Restriction map for pF1KB5869
 (Linear) (Six-base) MAPSORT of: KIBB5869.seq  Check: 2081  from: 1  to: 1389



KIBB5869.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:      0     458    1389 
   Size:        458     931
 
AflIII A'CryG_T

Cuts at:      0     518     520     993    1389 
   Size:        518       2     473     396

  Fragments arranged by size: 

                 518     473     396       2
 
AleI CACnn'nnGTG

Cuts at:      0     413    1389 
   Size:        413     976
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     364     396    1389 
   Size:        364      32     993

  Fragments arranged by size: 

                 993     364      32
 
ApaI G_GGCC'C

Cuts at:      0     235    1389 
   Size:        235    1154
 
AvaI C'yCGr_G

Cuts at:      0     355    1389 
   Size:        355    1034
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     131     164    1389 
   Size:        131      33    1225

  Fragments arranged by size: 

                1225     131      33
 
BanII G_rGCy'C

Cuts at:      0      85     202     235    1389 
   Size:         85     117      33    1154

  Fragments arranged by size: 

                1154     117      85      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     701    1389 
   Size:        701     688
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1235    1389 
   Size:       1235     154
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65    1389 
   Size:         65    1324
 
Bme1580I G_kGCm'C

Cuts at:      0     156     235    1014    1389 
   Size:        156      79     779     375

  Fragments arranged by size: 

                 779     375     156      79
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     139    1111    1389 
   Size:        139     972     278

  Fragments arranged by size: 

                 972     278     139
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1321    1389 
   Size:        245    1076      68

  Fragments arranged by size: 

                1076     245      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1222    1389 
   Size:       1222     167
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1363    1389 
   Size:       1363      26
 
BsaAI yAC'GTr

Cuts at:      0     521    1389 
   Size:        521     868
 
BsaWI w'CCGG_w

Cuts at:      0    1001    1227    1389 
   Size:       1001     226     162

  Fragments arranged by size: 

                1001     226     162
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     657    1389 
   Size:        657     732
 
BseYI C'CCAG_C

Cuts at:      0     127     286    1021    1269    1389 
   Size:        127     159     735     248     120

  Fragments arranged by size: 

                 735     248     159     127     120
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     982    1389 
   Size:        191     791     407

  Fragments arranged by size: 

                 791     407     191
 
BsiHKAI G_wGCw'C

Cuts at:      0     249    1307    1389 
   Size:        249    1058      82

  Fragments arranged by size: 

                1058     249      82
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     156     202     235     249    1014    1307    1389 
   Size:         85      71      46      33      14     765     293      82

  Fragments arranged by size: 

                 765     293      85      82      71      46      33      14
            
 
BspEI T'CCGG_A

Cuts at:      0    1227    1389 
   Size:       1227     162
 
BspHI T'CATG_A

Cuts at:      0      23     690    1389 
   Size:         23     667     699

  Fragments arranged by size: 

                 699     667      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1232    1389 
   Size:       1232     157
 
BsrBI CCG'CTC

Cuts at:      0     950    1389 
   Size:        950     439
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     569     676    1389 
   Size:        569     107     713

  Fragments arranged by size: 

                 713     569     107
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1021    1389 
   Size:       1021     368
 
BstYI r'GATC_y

Cuts at:      0     394     433    1389 
   Size:        394      39     956

  Fragments arranged by size: 

                 956     394      39
 
BstZ17I GTA'TAC

Cuts at:      0     459    1389 
   Size:        459     930
 
Bsu36I CC'TnA_GG

Cuts at:      0     240     438    1389 
   Size:        240     198     951

  Fragments arranged by size: 

                 951     240     198
 
BtgI C'CryG_G

Cuts at:      0     157     227     983    1389 
   Size:        157      70     756     406

  Fragments arranged by size: 

                 756     406     157      70
 
BtsI GCAGTG_nn'

Cuts at:      0     179    1014    1389 
   Size:        179     835     375

  Fragments arranged by size: 

                 835     375     179
 
EaeI y'GGCC_r

Cuts at:      0      57     980    1389 
   Size:         57     923     409

  Fragments arranged by size: 

                 923     409      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     607    1315    1389 
   Size:        607     708      74

  Fragments arranged by size: 

                 708     607      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1321    1389 
   Size:        245    1076      68

  Fragments arranged by size: 

