Restriction map for pF1KB5782
 (Linear) (Six-base) MAPSORT of: KIBB5782.seq  Check: 1260  from: 1  to: 2307



KIBB5782.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1400    2307 
   Size:       1400     907
 
AatII G_ACGT'C

Cuts at:      0    1571    2307 
   Size:       1571     736
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     594     643    1915    2307 
   Size:        594      49    1272     392

  Fragments arranged by size: 

                1272     594     392      49
 
AfeI AGC'GCT

Cuts at:      0    1627    2307 
   Size:       1627     680
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2170    2307 
   Size:       2170     137
 
AlwNI CAG_nnn'CTG

Cuts at:      0      95     144    1373    1476    1551    1982    2307 
   Size:         95      49    1229     103      75     431     325

  Fragments arranged by size: 

                1229     431     325     103      95      75      49
 
ApaI G_GGCC'C

Cuts at:      0     122     468    1912    2307 
   Size:        122     346    1444     395

  Fragments arranged by size: 

                1444     395     346     122
 
ApaLI G'TGCA_C

Cuts at:      0      72    2307 
   Size:         72    2235
 
ApoI r'AATT_y

Cuts at:      0     892    2307 
   Size:        892    1415
 
AvaI C'yCGr_G

Cuts at:      0      49     697    1653    1695    2307 
   Size:         49     648     956      42     612

  Fragments arranged by size: 

                 956     648     612      49      42
 
BamHI G'GATC_C

Cuts at:      0     165    2307 
   Size:        165    2142
 
BanI G'GyrC_C

Cuts at:      0     134     826    1343    1784    2119    2307 
   Size:        134     692     517     441     335     188

  Fragments arranged by size: 

                 692     517     441     335     188     134
 
BanII G_rGCy'C

Cuts at:      0     122     468    1667    1711    1912    2307 
   Size:        122     346    1199      44     201     395

  Fragments arranged by size: 

                1199     395     346     201     122      44
 
BbeI G_GCGC'C

Cuts at:      0     830    2307 
   Size:        830    1477
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     272     988    1092    2307 
   Size:        272     716     104    1215

  Fragments arranged by size: 

                1215     716     272     104
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     483     517     551     585     876     910    2307 
   Size:        483      34      34      34     291      34    1397

  Fragments arranged by size: 

                1397     483     291      34      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0     107    2307 
   Size:        107    2200
 
BglI GCCn_nnn'nGGC

Cuts at:      0     497    1697    1814    2307 
   Size:        497    1200     117     493

  Fragments arranged by size: 

                1200     497     493     117
 
BlpI GC'TnA_GC

Cuts at:      0    1710    2307 
   Size:       1710     597
 
Bme1580I G_kGCm'C

Cuts at:      0      76     122     468    1846    1912    2122    2307 
   Size:         76      46     346    1378      66     210     185

  Fragments arranged by size: 

                1378     346     210     185      76      66      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1323    2229    2307 
   Size:       1323     906      78

  Fragments arranged by size: 

                1323     906      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2013    2307 
   Size:       2013     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     883    1029    1077    2047    2307 
   Size:        883     146      48     970     260

  Fragments arranged by size: 

                 970     883     260     146      48
 
Bpu10I CC'TnA_GC

Cuts at:      0    1518    2185    2307 
   Size:       1518     667     122

  Fragments arranged by size: 

                1518     667     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2212    2307 
   Size:       2212      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1355    2307 
   Size:       1355     952
 
BsaAI yAC'GTr

Cuts at:      0     618    2307 
   Size:        618    1689
 
BsaHI Gr'CG_yC

Cuts at:      0     713     827    1568    2307 
   Size:        713     114     741     739

  Fragments arranged by size: 

                 741     739     713     114
 
BsaWI w'CCGG_w

Cuts at:      0     679    2307 
   Size:        679    1628
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     574     604    1258    1288    1489    1519    2307 
   Size:        574      30     654      30     201      30     788

  Fragments arranged by size: 

                 788     654     574     201      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     897    1080    2307 
   Size:        897     183    1227

  Fragments arranged by size: 

                1227     897     183
 
BseYI C'CCAG_C

Cuts at:      0       3     822    1290    1524    2009    2307 
   Size:          3     819     468     234     485     298

  Fragments arranged by size: 

                 819     485     468     298     234       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111    1228    1336    2307 
   Size:        111    1117     108     971

  Fragments arranged by size: 

