Restriction map for pF1KB7940
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaHI       BseRI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MluI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        367  TCCGGA               T^CCGGA
AciI            8  CCGC                 CCGC(-3/-1)
Alw44I        511  GTGCAC               G^TGCAC
AlwI          201  GATCC                GGATC(4/5)
ApaLI         511  GTGCAC               G^TGCAC
AspI          162  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          419  CCCGGG               C^[CT]CG[GA]G
BalI          254  TGGCCA               TGG^CCA
BglII         327  AGATCT               A^GATCT
BpmI          170  CTCCAG               CTGGAG(16/14)
BsaAI         558  CACGTA               [CT]AC^GT[GA]
BsaBI         123  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          579  GGTCTC               GGTCTC(1/5)
BsaWI         367  TCCGGA               [AT]^CCGG[AT]
BseAI         367  TCCGGA               T^CCGGA
BsgI          247  GTGCAG               GTGCAG(16/14)
BsiEI           5  CGACCG               CG[GA][CT]^CG
BsmFI         417  GTCCC                GGGAC(10/14)
BsoBI         419  CCCGGG               C^[CT]CG[GA]G
BspEI         367  TCCGGA               T^CCGGA
BssHII        313  GCGCGC               G^CGCGC
BstBI         290  TTCGAA               TT^CGAA
DdeI          485  CTGAG                C^T[ACGT]AG
DraII         415  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        689  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          254  TGGCCA               [CT]^GGCC[GA]
EcoO109I      415  GGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeII         188  AGCGCC               [GA]GCGC^[CT]
HgaI          162  GACGC                GACGC(5/10)
HphI          156  GGTGA                GGTGA(8/7)
MaeII         559  ACGT                 A^CGT
MamI          123  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          623  CAATTG               C^AATTG
MluNI         254  TGGCCA               TGG^CCA
MroI          367  TCCGGA               T^CCGGA
MscI          254  TGGCCA               TGG^CCA
MseI          382  TTAA                 T^TAA
MslI          534  CATGCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          623  CAATTG               C^AATTG
NspI          533  ACATGC               [GA]CATG^[CT]
NspV          290  TTCGAA               TT^CGAA
PflFI         162  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         177  CCAGCGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         415  GGGTCCC              [GA]G^G[AT]CC[CT]
SexAI         575  ACCAGGT              A^CC[AT]GGT
SfuI          290  TTCGAA               TT^CGAA
SmaI          419  CCCGGG               CCC^GGG
Tru9I         382  TTAA                 T^TAA
Tsp509I       624  AATT                 ^AATT
Tth111I       162  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        177  CCAGCGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          576  CCAGGTCTCCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         419  CCCGGG               C^CCGGG
XmaI          419  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII         181  CGCG                 CG^CG
              314  CGCG                
AspHI          22  GAGCAC               G[AT]GC[AT]^C
              511  GTGCAC              
BbsI          159  GAAGAC               GAAGAC(2/6)
              448  GAAGAC              
BbvI          249  GCAGC                GCAGC(8/12)
              136  GCTGC               
BmyI           22  GAGCAC               G[AGT]GC[ACT]^C
              511  GTGCAC              
BpuAI         159  GAAGAC               GAAGAC(2/6)
              448  GAAGAC              
BsiHKAI        22  GAGCAC               G[AT]GC[AT]^C
              511  GTGCAC              
BsiYI          96  CCAGAGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              177  CCAGCGCGTGG         
BslI           96  CCAGAGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              177  CCAGCGCGTGG         
BsmAI         168  GTCTC                GTCTC(1/5)
              580  GTCTC               
Bsp1286I       22  GAGCAC               G[AGT]GC[ACT]^C
              511  GTGCAC              
BstF5I        129  CATCC                GGATG(2/0)
              501  CATCC               
BstUI         181  CGCG                 CG^CG
              314  CGCG                
BstXI          11  CCATGTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              622  CCAATTGAGTGG        
BstYI         200  AGATCC               [GA]^GATC[CT]
              327  AGATCT              
DpnII         201  GATC                 ^GATC
              328  GATC                
Fnu4HI        136  GCTGC                GC^[ACGT]GC
              249  GCAGC               
FokI          129  CATCC                GGATG(9/13)
              501  CATCC               
ItaI          136  GCTGC                GC^[ACGT]GC
              249  GCAGC               
MaeIII        236  GTGAC                ^GT[ACGT]AC
              605  GTGAC               
MboI          201  GATC                 ^GATC
              328  GATC                
MflI          200  AGATCC               [GA]^GATC[CT]
              327  AGATCT              
MspA1I         76  CAGCTG               C[AC]G^C[GT]G
              250  CAGCTG              
MvnI          181  CGCG                 CG^CG
              314  CGCG                
NdeII         201  GATC                 ^GATC
              328  GATC                
PstI          137  CTGCAG               CTGCA^G
              193  CTGCAG              
PvuII          76  CAGCTG               CAG^CTG
              250  CAGCTG              
Sau3AI        201  GATC                 ^GATC
              328  GATC                
SfaNI          81  GATGC                GCATC(5/9)
              301  GATGC               
TaqI           41  TCGA                 T^CGA
              291  TCGA                
TseI          136  GCTGC                G^C[AT]GC
              249  GCAGC               
Tsp45I        236  GTGAC                ^GT[GC]AC
              605  GTGAC               
TthHB8I        41  TCGA                 T^CGA
              291  TCGA                
XhoII         200  AGATCC               [GA]^GATC[CT]
              327  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AfaI        AluI        AvaII       BcnI        BsaJI       
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DsaI        EcoRII      HaeIII      HapII       HhaI        
HinP1I      HpaII       MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       SfcI        TspRI       
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