                1076     245      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     179     507    1146    1389 
   Size:        179     328     639     243

  Fragments arranged by size: 

                 639     328     243     179
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     745    1389 
   Size:        745     644
 
HaeII r_GCGC'y

Cuts at:      0     259    1389 
   Size:        259    1130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453    1389 
   Size:        421      32     936

  Fragments arranged by size: 

                 936     421      32
 
HincII GTy'rAC

Cuts at:      0     309    1389 
   Size:        309    1080
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1076    1178    1346    1389 
   Size:       1076     102     168      43

  Fragments arranged by size: 

                1076     168     102      43
 
MscI TGG'CCA

Cuts at:      0      59     982    1389 
   Size:         59     923     407

  Fragments arranged by size: 

                 923     407      59
 
MslI CAynn'nnrTG

Cuts at:      0     413     607     703     769    1084    1360    1389 
   Size:        413     194      96      66     315     276      29

  Fragments arranged by size: 

                 413     315     276     194      96      66      29
 
MspA1I CmG'CkG

Cuts at:      0     569    1377    1389 
   Size:        569     808      12

  Fragments arranged by size: 

                 808     569      12
 
NcoI C'CATG_G

Cuts at:      0     227    1389 
   Size:        227    1162
 
NdeI CA'TA_TG

Cuts at:      0     805    1389 
   Size:        805     584
 
NspI r_CATG'y

Cuts at:      0     702     997    1083    1389 
   Size:        702     295      86     306

  Fragments arranged by size: 

                 702     306     295      86
 
PasI CC'CwG_GG

Cuts at:      0     195    1389 
   Size:        195    1194
 
PciI A'CATG_T

Cuts at:      0     993    1389 
   Size:        993     396
 
PfoI T'CCnGG_A

Cuts at:      0     261    1389 
   Size:        261    1128
 
PmlI CAC'GTG

Cuts at:      0     521    1389 
   Size:        521     868
 
PpuMI rG'GwC_Cy

Cuts at:      0     179     507    1389 
   Size:        179     328     882

  Fragments arranged by size: 

                 882     328     179
 
PshAI GACnn'nnGTC

Cuts at:      0     554    1389 
   Size:        554     835
 
PspOMI G'GGCC_C

Cuts at:      0     231    1389 
   Size:        231    1158
 
PstI C_TGCA'G

Cuts at:      0    1382    1389 
   Size:       1382       7
 
SanDI GG'GwC_CC

Cuts at:      0     179    1389 
   Size:        179    1210
 
ScaI AGT'ACT

Cuts at:      0     577    1389 
   Size:        577     812
 
SexAI A'CCwGG_T

Cuts at:      0     890    1389 
   Size:        890     499
 
SfcI C'TryA_G

Cuts at:      0    1378    1389 
   Size:       1378      11
 
SmaI CCC'GGG

Cuts at:      0     357    1389 
   Size:        357    1032
 
SmlI C'TyrA_G

Cuts at:      0    1201    1389 
   Size:       1201     188
 
StuI AGG'CCT

Cuts at:      0    1207    1389 
   Size:       1207     182
 
StyI C'CwwG_G

Cuts at:      0     227     881    1142    1389 
   Size:        227     654     261     247

  Fragments arranged by size: 

                 654     261     247     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     300    1389 
   Size:        300    1089
 
TatI w'GTAC_w

Cuts at:      0     575    1122    1389 
   Size:        575     547     267

  Fragments arranged by size: 

                 575     547     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     153     185    1389 
   Size:        153      32    1204

  Fragments arranged by size: 

                1204     153      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     892    1389 
   Size:        892     497
 
XbaI T'CTAG_A

Cuts at:      0     717    1389 
   Size:        717     672
 
XmaI C'CCGG_G

Cuts at:      0     355    1389 
   Size:        355    1034
 
XmnI GAAnn'nnTTC

Cuts at:      0      12    1389 
   Size:         12    1377
 

 Enzymes that do cut:

     AccI   AflIII     AleI     AloI     ApaI     AvaI     BaeI    BanII
     BbsI    BciVI     BglI Bme1580I     BmrI     BpmI    BpuEI     BsaI
    BsaAI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NspI     PasI     PciI     PfoI     PmlI    PpuMI    PshAI   PspOMI
     PstI    SanDI     ScaI    SexAI     SfcI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsaHI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XcmI     XhoI     ZraI
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