Restriction map for pF1KB5845
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cac8I       CelII       CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MboII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI          396  GCGG                 CCGC(-3/-1)
AflIII        109  ACATGT               A^C[GA][CT]GT
AlwI          189  GATCC                GGATC(4/5)
AlwNI         317  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          280  CCTGAGG              CC^T[ACGT]AGG
ApaI          129  GGGCCC               GGGCC^C
AvaII         330  GGACC                G^G[AT]CC
BanII         129  GGGCCC               G[GA]GC[CT]^C
BcnI          219  CCGGG                CC^[GC]GG
BseRI         103  GAGGAG               GAGGAG(10/8)
BspLU11I      109  ACATGT               A^CATGT
BspMI         392  ACCTGC               ACCTGC(4/8)
BsrFI         332  ACCGGC               [GA]^CCGG[CT]
BstYI         188  AGATCC               [GA]^GATC[CT]
Bsu36I        280  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        332  ACCGGC               [GA]^CCGG[CT]
DpnII         189  GATC                 ^GATC
EaeI          372  TGGCCG               [CT]^GGCC[GA]
Eco81I        280  CCTGAGG              CC^T[ACGT]AGG
FauI          396  GCGGG                CCCGC(4/6)
HgaI          147  GACGC                GACGC(5/10)
HinfI          54  GAATC                G^A[ACGT]TC
MaeII         346  ACGT                 A^CGT
MboI          189  GATC                 ^GATC
MflI          188  AGATCC               [GA]^GATC[CT]
MwoI          218  GCCGGGGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          219  CCGGG                CC^[GC]GG
NdeII         189  GATC                 ^GATC
NlaIII        110  CATG                 CATG^
NspI          109  ACATGT               [GA]CATG^[CT]
PflMI           6  CCACTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          184  CTGCAG               CTGCA^G
Sau3AI        189  GATC                 ^GATC
SfaNI         355  GATGC                GCATC(5/9)
SfcI          184  CTGCAG               C^T[GA][CT]AG
TaqI           92  TCGA                 T^CGA
TfiI           54  GAATC                G^A[AT]TC
TthHB8I        92  TCGA                 T^CGA
Van91I          6  CCACTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          192  CCAGTTTCAGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         188  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BmyI          129  GGGCCC               G[AGT]GC[ACT]^C
              243  GGGCAC              
BpmI          210  CTCCAG               CTGGAG(16/14)
              151  CTGGAG              
BsaJI         126  CCAGGG               C^C[ACGT][ACGT]GG
              219  CCGGGG              
Bsp1286I      129  GGGCCC               G[AGT]GC[ACT]^C
              243  GGGCAC              
DraII         128  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              129  GGGCCCC             
EcoO109I      128  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              129  GGGCCCC             
HaeIII        130  GGCC                 GG^CC
              373  GGCC                
HapII         219  CCGG                 C^CGG
              333  CCGG                
HpaII         219  CCGG                 C^CGG
              333  CCGG                
MaeIII        145  GTGAC                ^GT[ACGT]AC
              343  GTGAC               
MspA1I        181  CAGCTG               C[AC]G^C[GT]G
              317  CAGCTG              
MspI          219  CCGG                 C^CGG
              333  CCGG                
NlaIV         129  GGGCCC               GG[ACGT]^[ACGT]CC
              130  GGCCCC              
PvuII         181  CAGCTG               CAG^CTG
              317  CAGCTG              
Tsp45I        145  GTGAC                ^GT[GC]AC
              343  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HphI        
ItaI        MnlI        MvaI        Sau96I      ScrFI       
TseI        TspRI       
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