Restriction map for pF1KB7987
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AviII       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        MaeI        MfeI        MluI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       1142  GGTACC               G^GTACC
AccIII        287  TCCGGA               T^CCGGA
Asp718I      1142  GGTACC               G^GTACC
AspI          542  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1142  GGTACC               G^G[CT][GA]CC
BglI          145  GCCACTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          256  CTGGAG               CTGGAG(16/14)
BsaBI         123  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         287  TCCGGA               [AT]^CCGG[AT]
BseAI         287  TCCGGA               T^CCGGA
BsmBI         379  GAGACG               CGTCTC(1/5)
BspEI         287  TCCGGA               T^CCGGA
BspHI          56  TCATGA               T^CATGA
BsrBI         634  GAGCGG               CCGCTC(-3/-3)
BsrFI         419  ACCGGC               [GA]^CCGG[CT]
BstAPI        669  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        419  ACCGGC               [GA]^CCGG[CT]
DdeI         1115  CTGAG                C^T[ACGT]AG
HincII       1051  GTCAAC               GT[CT]^[GA]AC
HindII       1051  GTCAAC               GT[CT]^[GA]AC
KpnI         1142  GGTACC               GGTAC^C
MamI          123  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          287  TCCGGA               T^CCGGA
PflFI         542  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1076  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1184  CAGCTG               CAG^CTG
RcaI           56  TCATGA               T^CATGA
SacI         1117  GAGCTC               GAGCT^C
SbfI          666  CCTGCAGG             CCTGCA^GG
SfiI          144  GGCCACTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          794  CCCGGG               CCC^GGG
Sse8387I      666  CCTGCAGG             CCTGCA^GG
StuI          581  AGGCCT               AGG^CCT
Tth111I       542  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1076  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          245  CCAGGGAGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         794  CCCGGG               C^CCGGG
XmaI          794  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AhdI          163  GACGCGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              316  GACATCAAGTC         
Alw44I        871  GTGCAC               G^TGCAC
             1108  GTGCAC              
ApaLI         871  GTGCAC               G^TGCAC
             1108  GTGCAC              
AspEI         163  GACGCGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              316  GACATCAAGTC         
AvaI          794  CCCGGG               C^[CT]CG[GA]G
             1288  CCCGAG              
BalI          143  TGGCCA               TGG^CCA
             1272  TGGCCA              
BsaI          523  GAGACC               GGTCTC(1/5)
             1301  GAGACC              
BseRI         376  GAGGAG               GAGGAG(10/8)
              985  GAGGAG              
BsiEI         326  CGGCCG               CG[GA][CT]^CG
              383  CGGCCG              
BsoBI         794  CCCGGG               C^[CT]CG[GA]G
             1288  CCCGAG              
BspMI         674  ACCTGC               ACCTGC(4/8)
             1139  GCAGGT              
BsrDI          92  CATTGC               GCAATG(2/0)
              342  CATTGC              
BssSI         461  CACGAG               CACGAG(-5/-1)
              874  CACGAG              
DrdI          745  GACTTCACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1096  GACCCCGCCGTC        
EagI          326  CGGCCG               C^GGCCG
              383  CGGCCG              
Eam1105I      163  GACGCGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              316  GACATCAAGTC         
EarI          601  CTCTTC               CTCTTC(1/4)
              679  CTCTTC              
EclXI         326  CGGCCG               C^GGCCG
              383  CGGCCG              
Eco52I        326  CGGCCG               C^GGCCG
              383  CGGCCG              
EcoT14I       527  CCTTGG               C^C[AT][AT]GG
              578  CCAAGG              
Ksp632I       601  CTCTTC               CTCTTC(1/4)
              679  CTCTTC              
MaeIII        697  GTGAC                ^GT[ACGT]AC
             1313  GTGAC               
MluNI         143  TGGCCA               TGG^CCA
             1272  TGGCCA              
MscI          143  TGGCCA               TGG^CCA
             1272  TGGCCA              
MslI          972  CATCGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1306  CACGCCAGTG          
PstI          649  CTGCAG               CTGCA^G
              667  CTGCAG              
SapI          600  GCTCTTC              GCTCTTC(1/4)
              678  GCTCTTC             
StyI          527  CCTTGG               C^C[AT][AT]GG
              578  CCAAGG              
Tsp45I        697  GTGAC                ^GT[GC]AC
             1313  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaII       
BamHI       BanII       BbvI        BcnI        BmyI        
BsaHI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KspI        
MaeII       MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
TspRI       TthHB8I     XhoII       
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