Restriction map for pF1KB5913
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

Acc65I      AclI        AcsI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BseRI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         535  GACGTC               GACGT^C
AccI          448  GTCTAC               GT^[AC][GT]AC
AccII         810  CGCG                 CG^CG
AccIII       1322  TCCGGA               T^CCGGA
AcyI          535  GACGTC               G[GA]^CG[CT]C
AflII        1417  CTTAAG               C^TTAAG
AflIII        527  ACATGT               A^C[GA][CT]GT
AocI         1452  CCTCAGG              CC^T[ACGT]AGG
AspI          814  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         387  GGATCC               G^GATCC
BanI          670  GGCACC               G^G[CT][GA]CC
BciVI        1207  GTATCC               GTATCC(6/5)
BfrI         1417  CTTAAG               C^TTAAG
BsaHI         535  GACGTC               G[GA]^CG[CT]C
BsaI         1241  GAGACC               GGTCTC(1/5)
BseAI        1322  TCCGGA               T^CCGGA
BsiEI         474  CGACCG               CG[GA][CT]^CG
BsmAI        1241  GAGAC                GTCTC(1/5)
BsmFI         429  GTCCC                GGGAC(10/14)
BsmI         1068  GAATGC               GAATGC(1/-1)
BspEI        1322  TCCGGA               T^CCGGA
BspLU11I      527  ACATGT               A^CATGT
BsrBI        1189  GAGCGG               CCGCTC(-3/-3)
BstAPI        628  GCACTGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         810  CGCG                 CG^CG
Bsu36I       1452  CCTCAGG              CC^T[ACGT]AGG
DraIII       1138  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1452  CCTCAGG              CC^T[ACGT]AGG
EcoRV         546  GATATC               GAT^ATC
HgaI         1440  GCGTC                GACGC(5/10)
Hin1I         535  GACGTC               G[GA]^CG[CT]C
HincII       1354  GTCAAC               GT[CT]^[GA]AC
HindII       1354  GTCAAC               GT[CT]^[GA]AC
MaeIII        820  GTCAC                ^GT[ACGT]AC
MroI         1322  TCCGGA               T^CCGGA
MvnI          810  CGCG                 CG^CG
NcoI         1079  CCATGG               C^CATGG
NspI          527  ACATGT               [GA]CATG^[CT]
PflFI         814  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         1374  GAAGAGC              GCTCTTC(1/4)
SexAI        1139  ACCTGGT              A^CC[AT]GGT
SfaNI        1087  GATGC                GCATC(5/9)
SmlI         1417  CTTAAG               C^T[CT][GA]AG
StuI         1022  AGGCCT               AGG^CCT
Tsp45I        820  GTCAC                ^GT[GC]AC
Tsp509I       342  AATT                 ^AATT
Tth111I       814  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           96  CCAGACTCAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

ApaI          975  GGGCCC               GGGCC^C
             1280  GGGCCC              
AspHI         214  GAGCTC               G[AT]GC[AT]^C
             1178  GAGCTC              
AvaI         1073  CTCGGG               C^[CT]CG[GA]G
             1181  CTCGGG              
BalI           65  TGGCCA               TGG^CCA
             1445  TGGCCA              
BfaI         1096  CTAG                 C^TAG
             1198  CTAG                
BglI           67  GCCATTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              436  GCCAACCTGGC         
BlpI           76  GCTCAGC              GC^T[ACGT]AGC
             1186  GCTGAGC             
Bpu1102I       76  GCTCAGC              GC^T[ACGT]AGC
             1186  GCTGAGC             
BsgI          392  CTGCAC               GTGCAG(16/14)
             1026  CTGCAC              
BsiHKAI       214  GAGCTC               G[AT]GC[AT]^C
             1178  GAGCTC              
BsoBI        1073  CTCGGG               C^[CT]CG[GA]G
             1181  CTCGGG              
BsrDI         219  CATTGC               GCAATG(2/0)
              993  CATTGC              
BstXI         759  CCACATCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1163  CCAAGGGCCTGG        
BstYI         387  GGATCC               [GA]^GATC[CT]
              917  AGATCC              
CelII          76  GCTCAGC              GC^T[ACGT]AGC
             1186  GCTGAGC             
EarI          842  CTCTTC               CTCTTC(1/4)
             1374  GAAGAG              
EcoNI        1014  CCTAAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1413  CCTGCTTAAGG         
FauI          704  CCCGC                CCCGC(4/6)
             1191  GCGGG               
HaeII         359  AGCGCC               [GA]GCGC^[CT]
              676  AGCGCC              
Ksp632I       842  CTCTTC               CTCTTC(1/4)
             1374  GAAGAG              
MaeI         1096  CTAG                 C^TAG
             1198  CTAG                
MaeII         536  ACGT                 A^CGT
              556  ACGT                
MflI          387  GGATCC               [GA]^GATC[CT]
              917  AGATCC              
MluNI          65  TGGCCA               TGG^CCA
             1445  TGGCCA              
MscI           65  TGGCCA               TGG^CCA
             1445  TGGCCA              
MseI         1291  TTAA                 T^TAA
             1418  TTAA                
MspA1I         52  CAGCTG               C[AC]G^C[GT]G
              622  CAGCTG              
PpuMI         265  GGGTCCT              [GA]G^G[AT]CC[CT]
              427  GGGTCCC             
PvuII          52  CAGCTG               CAG^CTG
              622  CAGCTG              
SacI          214  GAGCTC               GAGCT^C
             1178  GAGCTC              
SbfI          966  CCTGCAGG             CCTGCA^GG
             1053  CCTGCAGG            
ScaI          284  AGTACT               AGT^ACT
             1046  AGTACT              
Sse8387I      966  CCTGCAGG             CCTGCA^GG
             1053  CCTGCAGG            
TfiI          789  GATTC                G^A[AT]TC
              941  GAATC               
Tru9I        1291  TTAA                 T^TAA
             1418  TTAA                
XhoII         387  GGATCC               [GA]^GATC[CT]
              917  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsaWI       BsiYI       BslI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TaqI        TseI        TspRI       TthHB8I     
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