Restriction map for pF1KB5947
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       Bsp1407I    BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        MaeII       MamI        MfeI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        SacI        
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Alw44I        648  GTGCAC               G^TGCAC
ApaLI         648  GTGCAC               G^TGCAC
AvaI          430  CCCGGG               C^[CT]CG[GA]G
AviII        1469  TGCGCA               TGC^GCA
BalI          347  TGGCCA               TGG^CCA
BamHI        1280  GGATCC               G^GATCC
BanI          426  GGCGCC               G^G[CT][GA]CC
BbeI          426  GGCGCC               GGCGC^C
BbvCI        1519  CCTCAGC              CCTCAGC(-5/-2)
BglII         234  AGATCT               A^GATCT
BseRI        1399  GAGGAG               GAGGAG(10/8)
BsgI          205  CTGCAC               GTGCAG(16/14)
BsiEI        1308  CGGCCG               CG[GA][CT]^CG
BsmAI         727  GAGAC                GTCTC(1/5)
BsmBI         727  GAGACG               CGTCTC(1/5)
BsmI         1057  GCATTC               GAATGC(1/-1)
BsoBI         430  CCCGGG               C^[CT]CG[GA]G
BspDI          49  ATCGAT               AT^CGAT
BspMI        1466  ACCTGC               ACCTGC(4/8)
BstXI        1103  CCAACATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI           49  ATCGAT               AT^CGAT
CpoI          497  CGGTCCG              CG^G[AT]CCG
DraII         134  GGGACCC              [GA]G^G[ACGT]CC[CT]
EagI         1308  CGGCCG               C^GGCCG
EclXI        1308  CGGCCG               C^GGCCG
Eco52I       1308  CGGCCG               C^GGCCG
EcoO109I      134  GGGACCC              [GA]G^G[ACGT]CC[CT]
FspI         1469  TGCGCA               TGC^GCA
HincII       1005  GTTGAC               GT[CT]^[GA]AC
HindII       1005  GTTGAC               GT[CT]^[GA]AC
KasI          426  GGCGCC               G^GCGCC
KspI          476  CCGCGG               CCGC^GG
MluNI         347  TGGCCA               TGG^CCA
MscI          347  TGGCCA               TGG^CCA
NaeI          168  GCCGGC               GCC^GGC
NarI          426  GGCGCC               GG^CGCC
NgoMIV        168  GCCGGC               G^CCGGC
NspI          836  GCATGT               [GA]CATG^[CT]
PleI          966  GAGTC                GAGTC(4/5)
PpuMI         134  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1387  GACAACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           12  CTGCAG               CTGCA^G
PvuII         880  CAGCTG               CAG^CTG
RsrII         497  CGGTCCG              CG^G[AT]CCG
SacII         476  CCGCGG               CCGC^GG
SexAI         191  ACCTGGT              A^CC[AT]GGT
SfcI           12  CTGCAG               C^T[GA][CT]AG
SfoI          426  GGCGCC               GGC^GCC
SmaI          430  CCCGGG               CCC^GGG
SmlI          982  CTCAAG               C^T[CT][GA]AG
XcmI           77  CCATAGTCCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         430  CCCGGG               C^CCGGG
XmaI          430  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         408  CGCG                 CG^CG
              477  CGCG                
AcyI          426  GGCGCC               G[GA]^CG[CT]C
              847  GACGCC              
AspHI         471  GAGCAC               G[AT]GC[AT]^C
              648  GTGCAC              
BfaI          970  CTAG                 C^TAG
             1153  CTAG                
BglI          317  GCCAGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              850  GCCTTCCTGGC         
BsaHI         426  GGCGCC               G[GA]^CG[CT]C
              847  GACGCC              
BsiHKAI       471  GAGCAC               G[AT]GC[AT]^C
              648  GTGCAC              
BspHI         872  TCATGA               T^CATGA
             1415  TCATGA              
BsrFI         168  GCCGGC               [GA]^CCGG[CT]
             1448  ACCGGC              
BstEII        144  GGTTACC              G^GT[ACGT]ACC
              918  GGTCACC             
BstPI         144  GGTTACC              G^GT[ACGT]ACC
              918  GGTCACC             
BstUI         408  CGCG                 CG^CG
              477  CGCG                
Cfr10I        168  GCCGGC               [GA]^CCGG[CT]
             1448  ACCGGC              
EarI          816  GAAGAG               CTCTTC(1/4)
             1066  GAAGAG              
EcoO65I       144  GGTTACC              G^GT[ACGT]ACC
              918  GGTCACC             
FauI          132  GCGGG                CCCGC(4/6)
              478  GCGGG               
Hin1I         426  GGCGCC               G[GA]^CG[CT]C
              847  GACGCC              
Ksp632I       816  GAAGAG               CTCTTC(1/4)
             1066  GAAGAG              
MaeI          970  CTAG                 C^TAG
             1153  CTAG                
MseI         1211  TTAA                 T^TAA
             1235  TTAA                
MvnI          408  CGCG                 CG^CG
              477  CGCG                
RcaI          872  TCATGA               T^CATGA
             1415  TCATGA              
SapI          816  GAAGAGC              GCTCTTC(1/4)
             1066  GAAGAGC             
TfiI          587  GAATC                G^A[AT]TC
             1347  GATTC               
Tru9I        1211  TTAA                 T^TAA
             1235  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoNI       
EcoRII      Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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