Restriction map for pF1KB5734
 (Linear) (Six-base) MAPSORT of: KIBB5734.seq  Check: 5924  from: 1  to: 2205



KIBB5734.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AatII G_ACGT'C

Cuts at:      0    2031    2205 
   Size:       2031     174
 
AccI GT'mk_AC

Cuts at:      0     650    2205 
   Size:        650    1555
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     619    1547    2205 
   Size:        463     156     928     658

  Fragments arranged by size: 

                 928     658     463     156
 
AflIII A'CryG_T

Cuts at:      0     617     807    2205 
   Size:        617     190    1398

  Fragments arranged by size: 

                1398     617     190
 
AleI CACnn'nnGTG

Cuts at:      0     559     810    2205 
   Size:        559     251    1395

  Fragments arranged by size: 

                1395     559     251
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1810    2205 
   Size:       1810     395
 
AlwNI CAG_nnn'CTG

Cuts at:      0     696    1443    2205 
   Size:        696     747     762

  Fragments arranged by size: 

                 762     747     696
 
ApaI G_GGCC'C

Cuts at:      0    2167    2205 
   Size:       2167      38
 
ApoI r'AATT_y

Cuts at:      0     346    1074    1678    2205 
   Size:        346     728     604     527

  Fragments arranged by size: 

                 728     604     527     346
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1919    1952    2205 
   Size:       1919      33     253

  Fragments arranged by size: 

                1919     253      33
 
BamHI G'GATC_C

Cuts at:      0     383    2205 
   Size:        383    1822
 
BanI G'GyrC_C

Cuts at:      0     905    2205 
   Size:        905    1300
 
BanII G_rGCy'C

Cuts at:      0    1253    2167    2205 
   Size:       1253     914      38

  Fragments arranged by size: 

                1253     914      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     341    1063    2205 
   Size:        341     722    1142

  Fragments arranged by size: 

                1142     722     341
 
BbvCI CC'TCA_GC

Cuts at:      0     466    2205 
   Size:        466    1739
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     718     752    2205 
   Size:        718      34    1453

  Fragments arranged by size: 

                1453     718      34
 
BfrBI ATG'CAT

Cuts at:      0    1815    2205 
   Size:       1815     390
 
BglII A'GATC_T

Cuts at:      0    1742    2205 
   Size:       1742     463
 
Bme1580I G_kGCm'C

Cuts at:      0    2167    2205 
   Size:       2167      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      23    1001    1537    2205 
   Size:         23     978     536     668

  Fragments arranged by size: 

                 978     668     536      23
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     607    1741    1996    2205 
   Size:        337     270    1134     255     209

  Fragments arranged by size: 

                1134     337     270     255     209
 
Bpu10I CC'TnA_GC

Cuts at:      0     208     466    2205 
   Size:        208     258    1739

  Fragments arranged by size: 

                1739     258     208
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1441    1999    2205 
   Size:        512     929     558     206

  Fragments arranged by size: 

                 929     558     512     206
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1321    1842    2205 
   Size:       1321     521     363

  Fragments arranged by size: 

                1321     521     363
 
BsaBI GATnn'nnATC

Cuts at:      0     868    2205 
   Size:        868    1337
 
BsaHI Gr'CG_yC

Cuts at:      0    2028    2113    2205 
   Size:       2028      85      92

  Fragments arranged by size: 

                2028      92      85
 
BsaWI w'CCGG_w

Cuts at:      0     866    1026    1632    2205 
   Size:        866     160     606     573

  Fragments arranged by size: 

                 866     606     573     160
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     836    1436    1738    2205 
   Size:        836     600     302     467

  Fragments arranged by size: 

                 836     600     467     302
 
BseYI C'CCAG_C

Cuts at:      0      41     547     579    2205 
   Size:         41     506      32    1626

  Fragments arranged by size: 