                1117     971     111     108
 
BsiEI CG_ry'CG

Cuts at:      0     492     852    2307 
   Size:        492     360    1455

  Fragments arranged by size: 

                1455     492     360
 
BsiHKAI G_wGCw'C

Cuts at:      0      76    2307 
   Size:         76    2231
 
BsmI GAATG_Cn'

Cuts at:      0     109    1077    2147    2307 
   Size:        109     968    1070     160

  Fragments arranged by size: 

                1070     968     160     109
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     122     468    1667    1711    1846    1912    2122 
   Size:         76      46     346    1199      44     135      66     210

Cuts at:   2122    2307 
   Size:        185

  Fragments arranged by size: 

                1199     346     210     185     135      76      66      46
                  44
 
BspEI T'CCGG_A

Cuts at:      0     679    2307 
   Size:        679    1628
 
BspHI T'CATG_A

Cuts at:      0     206    2282    2307 
   Size:        206    2076      25

  Fragments arranged by size: 

                2076     206      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     326     464     714    1308    1400    1990    2307 
   Size:        326     138     250     594      92     590     317

  Fragments arranged by size: 

                 594     590     326     317     250     138      92
 
BsrDI GCAATG_nn'

Cuts at:      0    1056    1167    2307 
   Size:       1056     111    1140

  Fragments arranged by size: 

                1140    1056     111
 
BsrFI r'CCGG_y

Cuts at:      0    1340    1781    2307 
   Size:       1340     441     526

  Fragments arranged by size: 

                1340     526     441
 
BsrGI T'GTAC_A

Cuts at:      0     875    2307 
   Size:        875    1432
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     134    1373    1799    1982    2307 
   Size:        134    1239     426     183     325

  Fragments arranged by size: 

                1239     426     325     183     134
 
BstBI TT'CG_AA

Cuts at:      0    1901    2307 
   Size:       1901     406
 
BstEII G'GTnAC_C

Cuts at:      0     963    2307 
   Size:        963    1344
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     975    1053    2307 
   Size:        975      78    1254

  Fragments arranged by size: 

                1254     975      78
 
BstYI r'GATC_y

Cuts at:      0     165    2307 
   Size:        165    2142
 
Bsu36I CC'TnA_GG

Cuts at:      0     427    2307 
   Size:        427    1880
 
BtgI C'CryG_G

Cuts at:      0    1022    1377    1753    2123    2307 
   Size:       1022     355     376     370     184

  Fragments arranged by size: 

                1022     376     370     355     184
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2085    2307 
   Size:       2085     222
 
BtsI GCAGTG_nn'

Cuts at:      0      19    1552    2307 
   Size:         19    1533     755

  Fragments arranged by size: 

                1533     755      19
 
DraIII CAC_nnn'GTG

Cuts at:      0     134    1256    1864    2307 
   Size:        134    1122     608     443

  Fragments arranged by size: 

                1122     608     443     134
 
EaeI y'GGCC_r

Cuts at:      0     193     489     849    1247    1635    1779    2307 
   Size:        193     296     360     398     388     144     528

  Fragments arranged by size: 

                 528     398     388     360     296     193     144
 
EagI C'GGCC_G

Cuts at:      0     489     849    2307 
   Size:        489     360    1458

  Fragments arranged by size: 

                1458     489     360
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     594     643     883    1029    1077    1915    2047    2307 
   Size:        594      49     240     146      48     838     132     260

  Fragments arranged by size: 

                 838     594     260     240     146     132      49      48
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      64    2307 
   Size:         64    2243
 
EcoO109I rG'GnC_Cy

Cuts at:      0     118     465     506    1320    1908    1909    1979    2092 
   Size:        118     347      41     814     588       1      70     113

Cuts at:   2092    2307 
   Size:        215

  Fragments arranged by size: 

                 814     588     347     215     118     113      70      41
                   1
 
EcoRI G'AATT_C

Cuts at:      0     892    2307 
   Size:        892    1415
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     165     328    1407    1929    2307 
   Size:        165     163    1079     522     378

  Fragments arranged by size: 

                1079     522     378     165     163
 
HaeII r_GCGC'y

Cuts at:      0     830    1291    1629    2211    2270    2307 
   Size:        830     461     338     582      59      37

  Fragments arranged by size: 

                 830     582     461     338      59      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     889     921    1489    1521    2307 
   Size:        157      32     700      32     568      32     786

