Restriction map for pF1KB5861
 (Linear) (Six-base) MAPSORT of: KIBB5861.seq  Check: 872  from: 1  to: 1383



KIBB5861.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

Acc65I G'GTAC_C

Cuts at:      0     158    1383 
   Size:        158    1225
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1313    1383 
   Size:       1313      70
 
AfeI AGC'GCT

Cuts at:      0     532    1383 
   Size:        532     851
 
AflII C'TTAA_G

Cuts at:      0     544    1383 
   Size:        544     839
 
AgeI A'CCGG_T

Cuts at:      0     155    1383 
   Size:        155    1228
 
AhdI GACnn_n'nnGTC

Cuts at:      0     128    1383 
   Size:        128    1255
 
AlwNI CAG_nnn'CTG

Cuts at:      0     792    1380    1383 
   Size:        792     588       3

  Fragments arranged by size: 

                 792     588       3
 
ApaI G_GGCC'C

Cuts at:      0      17    1383 
   Size:         17    1366
 
AvaI C'yCGr_G

Cuts at:      0      17     485    1067    1345    1383 
   Size:         17     468     582     278      38

  Fragments arranged by size: 

                 582     468     278      38      17
 
BamHI G'GATC_C

Cuts at:      0     369    1383 
   Size:        369    1014
 
BanI G'GyrC_C

Cuts at:      0      96     158     635    1368    1383 
   Size:         96      62     477     733      15

  Fragments arranged by size: 

                 733     477      96      62      15
 
BanII G_rGCy'C

Cuts at:      0      17     308    1383 
   Size:         17     291    1075

  Fragments arranged by size: 

                1075     291      17
 
BbeI G_GCGC'C

Cuts at:      0     100    1383 
   Size:        100    1283
 
BbvCI CC'TCA_GC

Cuts at:      0    1039    1102    1383 
   Size:       1039      63     281

  Fragments arranged by size: 

                1039     281      63
 
BglI GCCn_nnn'nGGC

Cuts at:      0      73    1072    1383 
   Size:         73     999     311

  Fragments arranged by size: 

                 999     311      73
 
BlpI GC'TnA_GC

Cuts at:      0     253    1383 
   Size:        253    1130
 
Bme1580I G_kGCm'C

Cuts at:      0      17    1373    1383 
   Size:         17    1356      10

  Fragments arranged by size: 

                1356      17      10
 
BmgBI CAC'GTC

Cuts at:      0     285    1383 
   Size:        285    1098
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     492     589     672    1383 
   Size:        492      97      83     711

  Fragments arranged by size: 

                 711     492      97      83
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      43    1383 
   Size:         43    1340
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     148     205     481     592     814     838     961 
   Size:        106      42      57     276     111     222      24     123

Cuts at:    961    1165    1283    1383 
   Size:        204     118     100

  Fragments arranged by size: 

                 276     222     204     123     118     111     106     100
                  57      42      24
 
Bpu10I CC'TnA_GC

Cuts at:      0    1039    1102    1383 
   Size:       1039      63     281

  Fragments arranged by size: 

                1039     281      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     328    1243    1383 
   Size:        292      36     915     140

  Fragments arranged by size: 

                 915     292     140      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     106    1383 
   Size:        106    1277
 
BsaHI Gr'CG_yC

Cuts at:      0      97     359    1383 
   Size:         97     262    1024

  Fragments arranged by size: 

                1024     262      97
 
BsaWI w'CCGG_w

Cuts at:      0     155    1383 
   Size:        155    1228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     811     841    1383 
   Size:        811      30     542

  Fragments arranged by size: 

                 811     542      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     601     712     859     883     886     958    1069 
   Size:        124     477     111     147      24       3      72     111

Cuts at:   1069    1383 
   Size:        314

  Fragments arranged by size: 

                 477     314     147     124     111     111      72      24
                   3
 
BseYI C'CCAG_C

Cuts at:      0     995    1094    1293    1373    1383 
   Size:        995      99     199      80      10

  Fragments arranged by size: 

                 995     199      99      80      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1383 
   Size:       1165     218
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     647    1383 
   Size:        308     339     736

  Fragments arranged by size: 

                 736     339     308
 
BsmI GAATG_Cn'

Cuts at:      0    1089    1383 
   Size:       1089     294
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     181     552    1383 
   Size:        181     371     831

  Fragments arranged by size: 

                 831     371     181
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     308     647    1373    1383 
   Size:         17     291     339     726      10

  Fragments arranged by size: 

                 726     339     291      17      10
 
BspHI T'CATG_A

Cuts at:      0     516    1383 
   Size:        516     867
 
BsrBI CCG'CTC

Cuts at:      0     245    1350    1383 
   Size:        245    1105      33

  Fragments arranged by size: 

                1105     245      33
 
BsrFI r'CCGG_y

Cuts at:      0     155     632    1303    1383 
   Size:        155     477     671      80

  Fragments arranged by size: 

                 671     477     155      80
 
BsrGI T'GTAC_A

Cuts at:      0     378    1383 
   Size:        378    1005
 
BssSI C'ACGA_G

Cuts at:      0     553     931    1383 
   Size:        553     378     452

  Fragments arranged by size: 

                 553     452     378
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1176    1383 
   Size:       1176     207
 
BstEII G'GTnAC_C

Cuts at:      0    1306    1383 
   Size:       1306      77
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     816    1383 
   Size:        816     567
 
BstYI r'GATC_y

Cuts at:      0     369    1383 
   Size:        369    1014
 
Bsu36I CC'TnA_GG

Cuts at:      0    1247    1358    1383 
   Size:       1247     111      25

  Fragments arranged by size: 

