Restriction map for pF1KB7958
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BcnI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflII         988  CTTAAG               C^TTAAG
AhdI          514  GACTTGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1099  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         514  GACTTGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         526  GGATCC               G^GATCC
BclI          678  TGATCA               T^GATCA
BfaI          488  CTAG                 C^TAG
BfrI          988  CTTAAG               C^TTAAG
BsaI          581  GGTCTC               GGTCTC(1/5)
BsaJI         123  CCACGG               C^C[ACGT][ACGT]GG
BseRI         778  CTCCTC               GAGGAG(10/8)
BsiYI         157  CCTGTTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          157  CCTGTTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I      312  TGTACA               T^GTACA
BspHI         327  TCATGA               T^CATGA
BsrDI         945  CATTGC               GCAATG(2/0)
BsrGI         312  TGTACA               T^GTACA
BstXI         123  CCACGGCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         526  GGATCC               [GA]^GATC[CT]
Cac8I          19  GCACGC               GC[ACGT]^[ACGT]GC
DdeI          966  CTCAG                C^T[ACGT]AG
DsaI          123  CCACGG               C^C[GA][CT]GG
Eam1105I      514  GACTTGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         157  CCTGTTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          678  TGATCA               T^GATCA
HaeIII       1149  GGCC                 GG^CC
HapII        1147  CCGG                 C^CGG
HpaII        1147  CCGG                 C^CGG
HphI          774  TCACC                GGTGA(8/7)
MaeI          488  CTAG                 C^TAG
MaeII         588  ACGT                 A^CGT
MflI          526  GGATCC               [GA]^GATC[CT]
MspI         1147  CCGG                 C^CGG
PleI           64  GAGTC                GAGTC(4/5)
RcaI          327  TCATGA               T^CATGA
SapI          650  GAAGAGC              GCTCTTC(1/4)
SspBI         312  TGTACA               T^GTACA
SspI          564  AATATT               AAT^ATT
TaqI          801  TCGA                 T^CGA
TthHB8I       801  TCGA                 T^CGA
XcmI          461  CCAAGCACTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         526  GGATCC               [GA]^GATC[CT]
XmnI         1099  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI           17  CCGC                 CCGC(-3/-1)
              128  GCGG                
AflIII        173  ACATGT               A^C[GA][CT]GT
              247  ACATGT              
AluI          352  AGCT                 AG^CT
              705  AGCT                
AlwI          527  GATCC                GGATC(4/5)
              526  GGATC               
AlwNI        1059  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1103  CAGTTCCTG           
AvaII          39  GGACC                G^G[AT]CC
              493  GGACC               
BbvI         1169  GCAGC                GCAGC(8/12)
              905  GCTGC               
BciVI         381  GGATAC               GTATCC(6/5)
              321  GTATCC              
BsmFI          84  GGGAC                GGGAC(10/14)
              993  GGGAC               
BspLU11I      173  ACATGT               A^CATGT
              247  ACATGT              
BstAPI        718  GCAAACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1058  GCAGAGACTGC         
Cfr13I         39  GGACC                G^G[ACGT]CC
              493  GGACC               
DraII          38  AGGACCC              [GA]G^G[ACGT]CC[CT]
              492  AGGACCC             
EarI           44  CTCTTC               CTCTTC(1/4)
              650  GAAGAG              
EcoO109I       38  AGGACCC              [GA]G^G[ACGT]CC[CT]
              492  AGGACCC             
Fnu4HI        905  GCTGC                GC^[ACGT]GC
             1169  GCAGC               
HincII         66  GTCAAC               GT[CT]^[GA]AC
             1009  GTTGAC              
HindII         66  GTCAAC               GT[CT]^[GA]AC
             1009  GTTGAC              
ItaI          905  GCTGC                GC^[ACGT]GC
             1169  GCAGC               
Ksp632I        44  CTCTTC               CTCTTC(1/4)
              650  GAAGAG              
MwoI          718  GCAAACACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1058  GCAGAGACTGC         
NdeI          442  CATATG               CA^TATG
              932  CATATG              
PpuMI          38  AGGACCC              [GA]G^G[AT]CC[CT]
              492  AGGACCC             
PstI          401  CTGCAG               CTGCA^G
              906  CTGCAG              
Sau96I         39  GGACC                G^G[ACGT]CC
              493  GGACC               
SfaNI        1159  GATGC                GCATC(5/9)
              655  GCATC               
SmlI          217  CTTGAG               C^T[CT][GA]AG
              988  CTTAAG              
TfiI          119  GAATC                G^A[AT]TC
              712  GAATC               
TseI          905  GCTGC                G^C[AT]GC
             1169  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AfaI        ApoI        BpmI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       DpnII       
EcoRII      FokI        HinfI       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
NdeII       NlaIII      NlaIV       NspI        RsaI        
Sau3AI      ScrFI       SfcI        Tru9I       Tsp45I      
Tsp509I     TspRI       
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