Restriction map for pF1KB5787
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AvaI        
AviII       AvrII       BamHI       BbeI        BbvCI       
BcgI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BseAI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI         1381  GTCTAC               GT^[AC][GT]AC
AflIII       2462  ACATGT               A^C[GA][CT]GT
AhdI         2362  GACGCCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1849  GTGCAC               G^TGCAC
AlwI          951  GATCC                GGATC(4/5)
AocI          460  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1849  GTGCAC               G^TGCAC
AspEI        2362  GACGCCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2086  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1288  CACGTG               CAC^GTG
BciVI        1836  GGATAC               GTATCC(6/5)
BclI         1895  TGATCA               T^GATCA
BfaI         1456  CTAG                 C^TAG
BglII         281  AGATCT               A^GATCT
BsaAI        1288  CACGTG               [CT]AC^GT[GA]
BsaBI        1656  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1439  GAGACC               GGTCTC(1/5)
BsaWI         818  ACCGGA               [AT]^CCGG[AT]
BsgI         1036  GTGCAG               GTGCAG(16/14)
BsiEI        1503  CGACCG               CG[GA][CT]^CG
BsmBI          26  CGTCTC               CGTCTC(1/5)
BsmI         1017  GAATGC               GAATGC(1/-1)
BspLU11I     2462  ACATGT               A^CATGT
BsrDI        2192  GCAATG               GCAATG(2/0)
BssSI          11  CTCGTG               CACGAG(-5/-1)
Bsu36I        460  CCTGAGG              CC^T[ACGT]AGG
DrdI         1798  GACACAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2362  GACGCCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        460  CCTGAGG              CC^T[ACGT]AGG
FbaI         1895  TGATCA               T^GATCA
HaeII         147  GGCGCT               [GA]GCGC^[CT]
HincII       1783  GTCAAC               GT[CT]^[GA]AC
HindII       1783  GTCAAC               GT[CT]^[GA]AC
MaeI         1456  CTAG                 C^TAG
MamI         1656  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         1841  CATATG               CA^TATG
NheI         1455  GCTAGC               G^CTAGC
NspI         2462  ACATGT               [GA]CATG^[CT]
PflFI        2086  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1288  CACGTG               CAC^GTG
PmlI         1288  CACGTG               CAC^GTG
SexAI         687  ACCAGGT              A^CC[AT]GGT
SmlI          391  CTCAAG               C^T[CT][GA]AG
Tsp509I      2274  AATT                 ^AATT
Tth111I      2086  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcyI         1063  GACGCC               G[GA]^CG[CT]C
             2362  GACGCC              
BsaHI        1063  GACGCC               G[GA]^CG[CT]C
             2362  GACGCC              
BstYI         281  AGATCT               [GA]^GATC[CT]
              950  AGATCC              
DraIII        556  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1905  CACTGTGTG           
EarI         2107  GAAGAG               CTCTTC(1/4)
             2122  GAAGAG              
EcoNI          36  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1827  CCTGAAGCAGG         
Hin1I        1063  GACGCC               G[GA]^CG[CT]C
             2362  GACGCC              
Ksp632I      2107  GAAGAG               CTCTTC(1/4)
             2122  GAAGAG              
MflI          281  AGATCT               [GA]^GATC[CT]
              950  AGATCC              
PflMI         144  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1468  CCAGACAGTGG         
PleI         1756  GACTC                GAGTC(4/5)
              680  GAGTC               
PstI           55  CTGCAG               CTGCA^G
             1388  CTGCAG              
PvuII        1487  CAGCTG               CAG^CTG
             1885  CAGCTG              
SacI          544  GAGCTC               GAGCT^C
             2376  GAGCTC              
Van91I        144  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1468  CCAGACAGTGG         
XhoII         281  AGATCT               [GA]^GATC[CT]
              950  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XmnI        
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