Restriction map for pF1KB5819
 (Linear) (Six-base) MAPSORT of: KIBB5819.seq  Check: 5652  from: 1  to: 1236



KIBB5819.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:      0     783    1236 
   Size:        783     453
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    1006    1236 
   Size:        617     389     230

  Fragments arranged by size: 

                 617     389     230
 
AfeI AGC'GCT

Cuts at:      0     913    1236 
   Size:        913     323
 
AflIII A'CryG_T

Cuts at:      0     407     449    1236 
   Size:        407      42     787

  Fragments arranged by size: 

                 787     407      42
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     416    1236 
   Size:        416     820
 
AlwNI CAG_nnn'CTG

Cuts at:      0     819    1191    1236 
   Size:        819     372      45

  Fragments arranged by size: 

                 819     372      45
 
ApaI G_GGCC'C

Cuts at:      0     474    1236 
   Size:        474     762
 
ApoI r'AATT_y

Cuts at:      0     321     327     698    1236 
   Size:        321       6     371     538

  Fragments arranged by size: 

                 538     371     321       6
 
AvaI C'yCGr_G

Cuts at:      0     483     868    1236 
   Size:        483     385     368

  Fragments arranged by size: 

                 483     385     368
 
BanII G_rGCy'C

Cuts at:      0     474     555     777    1095    1236 
   Size:        474      81     222     318     141

  Fragments arranged by size: 

                 474     318     222     141      81
 
BbvCI CC'TCA_GC

Cuts at:      0     653    1236 
   Size:        653     583
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     949     983    1236 
   Size:        949      34     253

  Fragments arranged by size: 

                 949     253      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79    1236 
   Size:         79    1157
 
Bme1580I G_kGCm'C

Cuts at:      0     474     951    1236 
   Size:        474     477     285

  Fragments arranged by size: 

                 477     474     285
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48     752    1236 
   Size:         48     704     484

  Fragments arranged by size: 

                 704     484      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     660    1236 
   Size:        660     576
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     141     630     665    1087    1236 
   Size:        125      16     489      35     422     149

  Fragments arranged by size: 

                 489     422     149     125      35      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     475     653    1128    1236 
   Size:        475     178     475     108

  Fragments arranged by size: 

                 475     475     178     108
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1236 
   Size:       1039     197
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     101     856    1236 
   Size:        101     755     380

  Fragments arranged by size: 

                 755     380     101
 
BsaAI yAC'GTr

Cuts at:      0     408    1236 
   Size:        408     828
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     227     244     257     274    1236 
   Size:        227      17      13      17     962

  Fragments arranged by size: 

                 962     227      17      17      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     886    1074    1236 
   Size:        202     684     188     162

  Fragments arranged by size: 

                 684     202     188     162
 
BseYI C'CCAG_C

Cuts at:      0      55    1236 
   Size:         55    1181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1216    1236 
   Size:       1216      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     308    1095    1236 
   Size:        308     787     141

  Fragments arranged by size: 

                 787     308     141
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     184    1236 
   Size:        184    1052
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     474     555     777     951    1095    1236 
   Size:        308     166      81     222     174     144     141

  Fragments arranged by size: 

                 308     222     174     166     144     141      81
 
BsrBI CCG'CTC

Cuts at:      0     876    1236 
   Size:        876     360
 
BsrFI r'CCGG_y

Cuts at:      0     848     953    1236 
   Size:        848     105     283

  Fragments arranged by size: 

                 848     283     105
 
BssSI C'ACGA_G

Cuts at:      0     307    1236 
   Size:        307     929
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     924    1236 
   Size:        924     312
 
BtgI C'CryG_G

Cuts at:      0    1118    1236 
   Size:       1118     118
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      97    1236 
   Size:         97    1139
 
BtsI GCAGTG_nn'

Cuts at:      0     171     419     434    1236 
   Size:        171     248      15     802

  Fragments arranged by size: 

                 802     248     171      15
 
EaeI y'GGCC_r

Cuts at:      0     465     836    1101    1236 
   Size:        465     371     265     135

  Fragments arranged by size: 

                 465     371     265     135
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     179     216     838    1205    1236 
   Size:         59     120      37     622     367      31

  Fragments arranged by size: 

                 622     367     120      59      37      31
 
EcoICRI GAG'CTC

Cuts at:      0    1093    1236 
   Size:       1093     143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     141     617     630     665    1006    1087    1236 
   Size:        125      16     476      13      35     341      81     149

  Fragments arranged by size: 

                 476     341     149     125      81      35      16      13
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     471     640    1107    1236 
   Size:        471     169     467     129

