Restriction map for pF1KB5763
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp700I     AspI        
AviII       AvrII       BamHI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspLU11I    BsrDI       BssHII      BstBI       CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco52I      EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII        471  ACACGT               A^C[GA][CT]GT
AhdI          730  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        621  GTGCAC               G^TGCAC
Aor51HI       997  AGCGCT               AGC^GCT
ApaLI         621  GTGCAC               G^TGCAC
AspEI         730  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          773  GGCGCC               GGCGC^C
BbrPI         472  CACGTG               CAC^GTG
BsaI          175  GAGACC               GGTCTC(1/5)
BsiEI         948  CGACCG               CG[GA][CT]^CG
Bsp1407I      801  TGTACA               T^GTACA
BsrGI         801  TGTACA               T^GTACA
BssSI         611  CTCGTG               CACGAG(-5/-1)
Bst1107I      714  GTATAC               GTA^TAC
BstAPI       1012  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        276  GGTGACC              G^GT[ACGT]ACC
BstPI         276  GGTGACC              G^GT[ACGT]ACC
BstZ17I       714  GTATAC               GTA^TAC
DraI          325  TTTAAA               TTT^AAA
Eam1105I      730  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          781  CTCTTC               CTCTTC(1/4)
Eco47III      997  AGCGCT               AGC^GCT
EcoO65I       276  GGTGACC              G^GT[ACGT]ACC
HgaI         2083  GACGC                GACGC(5/10)
HincII       2128  GTCAAC               GT[CT]^[GA]AC
HindII       2128  GTCAAC               GT[CT]^[GA]AC
KasI          773  GGCGCC               G^GCGCC
Ksp632I       781  CTCTTC               CTCTTC(1/4)
NarI          773  GGCGCC               GG^CGCC
PmaCI         472  CACGTG               CAC^GTG
PmlI          472  CACGTG               CAC^GTG
PvuII        1013  CAGCTG               CAG^CTG
SapI          780  GCTCTTC              GCTCTTC(1/4)
ScaI          644  AGTACT               AGT^ACT
SfoI          773  GGCGCC               GGC^GCC
SmaI           52  CCCGGG               CCC^GGG
SpeI         1674  ACTAGT               A^CTAGT
SphI          977  GCATGC               GCATG^C
SspBI         801  TGTACA               T^GTACA
XmaCI          52  CCCGGG               C^CCGGG
XmaI           52  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I        677  GGTACC               G^GTACC
              835  GGTACC              
AccI          397  GTCTAC               GT^[AC][GT]AC
              714  GTATAC              
AcsI          385  GAATTC               [GA]^AATT[CT]
             2245  GAATTC              
AcyI          773  GGCGCC               G[GA]^CG[CT]C
             2083  GACGCC              
AocI          297  CCTCAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
ApoI          385  GAATTC               [GA]^AATT[CT]
             2245  GAATTC              
Asp718I       677  GGTACC               G^GTACC
              835  GGTACC              
BbsI           22  GAAGAC               GAAGAC(2/6)
             2103  GTCTTC              
BfaI         1226  CTAG                 C^TAG
             1675  CTAG                
BpuAI          22  GAAGAC               GAAGAC(2/6)
             2103  GTCTTC              
BsaAI         400  TACGTG               [CT]AC^GT[GA]
              472  CACGTG              
BsaHI         773  GGCGCC               G[GA]^CG[CT]C
             2083  GACGCC              
BspHI         650  TCATGA               T^CATGA
              821  TCATGA              
Bsu36I        297  CCTCAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
DsaI         1289  CCATGG               C^C[GA][CT]GG
             2352  CCATGG              
Eco81I        297  CCTCAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
EcoRI         385  GAATTC               G^AATTC
             2245  GAATTC              
Hin1I         773  GGCGCC               G[GA]^CG[CT]C
             2083  GACGCC              
KpnI          677  GGTACC               GGTAC^C
              835  GGTACC              
MaeI         1226  CTAG                 C^TAG
             1675  CTAG                
NaeI         1466  GCCGGC               GCC^GGC
             1758  GCCGGC              
NcoI         1289  CCATGG               C^CATGG
             2352  CCATGG              
NgoMIV       1466  GCCGGC               G^CCGGC
             1758  GCCGGC              
NspI          515  ACATGC               [GA]CATG^[CT]
              977  GCATGC              
PpuMI        2168  AGGACCC              [GA]G^G[AT]CC[CT]
             2441  AGGACCC             
PstI         1357  CTGCAG               CTGCA^G
             2383  CTGCAG              
RcaI          650  TCATGA               T^CATGA
              821  TCATGA              
SacI          583  GAGCTC               GAGCT^C
              691  GAGCTC              
TfiI          855  GATTC                G^A[AT]TC
             2305  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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