                1626     506      41      32
 
BsiEI CG_ry'CG

Cuts at:      0     849    2205 
   Size:        849    1356
 
BsiHKAI G_wGCw'C

Cuts at:      0    1084    1181    2047    2205 
   Size:       1084      97     866     158

  Fragments arranged by size: 

                1084     866     158      97
 
BsmI GAATG_Cn'

Cuts at:      0    2025    2205 
   Size:       2025     180
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1295    2205 
   Size:       1295     910
 
Bsp1286I G_dGCh'C

Cuts at:      0    1084    1181    1253    2047    2167    2205 
   Size:       1084      97      72     794     120      38

  Fragments arranged by size: 

                1084     794     120      97      72      38
 
BspEI T'CCGG_A

Cuts at:      0     866    1632    2205 
   Size:        866     766     573

  Fragments arranged by size: 

                 866     766     573
 
BsrBI CCG'CTC

Cuts at:      0    2177    2205 
   Size:       2177      28
 
BsrGI T'GTAC_A

Cuts at:      0    2119    2205 
   Size:       2119      86
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     662    1176    2205 
   Size:        662     514    1029

  Fragments arranged by size: 

                1029     662     514
 
BstBI TT'CG_AA

Cuts at:      0    1676    2205 
   Size:       1676     529
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1014    2160    2205 
   Size:       1014    1146      45

  Fragments arranged by size: 

                1146    1014      45
 
BstYI r'GATC_y

Cuts at:      0     383     863     869     917    1451    1742    1761    2205 
   Size:        383     480       6      48     534     291      19     444

  Fragments arranged by size: 

                 534     480     444     383     291      48      19       6
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1884    2205 
   Size:       1884     321
 
BtgI C'CryG_G

Cuts at:      0    1061    1854    2205 
   Size:       1061     793     351

  Fragments arranged by size: 

                1061     793     351
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1773    2205 
   Size:       1773     432
 
BtsI GCAGTG_nn'

Cuts at:      0     243     519     565    1799    2205 
   Size:        243     276      46    1234     406

  Fragments arranged by size: 

                1234     406     276     243      46
 
ClaI AT'CG_AT

Cuts at:      0    1494    1605    2205 
   Size:       1494     111     600

  Fragments arranged by size: 

                1494     600     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     257     292     517     552    2205 
   Size:        257      35     225      35    1653

  Fragments arranged by size: 

                1653     257     225      35      35
 
DraI TTT'AAA

Cuts at:      0    1073    2205 
   Size:       1073    1132
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     796    2205 
   Size:        796    1409
 
EaeI y'GGCC_r

Cuts at:      0       2     328    2036    2205 
   Size:          2     326    1708     169

  Fragments arranged by size: 

                1708     326     169       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     103    2205 
   Size:        103    2102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     463     607     619    1547    1741    1996    2205 
   Size:        337     126     144      12     928     194     255     209

  Fragments arranged by size: 

                 928     337     255     209     194     144     126      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     316    1228    1244    2205 
   Size:        316     912      16     961

  Fragments arranged by size: 

                 961     912     316      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15    2205 
   Size:         15    2190
 
EcoRI G'AATT_C

Cuts at:      0     346    2205 
   Size:        346    1859
 
EcoRV GAT'ATC

Cuts at:      0    1863    1920    2205 
   Size:       1863      57     285

  Fragments arranged by size: 

                1863     285      57
 
HaeII r_GCGC'y

Cuts at:      0     843    2205 
   Size:        843    1362
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1483    1515    1598    1630    2205 
   Size:       1483      32      83      32     575

  Fragments arranged by size: 

                1483     575      83      32      32
 
MfeI C'AATT_G

Cuts at:      0    1985    2205 
   Size:       1985     220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1850    2205 
   Size:       1850     355
 
MscI TGG'CCA

Cuts at:      0       4    2038    2205 
   Size:          4    2034     167

  Fragments arranged by size: 

