Restriction map for pF1KB5947
 (Linear) (Six-base) MAPSORT of: KIBB5947.seq  Check: 1295  from: 1  to: 1527



KIBB5947.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     754     844     977    1527 
   Size:        620     134      90     133     550

  Fragments arranged by size: 

                 620     550     134     133      90
 
AleI CACnn'nnGTG

Cuts at:      0     805    1527 
   Size:        805     722
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     423     897    1356    1527 
   Size:        306     117     474     459     171

  Fragments arranged by size: 

                 474     459     306     171     117
 
ApaLI G'TGCA_C

Cuts at:      0     648    1527 
   Size:        648     879
 
AvaI C'yCGr_G

Cuts at:      0     430    1527 
   Size:        430    1097
 
BamHI G'GATC_C

Cuts at:      0    1280    1527 
   Size:       1280     247
 
BanI G'GyrC_C

Cuts at:      0     426    1527 
   Size:        426    1101
 
BanII G_rGCy'C

Cuts at:      0     221     371     932    1527 
   Size:        221     150     561     595

  Fragments arranged by size: 

                 595     561     221     150
 
BbeI G_GCGC'C

Cuts at:      0     430    1527 
   Size:        430    1097
 
BbvCI CC'TCA_GC

Cuts at:      0    1520    1527 
   Size:       1520       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     323     856    1527 
   Size:        323     533     671

  Fragments arranged by size: 

                 671     533     323
 
BglII A'GATC_T

Cuts at:      0     234    1527 
   Size:        234    1293
 
Bme1580I G_kGCm'C

Cuts at:      0     103     652     800    1527 
   Size:        103     549     148     727

  Fragments arranged by size: 

                 727     549     148     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     385    1378    1501    1527 
   Size:        292      93     993     123      26

  Fragments arranged by size: 

                 993     292     123      93      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     394    1126    1520    1527 
   Size:        394     732     394       7

  Fragments arranged by size: 

                 732     394     394       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1527 
   Size:        967     560
 
BsaHI Gr'CG_yC

Cuts at:      0     427     848    1527 
   Size:        427     421     679

  Fragments arranged by size: 

                 679     427     421
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     142     172     515     545     694     724    1527 
   Size:        142      30     343      30     149      30     803

  Fragments arranged by size: 

                 803     343     149     142      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1414    1527 
   Size:       1414     113
 
BseYI C'CCAG_C

Cuts at:      0     750    1527 
   Size:        750     777
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1527 
   Size:        190    1337
 
BsiEI CG_ry'CG

Cuts at:      0    1311    1527 
   Size:       1311     216
 
BsiHKAI G_wGCw'C

Cuts at:      0     475     652    1527 
   Size:        475     177     875

  Fragments arranged by size: 

                 875     475     177
 
BsmI GAATG_Cn'

Cuts at:      0    1057    1527 
   Size:       1057     470
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     721    1527 
   Size:        721     806
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     221     371     475     652     800     932    1527 
   Size:        103     118     150     104     177     148     132     595

  Fragments arranged by size: 

                 595     177     150     148     132     118     104     103
            
 
BspHI T'CATG_A

Cuts at:      0     872    1415    1527 
   Size:        872     543     112

  Fragments arranged by size: 

                 872     543     112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1475    1527 
   Size:       1475      52
 
BsrBI CCG'CTC

Cuts at:      0     281     414     747    1071    1527 
   Size:        281     133     333     324     456

  Fragments arranged by size: 

                 456     333     324     281     133
 
BsrFI r'CCGG_y

Cuts at:      0     168    1448    1527 
   Size:        168    1280      79

  Fragments arranged by size: 

                1280     168      79
 
BstEII G'GTnAC_C

Cuts at:      0     144     918    1527 
   Size:        144     774     609

  Fragments arranged by size: 

                 774     609     144
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1110    1527 
   Size:       1110     417
 
BstYI r'GATC_y

Cuts at:      0     234     545    1280    1527 
   Size:        234     311     735     247

  Fragments arranged by size: 

                 735     311     247     234
 
BtgI C'CryG_G

Cuts at:      0     311     476     694    1305    1527 
   Size:        311     165     218     611     222

  Fragments arranged by size: 

                 611     311     222     218     165
 
BtsI GCAGTG_nn'

Cuts at:      0     178    1479    1527 
   Size:        178    1301      48

  Fragments arranged by size: 

                1301     178      48
 
ClaI AT'CG_AT

Cuts at:      0      50    1527 
   Size:         50    1477
 
EaeI y'GGCC_r

Cuts at:      0     347     857    1308    1496    1527 
   Size:        347     510     451     188      31

  Fragments arranged by size: 

                 510     451     347     188      31
 
EagI C'GGCC_G

Cuts at:      0    1308    1527 
   Size:       1308     219
 
EarI CTCTTCn'nnn_

Cuts at:      0     811    1061    1527 
   Size:        811     250     466

  Fragments arranged by size: 

