Restriction map for pF1KB5939
 (Linear) (Six-base) MAPSORT of: KIBB5939.seq  Check: 500  from: 1  to: 1524



KIBB5939.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     297     406     940    1524 
   Size:        229      68     109     534     584

  Fragments arranged by size: 

                 584     534     229     109      68
 
AflIII A'CryG_T

Cuts at:      0     572    1524 
   Size:        572     952
 
AhdI GACnn_n'nnGTC

Cuts at:      0     492    1524 
   Size:        492    1032
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1077    1524 
   Size:       1077     447
 
ApaI G_GGCC'C

Cuts at:      0     403    1524 
   Size:        403    1121
 
ApaLI G'TGCA_C

Cuts at:      0    1309    1524 
   Size:       1309     215
 
ApoI r'AATT_y

Cuts at:      0    1402    1524 
   Size:       1402     122
 
AvaI C'yCGr_G

Cuts at:      0      58     904    1450    1524 
   Size:         58     846     546      74

  Fragments arranged by size: 

                 846     546      74      58
 
BanII G_rGCy'C

Cuts at:      0     403    1524 
   Size:        403    1121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1139    1496    1524 
   Size:       1139     357      28

  Fragments arranged by size: 

                1139     357      28
 
BclI T'GATC_A

Cuts at:      0    1497    1524 
   Size:       1497      27
 
Bme1580I G_kGCm'C

Cuts at:      0     403     937    1313    1524 
   Size:        403     534     376     211

  Fragments arranged by size: 

                 534     403     376     211
 
BmgBI CAC'GTC

Cuts at:      0      75     489     573    1137    1524 
   Size:         75     414      84     564     387

  Fragments arranged by size: 

                 564     414     387      84      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1098    1237    1524 
   Size:       1098     139     287

  Fragments arranged by size: 

                1098     287     139
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     516    1524 
   Size:        516    1008
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1060    1429    1524 
   Size:        979      81     369      95

  Fragments arranged by size: 

                 979     369      95      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1312    1524 
   Size:       1312     212
 
BsaBI GATnn'nnATC

Cuts at:      0    1445    1524 
   Size:       1445      79
 
BsaHI Gr'CG_yC

Cuts at:      0      53     248    1524 
   Size:         53     195    1276

  Fragments arranged by size: 

                1276     195      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     562     592    1524 
   Size:        562      30     932

  Fragments arranged by size: 

                 932     562      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76      79     271     976     979    1009    1486    1524 
   Size:         76       3     192     705       3      30     477      38

  Fragments arranged by size: 

                 705     477     192      76      38      30       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     199     272    1524 
   Size:        199      73    1252

  Fragments arranged by size: 

                1252     199      73
 
BsiEI CG_ry'CG

Cuts at:      0     941    1524 
   Size:        941     583
 
BsiHKAI G_wGCw'C

Cuts at:      0    1313    1524 
   Size:       1313     211
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1228    1524 
   Size:       1228     296
 
Bsp1286I G_dGCh'C

Cuts at:      0     403     937    1313    1524 
   Size:        403     534     376     211

  Fragments arranged by size: 

                 534     403     376     211
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137     680    1524 
   Size:        137     543     844

  Fragments arranged by size: 

                 844     543     137
 
BsrBI CCG'CTC

Cuts at:      0    1415    1524 
   Size:       1415     109
 
BssHII G'CGCG_C

Cuts at:      0      45    1524 
   Size:         45    1479
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     516    1524 
   Size:        516    1008
 
BstEII G'GTnAC_C

Cuts at:      0     657    1524 
   Size:        657     867
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     462    1293    1524 
   Size:        462     831     231

  Fragments arranged by size: 

                 831     462     231
 
BstYI r'GATC_y

Cuts at:      0     854    1524 
   Size:        854     670
 
BtgI C'CryG_G

Cuts at:      0      29     251    1183    1524 
   Size:         29     222     932     341

  Fragments arranged by size: 

                 932     341     222      29
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1248    1524 
   Size:       1248     276
 
BtsI GCAGTG_nn'

