1660    1988    2466 
   Size:       1660     328     478

  Fragments arranged by size: 

                1660     478     328
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1762    2466 
   Size:        196    1566     704

  Fragments arranged by size: 

                1566     704     196
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1825    2466 
   Size:       1825     641
 
BsaBI GATnn'nnATC

Cuts at:      0     296     332    2466 
   Size:        296      36    2134

  Fragments arranged by size: 

                2134     296      36
 
BsaWI w'CCGG_w

Cuts at:      0      32      41     101     685    2466 
   Size:         32       9      60     584    1781

  Fragments arranged by size: 

                1781     584      60      32       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1986    2016    2466 
   Size:       1986      30     450

  Fragments arranged by size: 

                1986     450      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     580    2466 
   Size:        381     199    1886

  Fragments arranged by size: 

                1886     381     199
 
BseYI C'CCAG_C

Cuts at:      0     174    1977    2045    2310    2466 
   Size:        174    1803      68     265     156

  Fragments arranged by size: 

                1803     265     174     156      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423    1816    2466 
   Size:        423    1393     650

  Fragments arranged by size: 

                1393     650     423
 
BsiHKAI G_wGCw'C

Cuts at:      0    2004    2466 
   Size:       2004     462
 
BsmI GAATG_Cn'

Cuts at:      0    1754    2384    2466 
   Size:       1754     630      82

  Fragments arranged by size: 

                1754     630      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     391     650    2004    2413    2466 
   Size:        391     259    1354     409      53

  Fragments arranged by size: 

                1354     409     391     259      53
 
BspEI T'CCGG_A

Cuts at:      0      32      41     685    2466 
   Size:         32       9     644    1781

  Fragments arranged by size: 

                1781     644      32       9
 
BspHI T'CATG_A

Cuts at:      0     305    2466 
   Size:        305    2161
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     726    2466 
   Size:        726    1740
 
BsrDI GCAATG_nn'

Cuts at:      0     272    1100    2423    2466 
   Size:        272     828    1323      43

  Fragments arranged by size: 

                1323     828     272      43
 
BsrGI T'GTAC_A

Cuts at:      0    1005    1376    2466 
   Size:       1005     371    1090

  Fragments arranged by size: 

                1090    1005     371
 
BssSI C'ACGA_G

Cuts at:      0    1565    2466 
   Size:       1565     901
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     478     820    1198    2373    2466 
   Size:        478     342     378    1175      93

  Fragments arranged by size: 

                1175     478     378     342      93
 
BstYI r'GATC_y

Cuts at:      0     408    2424    2466 
   Size:        408    2016      42

  Fragments arranged by size: 

                2016     408      42
 
BtgI C'CryG_G

Cuts at:      0     561    2466 
   Size:        561    1905
 
BtsI GCAGTG_nn'

Cuts at:      0    1580    2466 
   Size:       1580     886
 
DraI TTT'AAA

Cuts at:      0     668    1188    2466 
   Size:        668     520    1278

  Fragments arranged by size: 

                1278     668     520
 
DraIII CAC_nnn'GTG

Cuts at:      0     478    2466 
   Size:        478    1988
 
EaeI y'GGCC_r

Cuts at:      0    1467    2466 
   Size:       1467     999
 
EarI CTCTTCn'nnn_

Cuts at:      0    1472    1478    2092    2466 
   Size:       1472       6     614     374

  Fragments arranged by size: 

                1472     614     374       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1660    1988    2466 
   Size:         83    1577     328     478

  Fragments arranged by size: 

                1577     478     328      83
 
EcoRI G'AATT_C

Cuts at:      0    2152    2466 
   Size:       2152     314
 
EcoRV GAT'ATC

Cuts at:      0    2042    2466 
   Size:       2042     424
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1171    1405    2157    2466 
   Size:       1171     234     752     309

  Fragments arranged by size: 

