Restriction map for pF1KB5839
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BpmI        
BsaAI       BsaBI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Cac8I       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FseI        HapII       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcyI          460  GACGCC               G[GA]^CG[CT]C
AfaI          405  GTAC                 GT^AC
AocI          334  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       371  AGCGCT               AGC^GCT
AvaII         360  GGACC                G^G[AT]CC
AviII         281  TGCGCA               TGC^GCA
AvrII         219  CCTAGG               C^CTAGG
BclI          497  TGATCA               T^GATCA
BlnI          219  CCTAGG               C^CTAGG
BlpI          468  GCTGAGC              GC^T[ACGT]AGC
BmyI          167  GTGCCC               G[AGT]GC[ACT]^C
Bpu1102I      468  GCTGAGC              GC^T[ACGT]AGC
BsaHI         460  GACGCC               G[GA]^CG[CT]C
BsaI          206  GAGACC               GGTCTC(1/5)
BsgI          489  CTGCAC               GTGCAG(16/14)
BsmFI         593  GGGAC                GGGAC(10/14)
Bsp1286I      167  GTGCCC               G[AGT]GC[ACT]^C
BsrDI         260  GCAATG               GCAATG(2/0)
BstBI         317  TTCGAA               TT^CGAA
Bsu36I        334  CCTAAGG              CC^T[ACGT]AGG
CelII         468  GCTGAGC              GC^T[ACGT]AGC
DraII         237  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          179  CCATGG               C^C[GA][CT]GG
EaeI          533  TGGCCG               [CT]^GGCC[GA]
Eco47III      371  AGCGCT               AGC^GCT
Eco81I        334  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      237  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FbaI          497  TGATCA               T^GATCA
FspI          281  TGCGCA               TGC^GCA
HgaI          460  GACGC                GACGC(5/10)
Hin1I         460  GACGCC               G[GA]^CG[CT]C
MaeIII          7  GTGAC                ^GT[ACGT]AC
MslI          291  CACCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          169  GCCCTGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          179  CCATGG               C^CATGG
NspV          317  TTCGAA               TT^CGAA
PstI           55  CTGCAG               CTGCA^G
RsaI          405  GTAC                 GT^AC
ScaI          404  AGTACT               AGT^ACT
SfaNI         377  GCATC                GCATC(5/9)
SfuI          317  TTCGAA               TT^CGAA
TaqI          318  TCGA                 T^CGA
TfiI          315  GATTC                G^A[AT]TC
Tsp45I          7  GTGAC                ^GT[GC]AC
TthHB8I       318  TCGA                 T^CGA
XbaI          432  TCTAGA               T^CTAGA
XcmI          579  CCACAGCAACGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BfaI          220  CTAG                 C^TAG
              433  CTAG                
BsmAI         206  GAGAC                GTCTC(1/5)
              352  GTCTC               
BsrI          186  CCAGT                ACTGG(1/-1)
              306  CCAGT               
BssKI         171  CCTGG                ^CC[ACGT]GG
              234  CCTGG               
BstF5I        339  GGATG                GGATG(2/0)
              456  GGATG               
BstNI         171  CCTGG                CC^[AT]GG
              234  CCTGG               
DpnII         430  GATC                 ^GATC
              498  GATC                
EcoRII        171  CCTGG                ^CC[AT]GG
              234  CCTGG               
EcoT14I       179  CCATGG               C^C[AT][AT]GG
              219  CCTAGG              
FokI          339  GGATG                GGATG(9/13)
              456  GGATG               
HaeII         213  AGCGCC               [GA]GCGC^[CT]
              371  AGCGCT              
HphI            6  GGTGA                GGTGA(8/7)
              567  GGTGA               
MaeI          220  CTAG                 C^TAG
              433  CTAG                
MboI          430  GATC                 ^GATC
              498  GATC                
MvaI          171  CCTGG                CC^[AT]GG
              234  CCTGG               
NdeII         430  GATC                 ^GATC
              498  GATC                
NlaIII        180  CATG                 CATG^
              279  CATG                
PleI           24  GAGTC                GAGTC(4/5)
              538  GAGTC               
Sau3AI        430  GATC                 ^GATC
              498  GATC                
ScrFI         171  CCTGG                CC^[ACGT]GG
              234  CCTGG               
StyI          179  CCATGG               C^C[AT][AT]GG
              219  CCTAGG              
TspRI         187  CAGTG                CA[GC]TG[ACGT][ACGT]^
              307  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        BbsI        BbvI        BpuAI       
BsaJI       CfoI        Cfr13I      DdeI        Fnu4HI      
HaeIII      HhaI        HinP1I      HinfI       ItaI        
MboII       MnlI        NlaIV       Sau96I      SfcI        
TseI        
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