Restriction map for pF1KB5939
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
Cfr10I      CpoI        DraI        DraIII      Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MslI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        XbaI        XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI         1402  AAATTC               [GA]^AATT[CT]
AflIII        572  ACGTGT               A^C[GA][CT]GT
AhdI          487  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1309  GTGCAC               G^TGCAC
AlwNI        1072  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          399  GGGCCC               GGGCC^C
ApaLI        1309  GTGCAC               G^TGCAC
ApoI         1402  AAATTC               [GA]^AATT[CT]
AspEI         487  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1309  GTGCAC               G[AT]GC[AT]^C
AspI           70  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         399  GGGCCC               G[GA]GC[CT]^C
BclI         1497  TGATCA               T^GATCA
BsaBI        1441  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         938  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1309  GTGCAC               G[AT]GC[AT]^C
BsmBI        1234  GAGACG               CGTCTC(1/5)
BsmFI        1091  GGGAC                GGGAC(10/14)
BspDI         535  ATCGAT               AT^CGAT
BsrBI        1413  GAGCGG               CCGCTC(-3/-3)
BssHII         45  GCGCGC               G^CGCGC
BstAPI        510  GCAGTTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        657  GGTCACC              G^GT[ACGT]ACC
BstPI         657  GGTCACC              G^GT[ACGT]ACC
BstYI         854  AGATCC               [GA]^GATC[CT]
ClaI          535  ATCGAT               AT^CGAT
DraII        1454  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1351  GACAGGACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          938  CGGCCG               C^GGCCG
Eam1105I      487  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          928  GAAGAG               CTCTTC(1/4)
EclXI         938  CGGCCG               C^GGCCG
Eco52I        938  CGGCCG               C^GGCCG
EcoO109I     1454  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       657  GGTCACC              G^GT[ACGT]ACC
FbaI         1497  TGATCA               T^GATCA
HaeII         108  GGCGCT               [GA]GCGC^[CT]
Ksp632I       928  GAAGAG               CTCTTC(1/4)
MamI         1441  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          854  AGATCC               [GA]^GATC[CT]
NcoI         1183  CCATGG               C^CATGG
PflFI          70  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1454  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          434  CTGCAG               CTGCA^G
SfaNI         899  GCATC                GCATC(5/9)
SfcI          434  CTGCAG               C^T[GA][CT]AG
TfiI          988  GAATC                G^A[AT]TC
Tsp509I      1403  AATT                 ^AATT
Tth111I        70  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         854  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI           52  GACGCC               G[GA]^CG[CT]C
              247  GACGCC              
BbsI         1132  GAAGAC               GAAGAC(2/6)
             1489  GAAGAC              
BcnI          402  CCCGG                CC^[GC]GG
              936  CCCGG               
BpuAI        1132  GAAGAC               GAAGAC(2/6)
             1489  GAAGAC              
BsaHI          52  GACGCC               G[GA]^CG[CT]C
              247  GACGCC              
BsmAI         323  GAGAC                GTCTC(1/5)
             1234  GAGAC               
BspMI         128  ACCTGC               ACCTGC(4/8)
              671  ACCTGC              
BstXI         455  CCAGCGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1286  CCAACGAGGTGG        
DdeI          182  CTGAG                C^T[ACGT]AG
              814  CTGAG               
EcoNI         918  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              957  CCTGGAGGAGG         
HgaI           52  GACGC                GACGC(5/10)
              247  GACGC               
Hin1I          52  GACGCC               G[GA]^CG[CT]C
              247  GACGCC              
MseI          617  TTAA                 T^TAA
             1397  TTAA                
NciI          402  CCCGG                CC^[GC]GG
              936  CCCGG               
PaeR7I        904  CTCGAG               C^TCGAG
             1450  CTCGAG              
PflMI          72  CCACGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1183  CCATGGTGTGG         
Tru9I         617  TTAA                 T^TAA
             1397  TTAA                
Van91I         72  CCACGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1183  CCATGGTGTGG         
XhoI          904  CTCGAG               C^TCGAG
             1450  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BalI        BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DpnII       DsaI        EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SmlI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com