                2034     167       4
 
MslI CAynn'nnrTG

Cuts at:      0     559     810     953    1792    2158    2205 
   Size:        559     251     143     839     366      47

  Fragments arranged by size: 

                 839     559     366     251     143      47
 
MspA1I CmG'CkG

Cuts at:      0      45     851    1758    2205 
   Size:         45     806     907     447

  Fragments arranged by size: 

                 907     806     447      45
 
NcoI C'CATG_G

Cuts at:      0    1061    1854    2205 
   Size:       1061     793     351

  Fragments arranged by size: 

                1061     793     351
 
NdeI CA'TA_TG

Cuts at:      0    1487    2205 
   Size:       1487     718
 
NsiI A_TGCA'T

Cuts at:      0    1817    2205 
   Size:       1817     388
 
NspI r_CATG'y

Cuts at:      0     621     811    1712    1815    2205 
   Size:        621     190     901     103     390

  Fragments arranged by size: 

                 901     621     390     190     103
 
PasI CC'CwG_GG

Cuts at:      0    1380    2205 
   Size:       1380     825
 
PciI A'CATG_T

Cuts at:      0     617     807    2205 
   Size:        617     190    1398

  Fragments arranged by size: 

                1398     617     190
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1832    2205 
   Size:       1832     373
 
PfoI T'CCnGG_A

Cuts at:      0     386     971    2205 
   Size:        386     585    1234

  Fragments arranged by size: 

                1234     585     386
 
PshAI GACnn'nnGTC

Cuts at:      0     851    2205 
   Size:        851    1354
 
PspOMI G'GGCC_C

Cuts at:      0    2163    2205 
   Size:       2163      42
 
PvuII CAG'CTG

Cuts at:      0      45    2205 
   Size:         45    2160
 
SapI GCTCTTCn'nnn_

Cuts at:      0     103    2205 
   Size:        103    2102
 
SfcI C'TryA_G

Cuts at:      0     418    1892    2205 
   Size:        418    1474     313

  Fragments arranged by size: 

                1474     418     313
 
SmlI C'TyrA_G

Cuts at:      0     527    1456    2014    2199    2205 
   Size:        527     929     558     185       6

  Fragments arranged by size: 

                 929     558     527     185       6
 
SphI G_CATG'C

Cuts at:      0    1815    2205 
   Size:       1815     390
 
StuI AGG'CCT

Cuts at:      0    1882    2205 
   Size:       1882     323
 
StyI C'CwwG_G

Cuts at:      0       5     129    1061    1854    2205 
   Size:          5     124     932     793     351

  Fragments arranged by size: 

                 932     793     351     124       5
 
SwaI ATTT'AAAT

Cuts at:      0    1073    2205 
   Size:       1073    1132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     804    1135    1139    2149    2205 
   Size:        804     331       4    1010      56

  Fragments arranged by size: 

                1010     804     331      56       4
 
TatI w'GTAC_w

Cuts at:      0     716    2119    2205 
   Size:        716    1403      86

  Fragments arranged by size: 

                1403     716      86
 
Tth111I GACn'n_nGTC

Cuts at:      0    1543    2205 
   Size:       1543     662
 
XbaI T'CTAG_A

Cuts at:      0    1323    2205 
   Size:       1323     882
 
ZraI GAC'GTC

Cuts at:      0    2029    2205 
   Size:       2029     176
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
     ApoI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BfrBI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrGI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI     PasI     PciI    PflMI     PfoI    PshAI
   PspOMI    PvuII     SapI     SfcI     SmlI     SphI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BbeI    BciVI
     BclI     BglI     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaXI
     BsgI    BsiWI    BspHI    BspMI    BsrDI    BsrFI   BssHII    BssSI
   BstEII  BstZ17I   DraIII     EagI     EciI  EcoICRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PmeI
     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     TstI     XcmI     XhoI     XmaI     XmnI
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