Restriction map for pF1KB5710
 (Linear) (Six-base) MAPSORT of: KIBB5710.seq  Check: 3339  from: 1  to: 2151



KIBB5710.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     978    1092    2151 
   Size:        978     114    1059

  Fragments arranged by size: 

                1059     978     114
 
Acc65I G'GTAC_C

Cuts at:      0     685    2151 
   Size:        685    1466
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     235     328     695     741     898    1375    1613 
   Size:        220      15      93     367      46     157     477     238

Cuts at:   1613    2151 
   Size:        538

  Fragments arranged by size: 

                 538     477     367     238     220     157      93      46
                  15
 
AfeI AGC'GCT

Cuts at:      0     577    2151 
   Size:        577    1574
 
AhdI GACnn_n'nnGTC

Cuts at:      0     483     516    2151 
   Size:        483      33    1635

  Fragments arranged by size: 

                1635     483      33
 
AleI CACnn'nnGTG

Cuts at:      0    1330    2151 
   Size:       1330     821
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     659    1840    2029    2151 
   Size:        659    1181     189     122

  Fragments arranged by size: 

                1181     659     189     122
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1895    1927    2151 
   Size:       1895      32     224

  Fragments arranged by size: 

                1895     224      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     630     846     876    1113    1991    2079    2151 
   Size:         90     540     216      30     237     878      88      72

  Fragments arranged by size: 

                 878     540     237     216      90      88      72      30
            
 
ApaI G_GGCC'C

Cuts at:      0    1545    2151 
   Size:       1545     606
 
BamHI G'GATC_C

Cuts at:      0    1314    2151 
   Size:       1314     837
 
BanI G'GyrC_C

Cuts at:      0     685     953    1863    2040    2151 
   Size:        685     268     910     177     111

  Fragments arranged by size: 

                 910     685     268     177     111
 
BanII G_rGCy'C

Cuts at:      0     332     458    1069    1370    1545    2151 
   Size:        332     126     611     301     175     606

  Fragments arranged by size: 

                 611     606     332     301     175     126
 
BbeI G_GCGC'C

Cuts at:      0     957    2044    2151 
   Size:        957    1087     107

  Fragments arranged by size: 

                1087     957     107
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     899    2151 
   Size:        899    1252
 
BbvCI CC'TCA_GC

Cuts at:      0     551     970    2151 
   Size:        551     419    1181

  Fragments arranged by size: 

                1181     551     419
 
BglI GCCn_nnn'nGGC

Cuts at:      0     865     962    1064    1144    2151 
   Size:        865      97     102      80    1007

  Fragments arranged by size: 

                1007     865     102      97      80
 
BglII A'GATC_T

Cuts at:      0    1653    2151 
   Size:       1653     498
 
BlpI GC'TnA_GC

Cuts at:      0      17    2151 
   Size:         17    2134
 
Bme1580I G_kGCm'C

Cuts at:      0    1545    1866    2151 
   Size:       1545     321     285

  Fragments arranged by size: 

                1545     321     285
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2049    2151 
   Size:       2049     102
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1251    2151 
   Size:       1251     900
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     610     754     784     826     949    1021    1063 
   Size:        145     465     144      30      42     123      72      42

Cuts at:   1063    1105    1198    1417    1486    1642    1678    1714    1853 
   Size:         42      93     219      69     156      36      36     139

Cuts at:   1853    1948    2151 
   Size:         95     203

  Fragments arranged by size: 

                 465     219     203     156     145     144     139     123
                  95      93      72      69      42      42      42      36
                  36      30
 
Bpu10I CC'TnA_GC

Cuts at:      0      91     551     970    1369    2151 
   Size:         91     460     419     399     782

  Fragments arranged by size: 

                 782     460     419     399      91
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      35     700    2151 
   Size:         35     665    1451

  Fragments arranged by size: 

                1451     665      35
 
BsaAI yAC'GTr

Cuts at:      0    1803    2151 
   Size:       1803     348
 
BsaHI Gr'CG_yC

Cuts at:      0     954    2041    2112    2151 
   Size:        954    1087      71      39

  Fragments arranged by size: 

                1087     954      71      39
 
BsaWI w'CCGG_w

Cuts at:      0     833    2151 
   Size:        833    1318
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1171    1201    1204    1234    2151 
   Size:       1171      30       3      30     917

  Fragments arranged by size: 

                1171     917      30      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     559     568     631     763    1147    1270    1462    1702 
   Size:        559       9      63     132     384     123     192     240

Cuts at:   1702    1747    1798    2047    2151 
   Size:         45      51     249     104

  Fragments arranged by size: 

                 559     384     249     240     192     132     123     104
                  63      51      45       9
 
BseYI C'CCAG_C

Cuts at:      0     354     728     919    1079    1271    2026    2151 
   Size:        354     374     191     160     192     755     125

  Fragments arranged by size: 

