Restriction map for pF1KB5901
 (Linear) (Six-base) MAPSORT of: KIBB5901.seq  Check: 4191  from: 1  to: 1500



KIBB5901.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:      0    1012    1500 
   Size:       1012     488
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1063    1500 
   Size:       1063     437
 
AflII C'TTAA_G

Cuts at:      0     868    1500 
   Size:        868     632
 
AhdI GACnn_n'nnGTC

Cuts at:      0     739    1500 
   Size:        739     761
 
AleI CACnn'nnGTG

Cuts at:      0     645    1057    1500 
   Size:        645     412     443

  Fragments arranged by size: 

                 645     443     412
 
AlwNI CAG_nnn'CTG

Cuts at:      0     763    1500 
   Size:        763     737
 
ApaI G_GGCC'C

Cuts at:      0    1036    1500 
   Size:       1036     464
 
ApoI r'AATT_y

Cuts at:      0      61     700    1128    1140    1458    1500 
   Size:         61     639     428      12     318      42

  Fragments arranged by size: 

                 639     428     318      61      42      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1478    1500 
   Size:       1478      22
 
BanI G'GyrC_C

Cuts at:      0    1401    1500 
   Size:       1401      99
 
BanII G_rGCy'C

Cuts at:      0      19    1036    1500 
   Size:         19    1017     464

  Fragments arranged by size: 

                1017     464      19
 
BbvCI CC'TCA_GC

Cuts at:      0     469    1500 
   Size:        469    1031
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     867    1500 
   Size:        867     633
 
BfrBI ATG'CAT

Cuts at:      0     539    1500 
   Size:        539     961
 
Bme1580I G_kGCm'C

Cuts at:      0    1036    1406    1500 
   Size:       1036     370      94

  Fragments arranged by size: 

                1036     370      94
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1042    1500 
   Size:       1042     458
 
Bpu10I CC'TnA_GC

Cuts at:      0     469    1500 
   Size:        469    1031
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887    1500 
   Size:        887     613
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43    1500 
   Size:         43    1457
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1385    1500 
   Size:       1385     115
 
BsiHKAI G_wGCw'C

Cuts at:      0     757    1500 
   Size:        757     743
 
BsmI GAATG_Cn'

Cuts at:      0    1228    1500 
   Size:       1228     272
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     757    1036    1406    1500 
   Size:         19     738     279     370      94

  Fragments arranged by size: 

                 738     370     279      94      19
 
BsrBI CCG'CTC

Cuts at:      0      72    1500 
   Size:         72    1428
 
BsrDI GCAATG_nn'

Cuts at:      0     539    1112    1356    1500 
   Size:        539     573     244     144

  Fragments arranged by size: 

                 573     539     244     144
 
BsrGI T'GTAC_A

Cuts at:      0    1181    1500 
   Size:       1181     319
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     163    1500 
   Size:        163    1337
 
BstZ17I GTA'TAC

Cuts at:      0    1013    1500 
   Size:       1013     487
 
Bsu36I CC'TnA_GG

Cuts at:      0     546    1500 
   Size:        546     954
 
BtgI C'CryG_G

Cuts at:      0      23    1348    1500 
   Size:         23    1325     152

  Fragments arranged by size: 

                1325     152      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      35     281    1500 
   Size:         35     246    1219

  Fragments arranged by size: 

                1219     246      35
 
BtsI GCAGTG_nn'

Cuts at:      0     237     652    1368    1500 
   Size:        237     415     716     132

  Fragments arranged by size: 

                 716     415     237     132
 
DraI TTT'AAA

Cuts at:      0     119    1500 
   Size:        119    1381
 
EaeI y'GGCC_r

Cuts at:      0    1351    1500 
   Size:       1351     149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1063    1500 
   Size:       1063     437
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1033    1500 
   Size:       1033     467
 
EcoRI G'AATT_C

Cuts at:      0     700    1500 
   Size:        700     800
 
FspI TGC'GCA

Cuts at:      0      41    1500 
   Size:         41    1459
 
HaeII r_GCGC'y

Cuts at:      0     977    1500 
   Size:        977     523
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     670     702     725     757     823     855    1500 
   Size:        670      32      23      32      66      32     645

  Fragments arranged by size: 

                 670     645      66      32      32      32      23
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1222    1500 
   Size:       1222     278
 
MslI CAynn'nnrTG

Cuts at:      0     460     645     854    1057    1361    1500 
   Size:        460     185     209     203     304     139

  Fragments arranged by size: 

                 460     304     209     203     185     139
 
MspA1I CmG'CkG

Cuts at:      0      25    1350    1500 
   Size:         25    1325     150

  Fragments arranged by size: 

                1325     150      25
 
NdeI CA'TA_TG

Cuts at:      0     177    1500 
   Size:        177    1323
 
NsiI A_TGCA'T

Cuts at:      0     541    1500 
   Size:        541     959
 
NspI r_CATG'y

Cuts at:      0      46     613    1500 
   Size:         46     567     887

  Fragments arranged by size: 

                 887     567      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      55    1500 
   Size:         55    1445
 
PfoI T'CCnGG_A

Cuts at:      0     979    1500 
   Size:        979     521
 
PspOMI G'GGCC_C

Cuts at:      0    1032    1500 
   Size:       1032     468
 
SacII CC_GC'GG

Cuts at:      0      26    1351    1500 
   Size:         26    1325     149

  Fragments arranged by size: 

                1325     149      26
 
ScaI AGT'ACT

Cuts at:      0     143    1500 
   Size:        143    1357
 
SfcI C'TryA_G

Cuts at:      0    1481    1500 
   Size:       1481      19
 
SmlI C'TyrA_G

Cuts at:      0     868     902    1500 
   Size:        868      34     598

  Fragments arranged by size: 

                 868     598      34
 
SphI G_CATG'C

Cuts at:      0      46    1500 
   Size:         46    1454
 
TatI w'GTAC_w

Cuts at:      0     141    1181    1500 
   Size:        141    1040     319

  Fragments arranged by size: 

                1040     319     141
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      51      83     542     574     921     953    1500 
   Size:         51      32     459      32     347      32     547

  Fragments arranged by size: 

                 547     459     347      51      32      32      32
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AhdI     AleI    AlwNI     ApaI     ApoI
     BaeI     BanI    BanII    BbvCI    BciVI    BfrBI Bme1580I     BmrI
   Bpu10I    BpuEI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI
    BsrDI    BsrGI   BstAPI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     DraI     EaeI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
     MmeI     MslI   MspA1I     NdeI     NsiI     NspI    PflMI     PfoI
   PspOMI    SacII     ScaI     SfcI     SmlI     SphI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BbeI     BbsI     BcgI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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