Restriction map for pF1KB5819
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbsI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MslI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SbfI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          782  GTCGAC               GT^[AC][GT]AC
Aor51HI       911  AGCGCT               AGC^GCT
ApaI          470  GGGCCC               GGGCC^C
Asp700I      1143  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1101  TGGCCA               TGG^CCA
BbrPI         406  CACGTG               CAC^GTG
BbvCI         652  GCTGAGG              CCTCAGC(-5/-2)
BcgI          960  GCAGCGGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI           73  GCCCGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         406  CACGTG               [CT]AC^GT[GA]
BsgI         1231  CTGCAC               GTGCAG(16/14)
BsmBI         190  GAGACG               CGTCTC(1/5)
BspLU11I      449  ACATGT               A^CATGT
BsrBI         874  GAGCGG               CCGCTC(-3/-3)
BssSI         307  CACGAG               CACGAG(-5/-1)
BstAPI        918  GCAGCGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1029  CATCC                GGATG(2/0)
DsaI         1118  CCATGG               C^C[GA][CT]GG
Eco47III      911  AGCGCT               AGC^GCT
FokI         1029  CATCC                GGATG(9/13)
HincII        782  GTCGAC               GT[CT]^[GA]AC
HindII        782  GTCGAC               GT[CT]^[GA]AC
MluNI        1101  TGGCCA               TGG^CCA
MscI         1101  TGGCCA               TGG^CCA
NcoI         1118  CCATGG               C^CATGG
PflMI        1185  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         406  CACGTG               CAC^GTG
PmlI          406  CACGTG               CAC^GTG
SacI         1091  GAGCTC               GAGCT^C
SalI          782  GTCGAC               G^TCGAC
ScaI          600  AGTACT               AGT^ACT
SmaI          483  CCCGGG               CCC^GGG
SmlI         1054  CTCAAG               C^T[CT][GA]AG
SphI          923  GCATGC               GCATG^C
TaqI          783  TCGA                 T^CGA
TfiI          613  GAATC                G^A[AT]TC
TthHB8I       783  TCGA                 T^CGA
Van91I       1185  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         483  CCCGGG               C^CCGGG
XmaI          483  CCCGGG               C^CCGGG
XmnI         1143  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        407  ACGTGT               A^C[GA][CT]GT
              449  ACATGT              
AlwI           42  GGATC                GGATC(4/5)
              966  GGATC               
AlwNI         814  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1186  CAGCTTCTG           
AspHI         304  GAGCAC               G[AT]GC[AT]^C
             1091  GAGCTC              
AvaI          483  CCCGGG               C^[CT]CG[GA]G
              868  CCCGAG              
BfaI           61  CTAG                 C^TAG
              373  CTAG                
BsaI          107  GAGACC               GGTCTC(1/5)
              862  GAGACC              
BsiHKAI       304  GAGCAC               G[AT]GC[AT]^C
             1091  GAGCTC              
BsoBI         483  CCCGGG               C^[CT]CG[GA]G
              868  CCCGAG              
BsrFI         848  GCCGGC               [GA]^CCGG[CT]
              953  GCCGGC              
Cfr10I        848  GCCGGC               [GA]^CCGG[CT]
              953  GCCGGC              
EcoRI         327  GAATTC               G^AATTC
              698  GAATTC              
EcoT14I       111  CCTTGG               C^C[AT][AT]GG
             1118  CCATGG              
HaeII         436  AGCGCC               [GA]GCGC^[CT]
              911  AGCGCT              
MaeI           61  CTAG                 C^TAG
              373  CTAG                
MaeII         407  ACGT                 A^CGT
              780  ACGT                
MaeIII        167  GTGAC                ^GT[ACGT]AC
              313  GTCAC               
MseI          508  TTAA                 T^TAA
              578  TTAA                
NaeI          848  GCCGGC               GCC^GGC
              953  GCCGGC              
NgoMIV        848  GCCGGC               G^CCGGC
              953  GCCGGC              
NspI          449  ACATGT               [GA]CATG^[CT]
              923  GCATGC              
PleI          154  GACTC                GAGTC(4/5)
              753  GACTC               
PpuMI         639  GGGTCCC              [GA]G^G[AT]CC[CT]
             1106  AGGACCC             
PvuII        1133  CAGCTG               CAG^CTG
             1153  CAGCTG              
StyI          111  CCTTGG               C^C[AT][AT]GG
             1118  CCATGG              
Tru9I         508  TTAA                 T^TAA
              578  TTAA                
Tsp45I        167  GTGAC                ^GT[GC]AC
              313  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        Tsp509I     TspRI       
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