                 755     374     354     192     191     160     125
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1126    1434    2151 
   Size:       1126     308     717

  Fragments arranged by size: 

                1126     717     308
 
BsiEI CG_ry'CG

Cuts at:      0    1430    1434    2151 
   Size:       1430       4     717

  Fragments arranged by size: 

                1430     717       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     458    1370    2151 
   Size:        332     126     912     781

  Fragments arranged by size: 

                 912     781     332     126
 
BsmI GAATG_Cn'

Cuts at:      0     648    1171    2151 
   Size:        648     523     980

  Fragments arranged by size: 

                 980     648     523
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      71     487    1369    1492    2151 
   Size:         71     416     882     123     659

  Fragments arranged by size: 

                 882     659     416     123      71
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     458    1069    1370    1545    1866    2151 
   Size:        332     126     611     301     175     321     285

  Fragments arranged by size: 

                 611     332     321     301     285     175     126
 
BspHI T'CATG_A

Cuts at:      0     404     458    1644    2151 
   Size:        404      54    1186     507

  Fragments arranged by size: 

                1186     507     404      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     515     978    1092    1565    2151 
   Size:        515     463     114     473     586

  Fragments arranged by size: 

                 586     515     473     463     114
 
BsrBI CCG'CTC

Cuts at:      0     594     660    1063    1215    1230    1440    2037    2151 
   Size:        594      66     403     152      15     210     597     114

  Fragments arranged by size: 

                 597     594     403     210     152     114      66      15
            
 
BsrFI r'CCGG_y

Cuts at:      0     668     950    1015    1508    2151 
   Size:        668     282      65     493     643

  Fragments arranged by size: 

                 668     643     493     282      65
 
BssHII G'CGCG_C

Cuts at:      0    1074    1481    1483    2151 
   Size:       1074     407       2     668

  Fragments arranged by size: 

                1074     668     407       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     603    1113    1749    2151 
   Size:        603     510     636     402

  Fragments arranged by size: 

                 636     603     510     402
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1332    2151 
   Size:       1332     819
 
BstYI r'GATC_y

Cuts at:      0     177     585    1314    1352    1653    2151 
   Size:        177     408     729      38     301     498

  Fragments arranged by size: 

                 729     498     408     301     177      38
 
Bsu36I CC'TnA_GG

Cuts at:      0      83    1885    2151 
   Size:         83    1802     266

  Fragments arranged by size: 

                1802     266      83
 
BtgI C'CryG_G

Cuts at:      0    1282    1331    1488    1778    1832    2097    2151 
   Size:       1282      49     157     290      54     265      54

  Fragments arranged by size: 

                1282     290     265     157      54      54      49
 
BtsI GCAGTG_nn'

Cuts at:      0     698    2151 
   Size:        698    1453
 
EaeI y'GGCC_r

Cuts at:      0    1017    1427    1849    2151 
   Size:       1017     410     422     302

  Fragments arranged by size: 

                1017     422     410     302
 
EagI C'GGCC_G

Cuts at:      0    1427    2151 
   Size:       1427     724
 
EarI CTCTTCn'nnn_

Cuts at:      0     272    2151 
   Size:        272    1879
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1240    1740    2151 
   Size:       1240     500     411

  Fragments arranged by size: 

                1240     500     411
 
EcoICRI GAG'CTC

Cuts at:      0     330     456    1368    2151 
   Size:        330     126     912     783

  Fragments arranged by size: 

                 912     783     330     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     220     235     328     610     695     741     754 
   Size:        145      75      15      93     282      85      46      13

Cuts at:    754     784     826     898     949    1021    1063    1105    1198 
   Size:         30      42      72      51      72      42      42      93

Cuts at:   1198    1375    1417    1486    1613    1642    1678    1714    1853 
   Size:        177      42      69     127      29      36      36     139

Cuts at:   1853    1948    2151 
   Size:         95     203

  Fragments arranged by size: 

                 282     203     177     145     139     127      95      93
                  93      85      75      72      72      69      51      46
                  42      42      42      42      36      36      30      29
                  15      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     541     613     859    2151 
   Size:        541      72     246    1292

  Fragments arranged by size: 

                1292     541     246      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     184     873    1554    2151 
   Size:         87      97     689     681     597

  Fragments arranged by size: 

                 689     681     597      97      87
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     240    2151 
   Size:        240    1911
 
FseI GG_CCGG'CC

Cuts at:      0     672    2151 
   Size:        672    1479
 
HaeII r_GCGC'y

Cuts at:      0     579     789     847     957    1461    2044    2151 
   Size:        579     210      58     110     504     583     107

  Fragments arranged by size: 

                 583     579     504     210     110     107      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     502     534    1306    1338    2151 
   Size:        469      32       1      32     772      32     813

  Fragments arranged by size: 

                 813     772     469      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0    1292    2151 
   Size:       1292     859
 