  Fragments arranged by size: 

                 471     467     169     129
 
EcoRI G'AATT_C

Cuts at:      0     327     698    1236 
   Size:        327     371     538

  Fragments arranged by size: 

                 538     371     327
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1221    1236 
   Size:       1221      15
 
HaeII r_GCGC'y

Cuts at:      0     440     915    1236 
   Size:        440     475     321

  Fragments arranged by size: 

                 475     440     321
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     139     148     171     193     225     227     259 
   Size:        116      23       9      23      22      32       2      32

Cuts at:    259     514     546    1236 
   Size:        255      32     690

  Fragments arranged by size: 

                 690     255     116      32      32      32      23      23
                  22       9       2
 
HincII GTy'rAC

Cuts at:      0     784    1236 
   Size:        784     452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     159    1157    1236 
   Size:        159     998      79

  Fragments arranged by size: 

                 998     159      79
 
MscI TGG'CCA

Cuts at:      0    1103    1236 
   Size:       1103     133
 
MspA1I CmG'CkG

Cuts at:      0     816     945     963    1135    1155    1236 
   Size:        816     129      18     172      20      81

  Fragments arranged by size: 

                 816     172     129      81      20      18
 
NaeI GCC'GGC

Cuts at:      0     850     955    1236 
   Size:        850     105     281

  Fragments arranged by size: 

                 850     281     105
 
NcoI C'CATG_G

Cuts at:      0    1118    1236 
   Size:       1118     118
 
NgoMIV G'CCGG_C

Cuts at:      0     848     953    1236 
   Size:        848     105     283

  Fragments arranged by size: 

                 848     283     105
 
NspI r_CATG'y

Cuts at:      0     453     927    1236 
   Size:        453     474     309

  Fragments arranged by size: 

                 474     453     309
 
PciI A'CATG_T

Cuts at:      0     449    1236 
   Size:        449     787
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1191    1236 
   Size:       1191      45
 
PfoI T'CCnGG_A

Cuts at:      0     102    1236 
   Size:        102    1134
 
PmlI CAC'GTG

Cuts at:      0     408    1236 
   Size:        408     828
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1210    1236 
   Size:       1210      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     640    1107    1236 
   Size:        640     467     129

  Fragments arranged by size: 

                 640     467     129
 
PspOMI G'GGCC_C

Cuts at:      0     470    1236 
   Size:        470     766
 
PstI C_TGCA'G

Cuts at:      0     426     815     962    1236 
   Size:        426     389     147     274

  Fragments arranged by size: 

                 426     389     274     147
 
PvuII CAG'CTG

Cuts at:      0    1135    1155    1236 
   Size:       1135      20      81

  Fragments arranged by size: 

                1135      81      20
 
SacI G_AGCT'C

Cuts at:      0    1095    1236 
   Size:       1095     141
 
SalI G'TCGA_C

Cuts at:      0     782    1236 
   Size:        782     454
 
SanDI GG'GwC_CC

Cuts at:      0     640    1236 
   Size:        640     596
 
SapI GCTCTTCn'nnn_

Cuts at:      0      59     838    1205    1236 
   Size:         59     779     367      31

  Fragments arranged by size: 

                 779     367      59      31
 
ScaI AGT'ACT

Cuts at:      0     602    1236 
   Size:        602     634
 
SfcI C'TryA_G

Cuts at:      0     422     811     958    1236 
   Size:        422     389     147     278

  Fragments arranged by size: 

                 422     389     278     147
 
SmaI CCC'GGG

Cuts at:      0     485    1236 
   Size:        485     751
 
SmlI C'TyrA_G

Cuts at:      0    1054    1236 
   Size:       1054     182
 
SphI G_CATG'C

Cuts at:      0     927    1236 
   Size:        927     309
 
StyI C'CwwG_G

Cuts at:      0     111    1118    1236 
   Size:        111    1007     118

  Fragments arranged by size: 

                1007     118     111
 
TatI w'GTAC_w

Cuts at:      0     600    1236 
   Size:        600     636
 
XmaI C'CCGG_G

Cuts at:      0     483    1236 
   Size:        483     753
 
XmnI GAAnn'nnTTC

Cuts at:      0    1147    1236 
   Size:       1147      89
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApaI     ApoI
     AvaI    BanII    BbvCI     BcgI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrFI    BssSI   BstAPI
     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     MmeI     MscI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     SalI    SanDI
     SapI     ScaI     SfcI     SmaI     SmlI     SphI     StyI     TatI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     ZraI
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