                1171     752     309     234
 
FspI TGC'GCA

Cuts at:      0     629    2466 
   Size:        629    1837
 
HaeII r_GCGC'y

Cuts at:      0    1374    2466 
   Size:       1374    1092
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     278     310     883     902     915     934 
   Size:         62      32     184      32     573      19      13      19

Cuts at:    934    1186    1218    2466 
   Size:        252      32    1248

  Fragments arranged by size: 

                1248     573     252     184      62      32      32      32
                  19      19      13
 
HindIII A'AGCT_T

Cuts at:      0     214     640    1594    2389    2466 
   Size:        214     426     954     795      77

  Fragments arranged by size: 

                 954     795     426     214      77
 
MfeI C'AATT_G

Cuts at:      0    1046    2466 
   Size:       1046    1420
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     182     470    1496    1948    2042    2237    2466 
   Size:        121      61     288    1026     452      94     195     229

  Fragments arranged by size: 

                1026     452     288     229     195     121      94      61
            
 
MscI TGG'CCA

Cuts at:      0    1469    2466 
   Size:       1469     997
 
MslI CAynn'nnrTG

Cuts at:      0     135     598    1048    1264    1558    1666    2466 
   Size:        135     463     450     216     294     108     800

  Fragments arranged by size: 

                 800     463     450     294     216     135     108
 
MspA1I CmG'CkG

Cuts at:      0    2008    2277    2373    2466 
   Size:       2008     269      96      93

  Fragments arranged by size: 

                2008     269      96      93
 
NcoI C'CATG_G

Cuts at:      0     561    2466 
   Size:        561    1905
 
NsiI A_TGCA'T

Cuts at:      0    1355    2466 
   Size:       1355    1111
 
NspI r_CATG'y

Cuts at:      0     174     624    1357    2466 
   Size:        174     450     733    1109

  Fragments arranged by size: 

                1109     733     450     174
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1247    1849    2466 
   Size:       1247     602     617

  Fragments arranged by size: 

                1247     617     602
 
PstI C_TGCA'G

Cuts at:      0    1224    2106    2259    2282    2466 
   Size:       1224     882     153      23     184

  Fragments arranged by size: 

                1224     882     184     153      23
 
PvuII CAG'CTG

Cuts at:      0    2008    2277    2373    2466 
   Size:       2008     269      96      93

  Fragments arranged by size: 

                2008     269      96      93
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1478    2466 
   Size:       1478     988
 
SfcI C'TryA_G

Cuts at:      0     581    1220    2102    2255    2278    2466 
   Size:        581     639     882     153      23     188

  Fragments arranged by size: 

                 882     639     581     188     153      23
 
SmlI C'TyrA_G

Cuts at:      0     211     456    1741    2466 
   Size:        211     245    1285     725

  Fragments arranged by size: 

                1285     725     245     211
 
SpeI A'CTAG_T

Cuts at:      0     838    2466 
   Size:        838    1628
 
SphI G_CATG'C

Cuts at:      0    1357    2466 
   Size:       1357    1109
 
SspI AAT'ATT

Cuts at:      0     969    2466 
   Size:        969    1497
 
StuI AGG'CCT

Cuts at:      0    2209    2466 
   Size:       2209     257
 
StyI C'CwwG_G

Cuts at:      0     561    2466 
   Size:        561    1905
 
TatI w'GTAC_w

Cuts at:      0    1005    1026    1376    1615    2466 
   Size:       1005      21     350     239     851

  Fragments arranged by size: 

                1005     851     350     239      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     933    1881    2466 
   Size:        933     948     585

  Fragments arranged by size: 

                 948     933     585
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     AleI     AloI    AlwNI     ApoI    BanII
     BbsI     BcgI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI
    BssSI   BstAPI    BstYI     BtgI     BtsI     DraI   DraIII     EaeI
     EarI  Eco57MI    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI
     NspI    PflMI     PstI    PvuII     SapI     SfcI     SmlI     SpeI
     SphI     SspI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI     BglI
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsiEI    BsiWI
    BsmBI    BsrBI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I     FseI    FspAI   HincII     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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