Restriction map for pF1KB5869
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BanI        BbeI        BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
BstBI       BstEII      BstPI       BstUI       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          457  GTATAC               GT^[AC][GT]AC
AccIII       1227  TCCGGA               T^CCGGA
ApaI          231  GGGCCC               GGGCC^C
Asp700I         8  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          889  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          355  CCCGGG               C^[CT]CG[GA]G
BbrPI         519  CACGTG               CAC^GTG
BbsI          694  GAAGAC               GAAGAC(2/6)
BciVI        1224  GTATCC               GTATCC(6/5)
BfaI          718  CTAG                 C^TAG
BglI           59  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         694  GAAGAC               GAAGAC(2/6)
BsaAI         519  CACGTG               [CT]AC^GT[GA]
BsaI         1369  GAGACC               GGTCTC(1/5)
BseAI        1227  TCCGGA               T^CCGGA
BseRI         642  GAGGAG               GAGGAG(10/8)
BsoBI         355  CCCGGG               C^[CT]CG[GA]G
BspEI        1227  TCCGGA               T^CCGGA
BspLU11I      993  ACATGT               A^CATGT
BspMI        1241  GCAGGT               ACCTGC(4/8)
BsrBI         948  CCGCTC               CCGCTC(-3/-3)
Bst1107I      457  GTATAC               GTA^TAC
BstXI        1014  CCACAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       457  GTATAC               GTA^TAC
HaeII         255  AGCGCC               [GA]GCGC^[CT]
HgaI          555  GCGTC                GACGC(5/10)
HincII        307  GTCAAC               GT[CT]^[GA]AC
HindII        307  GTCAAC               GT[CT]^[GA]AC
MaeI          718  CTAG                 C^TAG
MaeII         520  ACGT                 A^CGT
MroI         1227  TCCGGA               T^CCGGA
NcoI          227  CCATGG               C^CATGG
NdeI          804  CATATG               CA^TATG
PflFI         889  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         519  CACGTG               CAC^GTG
PmlI          519  CACGTG               CAC^GTG
PshAI         550  GACAAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1378  CTGCAG               CTGCA^G
ScaI          575  AGTACT               AGT^ACT
SexAI         890  ACCAGGT              A^CC[AT]GGT
SfcI         1378  CTGCAG               C^T[GA][CT]AG
SmaI          355  CCCGGG               CCC^GGG
SmlI         1201  CTTGAG               C^T[CT][GA]AG
StuI         1205  AGGCCT               AGG^CCT
Tsp45I        761  GTGAC                ^GT[GC]AC
Tth111I       889  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          717  TCTAGA               T^CTAGA
XmaCI         355  CCCGGG               C^CCGGG
XmaI          355  CCCGGG               C^CCGGG
XmnI            8  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AfaI          576  GTAC                 GT^AC
             1123  GTAC                
AlwI          434  GATCC                GGATC(4/5)
              394  GGATC               
AocI          239  CCTCAGG              CC^T[ACGT]AGG
              437  CCTCAGG             
AspHI         245  GAGCAC               G[AT]GC[AT]^C
             1303  GAGCAC              
BalI           57  TGGCCA               TGG^CCA
              980  TGGCCA              
BpmI          260  CTCCAG               CTGGAG(16/14)
             1300  CTGGAG              
BsaWI        1001  ACCGGA               [AT]^CCGG[AT]
             1227  TCCGGA              
BsgI          170  GTGCAG               GTGCAG(16/14)
              961  GTGCAG              
BsiHKAI       245  GAGCAC               G[AT]GC[AT]^C
             1303  GAGCAC              
BsmFI         178  GGGAC                GGGAC(10/14)
             1331  GGGAC               
BspHI          23  TCATGA               T^CATGA
              690  TCATGA              
BstAPI        563  GCAACCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              670  GCAGAATGTGC         
BstYI         394  GGATCT               [GA]^GATC[CT]
              433  AGATCC              
Bsu36I        239  CCTCAGG              CC^T[ACGT]AGG
              437  CCTCAGG             
EaeI           57  TGGCCA               [CT]^GGCC[GA]
              980  TGGCCA              
EarI         1309  CTCTTC               CTCTTC(1/4)
              612  GAAGAG              
Eco81I        239  CCTCAGG              CC^T[ACGT]AGG
              437  CCTCAGG             
FauI           34  CCCGC                CCCGC(4/6)
             1374  CCCGC               
Ksp632I      1309  CTCTTC               CTCTTC(1/4)
              612  GAAGAG              
MaeIII        761  GTGAC                ^GT[ACGT]AC
              796  GTTAC               
MflI          394  GGATCT               [GA]^GATC[CT]
              433  AGATCC              
MluNI          57  TGGCCA               TGG^CCA
              980  TGGCCA              
MscI           57  TGGCCA               TGG^CCA
              980  TGGCCA              
MseI          404  TTAA                 T^TAA
             1130  TTAA                
MspA1I        567  CCGCTG               C[AC]G^C[GT]G
             1375  CCGCTG              
PpuMI         178  GGGACCC              [GA]G^G[AT]CC[CT]
              506  AGGACCT             
RcaI           23  TCATGA               T^CATGA
              690  TCATGA              
RsaI          576  GTAC                 GT^AC
             1123  GTAC                
TaqI          210  TCGA                 T^CGA
              781  TCGA                
Tru9I         404  TTAA                 T^TAA
             1130  TTAA                
Tsp509I        10  AATT                 ^AATT
              923  AATT                
TthHB8I       210  TCGA                 T^CGA
              781  TCGA                
XhoII         394  GGATCT               [GA]^GATC[CT]
              433  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AflIII      AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        TseI        TspRI       
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