################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspDI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I MamI MflI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1078 TCCGGA T^CCGGA AocI 454 CCTCAGG CC^T[ACGT]AGG AseI 964 ATTAAT AT^TAAT AsnI 964 ATTAAT AT^TAAT BanII 255 GAGCTC G[GA]GC[CT]^C BfaI 401 CTAG C^TAG BsaWI 1078 TCCGGA [AT]^CCGG[AT] BseAI 1078 TCCGGA T^CCGGA BsmI 1327 GAATGC GAATGC(1/-1) Bsp1407I 971 TGTACA T^GTACA BspEI 1078 TCCGGA T^CCGGA BspHI 822 TCATGA T^CATGA BsrDI 1139 GCAATG GCAATG(2/0) BsrGI 971 TGTACA T^GTACA BstF5I 1407 GGATG GGATG(2/0) Bsu36I 454 CCTCAGG CC^T[ACGT]AGG Eco81I 454 CCTCAGG CC^T[ACGT]AGG EcoRI 1072 GAATTC G^AATTC FauI 1463 GCGGG CCCGC(4/6) FokI 1407 GGATG GGATG(9/13) HincII 207 GTCAAC GT[CT]^[GA]AC HindII 207 GTCAAC GT[CT]^[GA]AC MaeI 401 CTAG C^TAG MaeII 819 ACGT A^CGT MboII 166 TCTTC GAAGA(8/7) MfeI 890 CAATTG C^AATTG MroI 1078 TCCGGA T^CCGGA MslI 858 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 890 CAATTG C^AATTG NcoI 1172 CCATGG C^CATGG PpuMI 1167 AGGACCC [GA]G^G[AT]CC[CT] PshBI 964 ATTAAT AT^TAAT RcaI 822 TCATGA T^CATGA SacI 255 GAGCTC GAGCT^C SexAI 1374 ACCAGGT A^CC[AT]GGT SfcI 387 CTACAG C^T[GA][CT]AG SmaI 61 CCCGGG CCC^GGG SspBI 971 TGTACA T^GTACA StuI 870 AGGCCT AGG^CCT TfiI 1534 GATTC G^A[AT]TC XmaCI 61 CCCGGG C^CCGGG XmaI 61 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 646 CGCG CG^CG 1459 CGCG AcsI 790 AAATTT [GA]^AATT[CT] 1072 GAATTC AfaI 480 GTAC GT^AC 972 GTAC AlwI 814 GGATC GGATC(4/5) 1557 GGATC ApoI 790 AAATTT [GA]^AATT[CT] 1072 GAATTC AspHI 255 GAGCTC G[AT]GC[AT]^C 1546 GAGCAC BglI 740 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1178 GCCGTGGAGGC BmyI 255 GAGCTC G[AGT]GC[ACT]^C 1546 GAGCAC BsaI 1014 GGTCTC GGTCTC(1/5) 1095 GGTCTC BsiHKAI 255 GAGCTC G[AT]GC[AT]^C 1546 GAGCAC BsmAI 1015 GTCTC GTCTC(1/5) 1096 GTCTC Bsp1286I 255 GAGCTC G[AGT]GC[ACT]^C 1546 GAGCAC BstUI 646 CGCG CG^CG 1459 CGCG EcoT14I 810 CCAAGG C^C[AT][AT]GG 1172 CCATGG KspI 645 CCGCGG CCGC^GG 1458 CCGCGG MvnI 646 CGCG CG^CG 1459 CGCG PleI 826 GACTC GAGTC(4/5) 895 GAGTC RsaI 480 GTAC GT^AC 972 GTAC SacII 645 CCGCGG CCGC^GG 1458 CCGCGG StyI 810 CCAAGG C^C[AT][AT]GG 1172 CCATGG TaqI 229 TCGA T^CGA 1440 TCGA Tsp45I 770 GTGAC ^GT[GC]AC 1271 GTGAC TthHB8I 229 TCGA T^CGA 1440 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AvaI AvaII BalI BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BsoBI BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI