Restriction map for pF1KB5906
 (Linear) (Six-base) MAPSORT of: KIBB5906.seq  Check: 2453  from: 1  to: 1578



KIBB5906.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125    1018    1019    1578 
   Size:        125     893       1     559

  Fragments arranged by size: 

                 893     559     125       1
 
ApoI r'AATT_y

Cuts at:      0     790    1072    1578 
   Size:        790     282     506

  Fragments arranged by size: 

                 790     506     282
 
AseI AT'TA_AT

Cuts at:      0     965    1578 
   Size:        965     613
 
AvaI C'yCGr_G

Cuts at:      0      61     327     803    1578 
   Size:         61     266     476     775

  Fragments arranged by size: 

                 775     476     266      61
 
BanII G_rGCy'C

Cuts at:      0     259    1578 
   Size:        259    1319
 
BglI GCCn_nnn'nGGC

Cuts at:      0     746    1184    1578 
   Size:        746     438     394

  Fragments arranged by size: 

                 746     438     394
 
BmgBI CAC'GTC

Cuts at:      0     820    1578 
   Size:        820     758
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1226    1578 
   Size:       1226     352
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1020    1101    1578 
   Size:       1020      81     477

  Fragments arranged by size: 

                1020     477      81
 
BsaWI w'CCGG_w

Cuts at:      0    1078    1578 
   Size:       1078     500
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1558    1578 
   Size:       1558      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1178    1235    1442    1578 
   Size:       1178      57     207     136

  Fragments arranged by size: 

                1178     207     136      57
 
BseYI C'CCAG_C

Cuts at:      0      56      77     316    1578 
   Size:         56      21     239    1262

  Fragments arranged by size: 

                1262     239      56      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     259    1550    1578 
   Size:        259    1291      28

  Fragments arranged by size: 

                1291     259      28
 
BsmI GAATG_Cn'

Cuts at:      0    1333    1578 
   Size:       1333     245
 
Bsp1286I G_dGCh'C

Cuts at:      0     259    1550    1578 
   Size:        259    1291      28

  Fragments arranged by size: 

                1291     259      28
 
BspEI T'CCGG_A

Cuts at:      0    1078    1578 
   Size:       1078     500
 
BspHI T'CATG_A

Cuts at:      0     822    1578 
   Size:        822     756
 
BsrDI GCAATG_nn'

Cuts at:      0    1146    1578 
   Size:       1146     432
 
BsrGI T'GTAC_A

Cuts at:      0     971    1578 
   Size:        971     607
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      40     223     601    1578 
   Size:         40     183     378     977

  Fragments arranged by size: 

                 977     378     183      40
 
Bsu36I CC'TnA_GG

Cuts at:      0     455    1578 
   Size:        455    1123
 
BtgI C'CryG_G

Cuts at:      0     131     636     645     741     774    1172    1179    1458 
   Size:        131     505       9      96      33     398       7     279

Cuts at:   1458    1470    1578 
   Size:         12     108

  Fragments arranged by size: 

                 505     398     279     131     108      96      33      12
                   9       7
 
BtsI GCAGTG_nn'

Cuts at:      0     498     573     666     711    1209    1578 
   Size:        498      75      93      45     498     369

  Fragments arranged by size: 

                 498     498     369      93      75      45
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1128    1163    1578 
   Size:       1128      35     415

  Fragments arranged by size: 

                1128     415      35
 
EaeI y'GGCC_r

Cuts at:      0     150     174     300     531     738    1215    1578 
   Size:        150      24     126     231     207     477     363

  Fragments arranged by size: 

                 477     363     231     207     150     126      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     779    1578 
   Size:        779     799
 
EcoICRI GAG'CTC

Cuts at:      0     257    1578 
   Size:        257    1321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125    1018    1019    1578 
   Size:        125     893       1     559

  Fragments arranged by size: 

                 893     559     125       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44    1168    1344    1578 
   Size:         44    1124     176     234

  Fragments arranged by size: 

                1124     234     176      44
 
EcoRI G'AATT_C

Cuts at:      0    1072    1578 
   Size:       1072     506
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     529     561     799     831    1578 
   Size:        529      32     238      32     747

  Fragments arranged by size: 

                 747     529     238      32      32
 
HincII GTy'rAC

Cuts at:      0     209    1578 
   Size:        209    1369
 
MfeI C'AATT_G

Cuts at:      0     890    1578 
   Size:        890     688
 
MscI TGG'CCA

Cuts at:      0     152     176     302     533    1578 
   Size:        152      24     126     231    1045

  Fragments arranged by size: 

                1045     231     152     126      24
 
MslI CAynn'nnrTG

Cuts at:      0     862    1578 
   Size:        862     716
 
MspA1I CmG'CkG

Cuts at:      0     614     647    1460    1578 
   Size:        614      33     813     118

  Fragments arranged by size: 

                 813     614     118      33
 
NcoI C'CATG_G

Cuts at:      0    1172    1578 
   Size:       1172     406
 
PasI CC'CwG_GG

Cuts at:      0     216    1578 
   Size:        216    1362
 
PpuMI rG'GwC_Cy

Cuts at:      0    1168    1578 
   Size:       1168     410
 
SacI G_AGCT'C

Cuts at:      0     259    1578 
   Size:        259    1319
 
SacII CC_GC'GG

Cuts at:      0     648    1461    1578 
   Size:        648     813     117

  Fragments arranged by size: 

                 813     648     117
 
SexAI A'CCwGG_T

Cuts at:      0    1374    1578 
   Size:       1374     204
 
SfcI C'TryA_G

Cuts at:      0     387    1578 
   Size:        387    1191
 
SmaI CCC'GGG

Cuts at:      0      63    1578 
   Size:         63    1515
 
SrfI GCCC'GGGC

Cuts at:      0      63    1578 
   Size:         63    1515
 
StuI AGG'CCT

Cuts at:      0     872    1578 
   Size:        872     706
 
StyI C'CwwG_G

Cuts at:      0     810    1172    1578 
   Size:        810     362     406

  Fragments arranged by size: 

                 810     406     362
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      70     865    1578 
   Size:         70     795     713

  Fragments arranged by size: 

                 795     713      70
 
TatI w'GTAC_w

Cuts at:      0     479     971    1578 
   Size:        479     492     607

  Fragments arranged by size: 

                 607     492     479
 
XmaI C'CCGG_G

Cuts at:      0      61    1578 
   Size:         61    1517
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     AvaI    BanII     BglI    BmgBI     BmrI
     BsaI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BsrDI    BsrGI    BstXI   Bsu36I     BtgI     BtsI
    CspCI     EaeI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I
   HincII     MfeI     MscI     MslI   MspA1I     NcoI     PasI    PpuMI
     SacI    SacII    SexAI     SfcI     SmaI     SrfI     StuI     StyI
    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
     BsgI    BsiEI    BsiWI    BsmBI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EarI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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