Restriction map for pF1KB7570
 (Linear) (Six-base) MAPSORT of: KIBB7570.seq  Check: 6590  from: 1  to: 837



KIBB7570.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     516     837 
   Size:        516     321
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680     837 
   Size:        680     157
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     309     837 
   Size:        309     528
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     507     837 
   Size:        288     219     330

  Fragments arranged by size: 

                 330     288     219
 
ApoI r'AATT_y

Cuts at:      0     451     837 
   Size:        451     386
 
AvaI C'yCGr_G

Cuts at:      0     270     837 
   Size:        270     567
 
BanII G_rGCy'C

Cuts at:      0      31     190     707     728     837 
   Size:         31     159     517      21     109

  Fragments arranged by size: 

                 517     159     109      31      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     538     837 
   Size:        538     299
 
BbvCI CC'TCA_GC

Cuts at:      0      19     323     837 
   Size:         19     304     514

  Fragments arranged by size: 

                 514     304      19
 
BclI T'GATC_A

Cuts at:      0     224     837 
   Size:        224     613
 
BlpI GC'TnA_GC

Cuts at:      0      25     837 
   Size:         25     812
 
Bme1580I G_kGCm'C

Cuts at:      0     145     837 
   Size:        145     692
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     108     333     837 
   Size:        108     225     504

  Fragments arranged by size: 

                 504     225     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     721     837 
   Size:        463     258     116

  Fragments arranged by size: 

                 463     258     116
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     323     837 
   Size:         19     304     514

  Fragments arranged by size: 

                 514     304      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      94     321     837 
   Size:         94     227     516

  Fragments arranged by size: 

                 516     227      94
 
BsaAI yAC'GTr

Cuts at:      0     754     837 
   Size:        754      83
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     736     837 
   Size:        205     531     101

  Fragments arranged by size: 

                 531     205     101
 
BsiHKAI G_wGCw'C

Cuts at:      0      31     728     837 
   Size:         31     697     109

  Fragments arranged by size: 

                 697     109      31
 
BsmI GAATG_Cn'

Cuts at:      0     174     837 
   Size:        174     663
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     358     837 
   Size:        358     479
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     145     190     707     728     837 
   Size:         31     114      45     517      21     109

  Fragments arranged by size: 

                 517     114     109      45      31      21
 
BspHI T'CATG_A

Cuts at:      0      74     837 
   Size:         74     763
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     112     516     837 
   Size:        112     404     321

  Fragments arranged by size: 

                 404     321     112
 
BsrBI CCG'CTC

Cuts at:      0     213     837 
   Size:        213     624
 
BstEII G'GTnAC_C

Cuts at:      0     303     837 
   Size:        303     534
 
BstYI r'GATC_y

Cuts at:      0      86     837 
   Size:         86     751
 
BtsI GCAGTG_nn'

Cuts at:      0       3     837 
   Size:          3     834
 
EaeI y'GGCC_r

Cuts at:      0      36     837 
   Size:         36     801
 
EarI CTCTTCn'nnn_

Cuts at:      0     641     656     819     837 
   Size:        641      15     163      18

  Fragments arranged by size: 

                 641     163      18      15
 
EcoICRI GAG'CTC

Cuts at:      0      29     726     837 
   Size:         29     697     111

  Fragments arranged by size: 

                 697     111      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     680     721     837 
   Size:        463     217      41     116

  Fragments arranged by size: 

                 463     217     116      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      94     837 
   Size:         94     743
 
EcoO109I rG'GnC_Cy

Cuts at:      0     327     342     837 
   Size:        327      15     495

  Fragments arranged by size: 

                 495     327      15
 
HaeII r_GCGC'y

Cuts at:      0      63     837 
   Size:         63     774
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     525     557     837 
   Size:        525      32     280

  Fragments arranged by size: 

                 525     280      32
 
HincII GTy'rAC

Cuts at:      0     476     837 
   Size:        476     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     837 
   Size:        470     367
 
MspA1I CmG'CkG

Cuts at:      0     522     699     837 
   Size:        522     177     138

  Fragments arranged by size: 

                 522     177     138
 
PasI CC'CwG_GG

Cuts at:      0     331     684     837 
   Size:        331     353     153

  Fragments arranged by size: 

                 353     331     153
 
PmlI CAC'GTG

Cuts at:      0     754     837 
   Size:        754      83
 
PstI C_TGCA'G

Cuts at:      0     431     527     837 
   Size:        431      96     310

  Fragments arranged by size: 

                 431     310      96
 
PvuII CAG'CTG

Cuts at:      0     522     699     837 
   Size:        522     177     138

  Fragments arranged by size: 

                 522     177     138
 
SacI G_AGCT'C

Cuts at:      0      31     728     837 
   Size:         31     697     109

  Fragments arranged by size: 

                 697     109      31
 
SexAI A'CCwGG_T

Cuts at:      0     254     299     535     837 
   Size:        254      45     236     302

  Fragments arranged by size: 

                 302     254     236      45
 
SfcI C'TryA_G

Cuts at:      0     427     523     837 
   Size:        427      96     314

  Fragments arranged by size: 

                 427     314      96
 
SmaI CCC'GGG

Cuts at:      0     272     837 
   Size:        272     565
 
StyI C'CwwG_G

Cuts at:      0      95     837 
   Size:         95     742
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     612     837 
   Size:        612     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     743     775     837 
   Size:        743      32      62

  Fragments arranged by size: 

                 743      62      32
 
XmaI C'CCGG_G

Cuts at:      0     270     837 
   Size:        270     567
 
XmnI GAAnn'nnTTC

Cuts at:      0     451     837 
   Size:        451     386
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApoI     AvaI    BanII     BbsI
    BbvCI     BclI     BlpI Bme1580I     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI   BstEII    BstYI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI   MspA1I     PasI
     PmlI     PstI    PvuII     SacI    SexAI     SfcI     SmaI     StyI
    TaqII     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     ZraI
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