Restriction map for pF1KB5861
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflIII      Alw44I      ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AvrII       BalI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BspEI       BspLU11I    
BspMI       BsrDI       BssHII      Bst1107I    BstBI       
BstZ17I     CpoI        DraI        DraIII      DrdI        
DsaI        EagI        EclXI       Eco52I      EcoRI       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tsp509I     Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I        158  GGTACC               G^GTACC
AflII         544  CTTAAG               C^TTAAG
AgeI          155  ACCGGT               A^CCGGT
AhdI          123  GACTGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       530  AGCGCT               AGC^GCT
ApaI           13  GGGCCC               GGGCC^C
Asp718I       158  GGTACC               G^GTACC
AspEI         123  GACTGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          268  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         881  TGCGCA               TGC^GCA
BamHI         369  GGATCC               G^GATCC
BbeI           96  GGCGCC               GGCGC^C
BfaI         1123  CTAG                 C^TAG
BfrI          544  CTTAAG               C^TTAAG
BlpI          252  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      252  GCTGAGC              GC^T[ACGT]AGC
BsaI          112  GAGACC               GGTCTC(1/5)
BsaWI         155  ACCGGT               [AT]^CCGG[AT]
BsgI         1180  CTGCAC               GTGCAG(16/14)
BsmI         1083  GAATGC               GAATGC(1/-1)
Bsp1407I      378  TGTACA               T^GTACA
BspDI         175  ATCGAT               AT^CGAT
BspHI         516  TCATGA               T^CATGA
BsrGI         378  TGTACA               T^GTACA
BstAPI       1170  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1306  GGTGACC              G^GT[ACGT]ACC
BstPI        1306  GGTGACC              G^GT[ACGT]ACC
BstXI         809  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         369  GGATCC               [GA]^GATC[CT]
CelII         252  GCTGAGC              GC^T[ACGT]AGC
ClaI          175  ATCGAT               AT^CGAT
EaeI          479  CGGCCA               [CT]^GGCC[GA]
Eam1105I      123  GACTGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      530  AGCGCT               AGC^GCT
EcoO65I      1306  GGTGACC              G^GT[ACGT]ACC
EcoRV         757  GATATC               GAT^ATC
FspI          881  TGCGCA               TGC^GCA
HincII        655  GTCAAC               GT[CT]^[GA]AC
HindII        655  GTCAAC               GT[CT]^[GA]AC
KasI           96  GGCGCC               G^GCGCC
KpnI          158  GGTACC               GGTAC^C
MaeI         1123  CTAG                 C^TAG
MflI          369  GGATCC               [GA]^GATC[CT]
NaeI          632  GCCGGC               GCC^GGC
NarI           96  GGCGCC               GG^CGCC
NgoMIV        632  GCCGGC               G^CCGGC
PaeR7I       1345  CTCGAG               C^TCGAG
PflFI         268  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         155  ACCGGT               A^CCGGT
PpuMI         488  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          516  TCATGA               T^CATGA
SacI          304  GAGCTC               GAGCT^C
SfoI           96  GGCGCC               GGC^GCC
SphI          884  GCATGC               GCATG^C
SspBI         378  TGTACA               T^GTACA
StuI          141  AGGCCT               AGG^CCT
TfiI          396  GAATC                G^A[AT]TC
Tth111I       268  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          374  CCAATGTACAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1345  CTCGAG               C^TCGAG
XhoII         369  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI           96  GGCGCC               G[GA]^CG[CT]C
              358  GACGCC              
AlwNI         787  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1375  CAGCATCTG           
AocI         1246  CCTGAGG              CC^T[ACGT]AGG
             1357  CCTGAGG             
AspHI         304  GAGCTC               G[AT]GC[AT]^C
              643  GAGCAC              
BanII          13  GGGCCC               G[GA]GC[CT]^C
              304  GAGCTC              
BbvCI        1101  CCTCAGC              CCTCAGC(-5/-2)
             1038  GCTGAGG             
BglI           67  GCCGTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1066  GCCCGGGAGGC         
BsaHI          96  GGCGCC               G[GA]^CG[CT]C
              358  GACGCC              
BsiHKAI       304  GAGCTC               G[AT]GC[AT]^C
              643  GAGCAC              
BsmBI         187  GAGACG               CGTCTC(1/5)
              558  GAGACG              
BsrBI         243  GAGCGG               CCGCTC(-3/-3)
             1348  GAGCGG              
BssSI         553  CACGAG               CACGAG(-5/-1)
              931  CTCGTG              
Bsu36I       1246  CCTGAGG              CC^T[ACGT]AGG
             1357  CCTGAGG             
Eco81I       1246  CCTGAGG              CC^T[ACGT]AGG
             1357  CCTGAGG             
EcoNI         162  CCTCCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1101  CCTCAGCCAGG         
EcoT14I       809  CCAAGG               C^C[AT][AT]GG
             1088  CCAAGG              
HgaI          358  GACGC                GACGC(5/10)
              505  GACGC               
Hin1I          96  GGCGCC               G[GA]^CG[CT]C
              358  GACGCC              
HphI         1306  GGTGA                GGTGA(8/7)
              806  TCACC               
MaeII         284  ACGT                 A^CGT
              659  ACGT                
MseI          545  TTAA                 T^TAA
              728  TTAA                
MspA1I         40  CCGCTG               C[AC]G^C[GT]G
             1192  CAGCGG              
PleI          129  GAGTC                GAGTC(4/5)
              574  GAGTC               
PstI          214  CTGCAG               CTGCA^G
             1165  CTGCAG              
SapI          619  GAAGAGC              GCTCTTC(1/4)
             1033  GAAGAGC             
SfcI          214  CTGCAG               C^T[GA][CT]AG
             1165  CTGCAG              
SmaI          485  CCCGGG               CCC^GGG
             1067  CCCGGG              
StyI          809  CCAAGG               C^C[AT][AT]GG
             1088  CCAAGG              
Tru9I         545  TTAA                 T^TAA
              728  TTAA                
XmaCI         485  CCCGGG               C^CCGGG
             1067  CCCGGG              
XmaI          485  CCCGGG               C^CCGGG
             1067  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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