                1247     111      25
 
ClaI AT'CG_AT

Cuts at:      0     176    1383 
   Size:        176    1207
 
EaeI y'GGCC_r

Cuts at:      0     479    1383 
   Size:        479     904
 
EarI CTCTTCn'nnn_

Cuts at:      0     515     614     808    1028    1046    1150    1383 
   Size:        515      99     194     220      18     104     233

  Fragments arranged by size: 

                 515     233     220     194     104      99      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     619    1383 
   Size:        619     764
 
EcoICRI GAG'CTC

Cuts at:      0     306    1383 
   Size:        306    1077
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     148     205     481     592     814     838     961 
   Size:        106      42      57     276     111     222      24     123

Cuts at:    961    1165    1283    1313    1383 
   Size:        204     118      30      70

  Fragments arranged by size: 

                 276     222     204     123     118     111     106      70
                  57      42      30      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     166    1105    1383 
   Size:        166     939     278

  Fragments arranged by size: 

                 939     278     166
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     489    1230    1383 
   Size:         14     475     741     153

  Fragments arranged by size: 

                 741     475     153      14
 
EcoRV GAT'ATC

Cuts at:      0     759    1383 
   Size:        759     624
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     759    1383 
   Size:        759     624
 
FspI TGC'GCA

Cuts at:      0     883    1383 
   Size:        883     500
 
HaeII r_GCGC'y

Cuts at:      0     100     534     633    1047    1101    1383 
   Size:        100     434      99     414      54     282

  Fragments arranged by size: 

                 434     414     282     100      99      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     114     146    1104    1136    1383 
   Size:        114      32     958      32     247

  Fragments arranged by size: 

                 958     247     114      32      32
 
HincII GTy'rAC

Cuts at:      0     657    1383 
   Size:        657     726
 
KasI G'GCGC_C

Cuts at:      0      96    1383 
   Size:         96    1287
 
KpnI G_GTAC'C

Cuts at:      0     162    1383 
   Size:        162    1221
 
MspA1I CmG'CkG

Cuts at:      0      42    1194    1383 
   Size:         42    1152     189

  Fragments arranged by size: 

                1152     189      42
 
NaeI GCC'GGC

Cuts at:      0     634    1383 
   Size:        634     749
 
NarI GG'CG_CC

Cuts at:      0      97    1383 
   Size:         97    1286
 
NgoMIV G'CCGG_C

Cuts at:      0     632    1383 
   Size:        632     751
 
NspI r_CATG'y

Cuts at:      0     858     888     898    1383 
   Size:        858      30      10     485

  Fragments arranged by size: 

                 858     485      30      10
 
PasI CC'CwG_GG

Cuts at:      0    1134    1383 
   Size:       1134     249
 
PfoI T'CCnGG_A

Cuts at:      0     164     776    1383 
   Size:        164     612     607

  Fragments arranged by size: 

                 612     607     164
 
PpuMI rG'GwC_Cy

Cuts at:      0     489    1383 
   Size:        489     894
 
PspOMI G'GGCC_C

Cuts at:      0      13    1383 
   Size:         13    1370
 
PstI C_TGCA'G

Cuts at:      0     218    1169    1383 
   Size:        218     951     214

  Fragments arranged by size: 

                 951     218     214
 
SacI G_AGCT'C

Cuts at:      0     308    1383 
   Size:        308    1075
 
SapI GCTCTTCn'nnn_

Cuts at:      0     614    1028    1383 
   Size:        614     414     355

  Fragments arranged by size: 

                 614     414     355
 
SfcI C'TryA_G

Cuts at:      0     214    1165    1383 
   Size:        214     951     218

  Fragments arranged by size: 

                 951     218     214
 
SfoI GGC'GCC

Cuts at:      0      98    1383 
   Size:         98    1285
 
SmaI CCC'GGG

Cuts at:      0     487    1069    1383 
   Size:        487     582     314

  Fragments arranged by size: 

                 582     487     314
 
SmlI C'TyrA_G

Cuts at:      0     307     343     544    1258    1345    1383 
   Size:        307      36     201     714      87      38

  Fragments arranged by size: 

                 714     307     201      87      38      36
 
SphI G_CATG'C

Cuts at:      0     888    1383 
   Size:        888     495
 
StuI AGG'CCT

Cuts at:      0     143    1383 
   Size:        143    1240
 
StyI C'CwwG_G

Cuts at:      0     809    1088    1383 
   Size:        809     279     295

  Fragments arranged by size: 

                 809     295     279
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1283    1383 
   Size:       1283     100
 
TatI w'GTAC_w

Cuts at:      0     146     180     378    1383 
   Size:        146      34     198    1005

  Fragments arranged by size: 

                1005     198     146      34
 
Tth111I GACn'n_nGTC

Cuts at:      0     271    1383 
   Size:        271    1112
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381    1383 
   Size:        381    1002
 
XhoI C'TCGA_G

Cuts at:      0    1345    1383 
   Size:       1345      38
 
XmaI C'CCGG_G

Cuts at:      0     485    1067    1383 
   Size:        485     582     316

  Fragments arranged by size: 

                 582     485     316
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI    AflII     AgeI     AhdI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     ClaI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PasI     PfoI    PpuMI   PspOMI     PstI     SacI     SapI
     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI   AflIII     AleI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BmtI    BsaAI    BsaBI
    BsiEI    BsiWI    BspEI    BspMI    BsrDI   BssHII    BstBI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EagI
    EcoRI     FseI    FspAI  HindIII     HpaI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     TstI     XbaI     XmnI     ZraI
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