                 811     466     250
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     155     607    1527 
   Size:        155     452     920

  Fragments arranged by size: 

                 920     452     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     385     620     754     844     977    1378    1501 
   Size:        292      93     235     134      90     133     401     123

Cuts at:   1501    1527 
   Size:         26

  Fragments arranged by size: 

                 401     292     235     134     133     123      93      90
                  26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     826     859    1165    1322    1527 
   Size:        826      33     306     157     205

  Fragments arranged by size: 

                 826     306     205     157      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135    1527 
   Size:        135    1392
 
FspI TGC'GCA

Cuts at:      0    1471    1527 
   Size:       1471      56
 
HaeII r_GCGC'y

Cuts at:      0     430    1225    1440    1527 
   Size:        430     795     215      87

  Fragments arranged by size: 

                 795     430     215      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     537     569    1255    1287    1527 
   Size:        537      32     686      32     240

  Fragments arranged by size: 

                 686     537     240      32      32
 
HincII GTy'rAC

Cuts at:      0    1007    1527 
   Size:       1007     520
 
KasI G'GCGC_C

Cuts at:      0     426    1527 
   Size:        426    1101
 
MscI TGG'CCA

Cuts at:      0     349    1527 
   Size:        349    1178
 
MslI CAynn'nnrTG

Cuts at:      0      52     212     805    1111    1527 
   Size:         52     160     593     306     416

  Fragments arranged by size: 

                 593     416     306     160      52
 
MspA1I CmG'CkG

Cuts at:      0     478     882     894    1527 
   Size:        478     404      12     633

  Fragments arranged by size: 

                 633     478     404      12
 
NaeI GCC'GGC

Cuts at:      0     170    1527 
   Size:        170    1357
 
NarI GG'CG_CC

Cuts at:      0     427    1527 
   Size:        427    1100
 
NgoMIV G'CCGG_C

Cuts at:      0     168    1527 
   Size:        168    1359
 
NspI r_CATG'y

Cuts at:      0     840    1527 
   Size:        840     687
 
PasI CC'CwG_GG

Cuts at:      0     722    1324    1527 
   Size:        722     602     203

  Fragments arranged by size: 

                 722     602     203
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      91     897    1214    1527 
   Size:         91     806     317     313

  Fragments arranged by size: 

                 806     317     313      91
 
PfoI T'CCnGG_A

Cuts at:      0     269     458     500    1022    1527 
   Size:        269     189      42     522     505

  Fragments arranged by size: 

                 522     505     269     189      42
 
PpuMI rG'GwC_Cy

Cuts at:      0     135    1527 
   Size:        135    1392
 
PshAI GACnn'nnGTC

Cuts at:      0    1391    1527 
   Size:       1391     136
 
PstI C_TGCA'G

Cuts at:      0      16    1527 
   Size:         16    1511
 
PvuII CAG'CTG

Cuts at:      0     882    1527 
   Size:        882     645
 
RsrII CG'GwC_CG

Cuts at:      0     498    1527 
   Size:        498    1029
 
SacII CC_GC'GG

Cuts at:      0     479    1527 
   Size:        479    1048
 
SanDI GG'GwC_CC

Cuts at:      0     135    1527 
   Size:        135    1392
 
SapI GCTCTTCn'nnn_

Cuts at:      0     811    1061    1527 
   Size:        811     250     466

  Fragments arranged by size: 

                 811     466     250
 
SexAI A'CCwGG_T

Cuts at:      0     191    1527 
   Size:        191    1336
 
SfcI C'TryA_G

Cuts at:      0      12    1527 
   Size:         12    1515
 
SfoI GGC'GCC

Cuts at:      0     428    1527 
   Size:        428    1099
 
SmaI CCC'GGG

Cuts at:      0     432    1527 
   Size:        432    1095
 
SmlI C'TyrA_G

Cuts at:      0     982    1527 
   Size:        982     545
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     486     937    1318    1527 
   Size:        486     451     381     209

  Fragments arranged by size: 

                 486     451     381     209
 
TatI w'GTAC_w

Cuts at:      0    1178    1527 
   Size:       1178     349
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     482     514     515     547     694     726    1527 
   Size:        482      32       1      32     147      32     801

  Fragments arranged by size: 

                 801     482     147      32      32      32       1
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      84    1527 
   Size:         84    1443
 
XmaI C'CCGG_G

Cuts at:      0     430    1527 
   Size:        430    1097
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    ApaLI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BglI    BglII Bme1580I     BpmI   Bpu10I    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BstEII    BstXI
    BstYI     BtgI     BtsI     ClaI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII     KasI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII    RsrII    SacII
    SanDI     SapI    SexAI     SfcI     SfoI     SmaI     SmlI    TaqII
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaWI    BsiWI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI     SacI     SalI     SbfI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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