Restriction map for pF1KB5734
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BbeI        BbrPI       
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsgI        BsiWI       
BsoBI       BspHI       BspMI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MluI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        2027  GACGTC               GACGT^C
AccI          649  GTCTAC               GT^[AC][GT]AC
AocI         1883  CCTTAGG              CC^T[ACGT]AGG
ApaI         2163  GGGCCC               GGGCC^C
AspI         1540  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         383  GGATCC               G^GATCC
BanI          905  GGTGCC               G^G[CT][GA]CC
BbvCI         465  GCTGAGG              CCTCAGC(-5/-2)
BcgI          729  CGACCAGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1742  AGATCT               A^GATCT
BsaBI         864  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         846  CGACCG               CG[GA][CT]^CG
BsmBI        1301  GAGACG               CGTCTC(1/5)
BsmI         2019  GAATGC               GAATGC(1/-1)
Bsp1407I     2119  TGTACA               T^GTACA
BsrBI        2175  CCGCTC               CCGCTC(-3/-3)
BsrGI        2119  TGTACA               T^GTACA
BstBI        1675  TTCGAA               TT^CGAA
Bsu36I       1883  CCTTAGG              CC^T[ACGT]AGG
CfoI          840  GCGC                 GCG^C
DraI         1071  TTTAAA               TTT^AAA
DraII          14  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI          790  GACATTGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           97  CTCTTC               CTCTTC(1/4)
Eco81I       1883  CCTTAGG              CC^T[ACGT]AGG
EcoO109I       14  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         346  GAATTC               G^AATTC
EcoT22I      1813  ATGCAT               ATGCA^T
HaeII         839  GGCGCT               [GA]GCGC^[CT]
HgaI         2112  GACGC                GACGC(5/10)
HhaI          840  GCGC                 GCG^C
HinP1I        840  GCGC                 G^CGC
Ksp632I        97  CTCTTC               CTCTTC(1/4)
MaeII        2028  ACGT                 A^CGT
MamI          864  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1985  CAATTG               C^AATTG
MunI         1985  CAATTG               C^AATTG
NdeI         1486  CATATG               CA^TATG
NsiI         1813  ATGCAT               ATGCA^T
NspV         1675  TTCGAA               TT^CGAA
PflFI        1540  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1826  CCAACCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         847  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          43  CAGCTG               CAG^CTG
SapI           96  GCTCTTC              GCTCTTC(1/4)
SfuI         1675  TTCGAA               TT^CGAA
SphI         1811  GCATGC               GCATG^C
SspBI        2119  TGTACA               T^GTACA
StuI         1880  AGGCCT               AGG^CCT
SwaI         1070  ATTTAAAT             ATTT^AAAT
Tth111I      1540  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1826  CCAACCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1323  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII        866  TCCGGA               T^CCGGA
             1632  TCCGGA              
AcyI         2027  GACGTC               G[GA]^CG[CT]C
             2112  GACGCC              
AflIII        617  ACATGT               A^C[GA][CT]GT
              807  ACATGT              
AlwNI         691  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1438  CAGAATCTG           
BalI            2  TGGCCA               TGG^CCA
             2036  TGGCCA              
BanII        1249  GGGCTC               G[GA]GC[CT]^C
             2163  GGGCCC              
BbsI          334  GAAGAC               GAAGAC(2/6)
             1056  GAAGAC              
BpuAI         334  GAAGAC               GAAGAC(2/6)
             1056  GAAGAC              
BsaHI        2027  GACGTC               G[GA]^CG[CT]C
             2112  GACGCC              
BsaI         1327  GAGACC               GGTCTC(1/5)
             1848  GAGACC              
BseAI         866  TCCGGA               T^CCGGA
             1632  TCCGGA              
BspDI        1493  ATCGAT               AT^CGAT
             1604  ATCGAT              
BspEI         866  TCCGGA               T^CCGGA
             1632  TCCGGA              
BspLU11I      617  ACATGT               A^CATGT
              807  ACATGT              
BstAPI        656  GCAATCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1170  GCAGAGGGTGC         
BstXI        1007  CCAGTGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2153  CCATGCTCATGG        
ClaI         1493  ATCGAT               AT^CGAT
             1604  ATCGAT              
DsaI         1061  CCATGG               C^C[GA][CT]GG
             1854  CCATGG              
EcoRV        1861  GATATC               GAT^ATC
             1918  GATATC              
FauI         2174  CCCGC                CCCGC(4/6)
             1157  GCGGG               
Hin1I        2027  GACGTC               G[GA]^CG[CT]C
             2112  GACGCC              
MluNI           2  TGGCCA               TGG^CCA
             2036  TGGCCA              
MroI          866  TCCGGA               T^CCGGA
             1632  TCCGGA              
MscI            2  TGGCCA               TGG^CCA
             2036  TGGCCA              
NcoI         1061  CCATGG               C^CATGG
             1854  CCATGG              
SfcI          418  CTACAG               C^T[GA][CT]AG
             1892  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com