HindIII A'AGCT_T

Cuts at:      0    1639    2151 
   Size:       1639     512
 
KasI G'GCGC_C

Cuts at:      0     953    2040    2151 
   Size:        953    1087     111

  Fragments arranged by size: 

                1087     953     111
 
KpnI G_GTAC'C

Cuts at:      0     689    2151 
   Size:        689    1462
 
MfeI C'AATT_G

Cuts at:      0     739    2151 
   Size:        739    1412
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1475    1944    2151 
   Size:       1475     469     207

  Fragments arranged by size: 

                1475     469     207
 
MslI CAynn'nnrTG

Cuts at:      0    1330    1822    2151 
   Size:       1330     492     329

  Fragments arranged by size: 

                1330     492     329
 
MspA1I CmG'CkG

Cuts at:      0      98     348     600     732     927     999    1083    1095 
   Size:         98     250     252     132     195      72      84      12

Cuts at:   1095    1125    1275    1284    1395    1848    1855    1959    2076 
   Size:         30     150       9     111     453       7     104     117

Cuts at:   2076    2151 
   Size:         75

  Fragments arranged by size: 

                 453     252     250     195     150     132     117     111
                 104      98      84      75      72      30      12       9
                   7
 
NaeI GCC'GGC

Cuts at:      0     670     952    1017    2151 
   Size:        670     282      65    1134

  Fragments arranged by size: 

                1134     670     282      65
 
NarI GG'CG_CC

Cuts at:      0     954    2041    2151 
   Size:        954    1087     110

  Fragments arranged by size: 

                1087     954     110
 
NcoI C'CATG_G

Cuts at:      0    1778    1832    2151 
   Size:       1778      54     319

  Fragments arranged by size: 

                1778     319      54
 
NgoMIV G'CCGG_C

Cuts at:      0     668     950    1015    2151 
   Size:        668     282      65    1136

  Fragments arranged by size: 

                1136     668     282      65
 
NspI r_CATG'y

Cuts at:      0     433    2151 
   Size:        433    1718
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1494    2151 
   Size:       1494     657
 
PpuMI rG'GwC_Cy

Cuts at:      0      87     184    1554    2151 
   Size:         87      97    1370     597

  Fragments arranged by size: 

                1370     597      97      87
 
PspOMI G'GGCC_C

Cuts at:      0    1541    2151 
   Size:       1541     610
 
PstI C_TGCA'G

Cuts at:      0     830    1118    1280    1457    1562    1574    1742    2151 
   Size:        830     288     162     177     105      12     168     409

  Fragments arranged by size: 

                 830     409     288     177     168     162     105      12
            
 
PvuII CAG'CTG

Cuts at:      0     348     600     732     927     999    1083    1275    1395 
   Size:        348     252     132     195      72      84     192     120

Cuts at:   1395    1855    2151 
   Size:        460     296

  Fragments arranged by size: 

                 460     348     296     252     195     192     132     120
                  84      72
 
SacI G_AGCT'C

Cuts at:      0     332     458    1370    2151 
   Size:        332     126     912     781

  Fragments arranged by size: 

                 912     781     332     126
 
SacII CC_GC'GG

Cuts at:      0    1285    2151 
   Size:       1285     866
 
SapI GCTCTTCn'nnn_

Cuts at:      0     272    2151 
   Size:        272    1879
 
SbfI CC_TGCA'GG

Cuts at:      0    1574    2151 
   Size:       1574     577
 
ScaI AGT'ACT

Cuts at:      0     244    2151 
   Size:        244    1907
 
SexAI A'CCwGG_T

Cuts at:      0      40    2151 
   Size:         40    2111
 
SfcI C'TryA_G

Cuts at:      0     826    1114    1276    1453    1558    1570    1738    2151 
   Size:        826     288     162     177     105      12     168     413

  Fragments arranged by size: 

                 826     413     288     177     168     162     105      12
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1144    2151 
   Size:       1144    1007
 
SfoI GGC'GCC

Cuts at:      0     955    2042    2151 
   Size:        955    1087     109

  Fragments arranged by size: 

                1087     955     109
 
StyI C'CwwG_G

Cuts at:      0    1778    1832    2105    2151 
   Size:       1778      54     273      46

  Fragments arranged by size: 

                1778     273      54      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1716    2151 
   Size:       1716     435
 
TatI w'GTAC_w

Cuts at:      0     242    1679    2151 
   Size:        242    1437     472

  Fragments arranged by size: 

                1437     472     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     188    2151 
   Size:        188    1963
 
XmnI GAAnn'nnTTC

Cuts at:      0     422    2151 
   Size:        422    1729
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI   BssHII   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FseI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MfeI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI    PflMI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     StyI
    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BcgI    BciVI
     BclI    BfrBI    BmgBI     BmtI    BpuEI    BsaBI    BsiWI    BspEI
    BsrDI    BsrGI    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FspI    FspAI
     HpaI     MluI     MscI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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