Cuts at:      0     162    1194    1524 
   Size:        162    1032     330

  Fragments arranged by size: 

                1032     330     162
 
ClaI AT'CG_AT

Cuts at:      0     536    1524 
   Size:        536     988
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1357    1524 
   Size:       1357     167
 
EaeI y'GGCC_r

Cuts at:      0      26      32     159     236     938    1419    1524 
   Size:         26       6     127      77     702     481     105

  Fragments arranged by size: 

                 702     481     127     105      77      26       6
 
EagI C'GGCC_G

Cuts at:      0     938    1524 
   Size:        938     586
 
EarI CTCTTCn'nnn_

Cuts at:      0     923    1524 
   Size:        923     601
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     297     406     940     979    1060    1429    1524 
   Size:        229      68     109     534      39      81     369      95

  Fragments arranged by size: 

                 534     369     229     109      95      81      68      39
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     922     961    1524 
   Size:        922      39     563

  Fragments arranged by size: 

                 922     563      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1455    1524 
   Size:       1455      69
 
HaeII r_GCGC'y

Cuts at:      0     112    1524 
   Size:        112    1412
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510     562     594     740     772    1524 
   Size:        478      32      52      32     146      32     752

  Fragments arranged by size: 

                 752     478     146      52      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     587    1524 
   Size:        476     111     937

  Fragments arranged by size: 

                 937     476     111
 
MscI TGG'CCA

Cuts at:      0     161     238    1421    1524 
   Size:        161      77    1183     103

  Fragments arranged by size: 

                1183     161     103      77
 
MspA1I CmG'CkG

Cuts at:      0     199     352     433     699    1524 
   Size:        199     153      81     266     825

  Fragments arranged by size: 

                 825     266     199     153      81
 
NcoI C'CATG_G

Cuts at:      0    1183    1524 
   Size:       1183     341
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78    1189    1524 
   Size:         78    1111     335

  Fragments arranged by size: 

                1111     335      78
 
PfoI T'CCnGG_A

Cuts at:      0    1037    1406    1524 
   Size:       1037     369     118

  Fragments arranged by size: 

                1037     369     118
 
PpuMI rG'GwC_Cy

Cuts at:      0    1455    1524 
   Size:       1455      69
 
PspOMI G'GGCC_C

Cuts at:      0     399    1524 
   Size:        399    1125
 
PspXI vC'TCGA_Gb

Cuts at:      0     904    1524 
   Size:        904     620
 
PstI C_TGCA'G

Cuts at:      0     438    1524 
   Size:        438    1086
 
PvuII CAG'CTG

Cuts at:      0     352     433     699    1524 
   Size:        352      81     266     825

  Fragments arranged by size: 

                 825     352     266      81
 
SfcI C'TryA_G

Cuts at:      0     434    1524 
   Size:        434    1090
 
SmlI C'TyrA_G

Cuts at:      0     904    1327    1450    1524 
   Size:        904     423     123      74

  Fragments arranged by size: 

                 904     423     123      74
 
StyI C'CwwG_G

Cuts at:      0     151     239     338     443     662    1183    1524 
   Size:        151      88      99     105     219     521     341

  Fragments arranged by size: 

                 521     341     219     151     105      99      88
 
TatI w'GTAC_w

Cuts at:      0     977    1524 
   Size:        977     547
 
Tth111I GACn'n_nGTC

Cuts at:      0      73    1524 
   Size:         73    1451
 
XhoI C'TCGA_G

Cuts at:      0     904    1450    1524 
   Size:        904     546      74

  Fragments arranged by size: 

                 904     546      74
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    BanII     BbsI     BclI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI   BssHII   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     DrdI     EaeI     EagI
     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MscI
   MspA1I     NcoI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SfcI     SmlI     StyI     TatI  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI     BmtI   Bpu10I     BsaI    BsaAI    BsaWI     BsgI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI
    BstBI  BstZ17I   Bsu36I    CspCI     DraI   DraIII     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XbaI     XcmI     XmaI     XmnI     ZraI
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