  Fragments arranged by size: 

                 786     700     568     157      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     826    2307 
   Size:        826    1481
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    2307 
   Size:        665    1642
 
MslI CAynn'nnrTG

Cuts at:      0    2062    2307 
   Size:       2062     245
 
MspA1I CmG'CkG

Cuts at:      0      95     201     291     672     854     918    1688    1755 
   Size:         95     106      90     381     182      64     770      67

Cuts at:   1755    1790    1824    1953    2307 
   Size:         35      34     129     354

  Fragments arranged by size: 

                 770     381     354     182     129     106      95      90
                  67      64      35      34
 
NarI GG'CG_CC

Cuts at:      0     827    2307 
   Size:        827    1480
 
NcoI C'CATG_G

Cuts at:      0    1377    2307 
   Size:       1377     930
 
NsiI A_TGCA'T

Cuts at:      0     109    2307 
   Size:        109    2198
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1476    2307 
   Size:       1476     831
 
PfoI T'CCnGG_A

Cuts at:      0    1103    1262    2195    2307 
   Size:       1103     159     933     112

  Fragments arranged by size: 

                1103     933     159     112
 
PmlI CAC'GTG

Cuts at:      0     618    2307 
   Size:        618    1689
 
PpuMI rG'GwC_Cy

Cuts at:      0     506    1320    1979    2307 
   Size:        506     814     659     328

  Fragments arranged by size: 

                 814     659     506     328
 
PshAI GACnn'nnGTC

Cuts at:      0     658    2307 
   Size:        658    1649
 
PspOMI G'GGCC_C

Cuts at:      0     118     464    1908    2307 
   Size:        118     346    1444     399

  Fragments arranged by size: 

                1444     399     346     118
 
PstI C_TGCA'G

Cuts at:      0    1159    1411    1930    1952    2307 
   Size:       1159     252     519      22     355

  Fragments arranged by size: 

                1159     519     355     252      22
 
PvuII CAG'CTG

Cuts at:      0      95     201     291     672     918    1824    1953    2307 
   Size:         95     106      90     381     246     906     129     354

  Fragments arranged by size: 

                 906     381     354     246     129     106      95      90
            
 
SacII CC_GC'GG

Cuts at:      0    1756    2307 
   Size:       1756     551
 
ScaI AGT'ACT

Cuts at:      0    1597    2307 
   Size:       1597     710
 
SexAI A'CCwGG_T

Cuts at:      0     130    2307 
   Size:        130    2177
 
SfcI C'TryA_G

Cuts at:      0    1155    1407    1926    1948    2307 
   Size:       1155     252     519      22     359

  Fragments arranged by size: 

                1155     519     359     252      22
 
SfoI GGC'GCC

Cuts at:      0     828    2307 
   Size:        828    1479
 
SmaI CCC'GGG

Cuts at:      0    1655    1697    2307 
   Size:       1655      42     610

  Fragments arranged by size: 

                1655     610      42
 
SmlI C'TyrA_G

Cuts at:      0    2227    2307 
   Size:       2227      80
 
StyI C'CwwG_G

Cuts at:      0     365    1377    2307 
   Size:        365    1012     930

  Fragments arranged by size: 

                1012     930     365
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     670    2001    2307 
   Size:        670    1331     306

  Fragments arranged by size: 

                1331     670     306
 
TatI w'GTAC_w

Cuts at:      0     386     875    1595    1871    2201    2307 
   Size:        386     489     720     276     330     106

  Fragments arranged by size: 

                 720     489     386     330     276     106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     574     606    1425    1457    2307 
   Size:        574      32     819      32     850

  Fragments arranged by size: 

                 850     819     574      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1534    2307 
   Size:       1534     773
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1020    1101    2307 
   Size:       1020      81    1206

  Fragments arranged by size: 

                1206    1020      81
 
XmaI C'CCGG_G

Cuts at:      0    1653    1695    2307 
   Size:       1653      42     612

  Fragments arranged by size: 

                1653     612      42
 
ZraI GAC'GTC

Cuts at:      0    1569    2307 
   Size:       1569     738
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI
     MslI   MspA1I     NarI     NcoI     NsiI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII     ScaI    SexAI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI
     BclI    BglII    BmgBI     BmtI    BsaBI    BsiWI    BsmBI    BsrBI
   BssHII    BssSI  BstZ17I     ClaI    CspCI     DraI     DrdI     EarI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI     XmnI
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